Phytochemicals, Antioxidant and Antifungal Properties of Acorus calamus, Curcuma mangga, and Allium sativum

Abstract

The purpose of this study to determine the content of phytochemicals, antioxidant and antifungal properties of the combination of Acorus calamus, Curcuma mangga, and Allium sativum. This research was descriptive qualitative, extractions were done by maceration method with ethanol with 3 different combinations (C1, C2 and C3). Phytochemical test reagent included 4 kinds of test, namely: alkaloids, flavonoids, triterpenoids, saponins and tannins. As for the antioxidant test, the method used was DPPH. The concentration used at 25, 50, 100, 200, and 400 ppm. As for the antifungal test conducted on Candida albicans with Kirby-Bauer disc methods with a concentration of 100%, followed by the MIC and MBC test with a concentration of 50%, 25%, 12.5%, 6.25%, 3.13%, 1.56%, 0.78% and 0.39%. Phytochemical test results indicated the presence of the alkaloids, flavonoids and triterpenoids compounds in 3 different combinations (C1, C2 and C3). The highest antioxidant levels founded in C1 (61.75) followed by C3 (47.94) and the lowest levels founded in C2 (42.76). The antifungal test showed the inhibitory zone against C. albicans. The highest inhibitory zone was found in C1 at 5.44 ± 1.78 mm (medium category), followed by C2 at 4.08 ± 0.86 mm (medium category), and C3 at 3.05 ± 0.23 mm (medium category). As for the minimum inhibitory concentration (MIC) value got on the concentration of 0:39% and minimum fungisidal concentration (MFC) values were at a concentration of 0.78%. Key word: Acorus calamu, Allium sativum, Curcuma manga, Antioxidant, antifungal, Candida albicans

Phytochemical Screening and Antibacterial Activity of Acorus calamus L. Extracts

“…Unlike the previous studies, the inhibition zone test for Candida albicans produced the highest value on ethanol extract (3.72 mm/moderate) followed by n-hexane (3.32 mm/medium) and chloroform (2.22 mm/weak) (Muchtaromah et al, 2017). According to Chandra & Prasad (2017), differences in solvents could affect the results of phytochemical compounds drawn during extraction while the number and type of active compounds influenced the biological activity.…”

“…The level of 0.78% was the MBC value of all types of extract for both test bacteria, characterized by the absence of bacterial growth at all (colonies <0.1% of the original inoculum). Previous studies showed that concentrations of 0.39% and 0.78% were also the MIC and MBC values of calamus rhizoma extract against Candida albicans (Muchtaromah, 2017). Moreover it was known that the calamus rhizome extract effectively inhibited both gram positive and negative bacteria (broad spectrum) and fungi.…”

Antioxidant and Antifungal Activity of Jeringau (Acorus calamus L.) Extract In Some Organic Solvents In Vitro

“…The ethanol extract has the highest antioxidant activity may caused by the polar active substance of several antioxidant compounds contained within. The previous research proved that rhizome of jeringau extracted with ethanol has a group of antioxidant compounds such as alkaloids and triterpenoids (Muchtaromah, et al, 2017) where it can function as an antioxidant by donating hydrogen atoms in DPPH.…”

“…Pathogenic microbes, free radicals can also cause infertility, thus antioxidant compounds are needed in neutralizing these free radicals. In previous study, active compounds that can be used as antioxidants and also contained in ethanol extract of jeringau are alkaloids and triterpenoids (Muchtaromah, et al, 2017). Hence this research aims to investigate the best solvent of Jeringau extract that produced the highest antioxidant and antifungi activities based on the polarity.…”

Nanoparticle Characterization of Allium sativum, Curcuma mangga and Acorus calamus as a Basic of Nanotechnology on Jamu Subur Kandungan Madura

“…"Jamu Subur Kandungan" contains 15% garlic (Allium sativum), 15% rhizome of temu mangga (Curcuma mangga), 12% rhizome of jeringau (Acorus calamus), and other materials until 100%, which is well known as fertility enhancer. 7 Our studies have shown that spice blend of garlic (Allium sativum), temu mangga (Curcuma mangga) and jeringau (Acorus calamus) has high antioxidant activity and can be used as antifungal and antibacterial. 7,8 The containing spices in "Jamu Subur Kandungan" are individually considered as remedies for many diseases.…”