Phytochemical study of Salvia grandiflora and Salvia officinalis leaves for establishing prospects for use in medical and pharmaceutical practice

Myha, M. M.; Коshovyi, Oleh; Gamulya, O. V.; Ilina, Тetiana; Borodina, Natalia; Vlasova, Inna

doi:10.15587/2519-4852.2020.197299

Cited by 5 publications

(5 citation statements)

References 14 publications

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“…The content of related impurities (2.8.2), the loss on drying (2.2.32), the total ash content (2.4.16) were determined according to the requirements of the EuPh [18, 23,24].…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

Standardization Parameters of Alfredia nivea KAR.&KIR Herb

Rustemkulov¹,

Махатова²,

Rustemkulova³

et al. 2023

J. Org. Pharm. Chem.

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The Kazakhstan flora is rich in promising poorly-studied plants, which are traditionally used in folk medicine, but their introduction into medical practice requires additional in-depth research using modern scientific methods. Alfredia nivea KAR&KIR of the Asteraceae family, which is used in folk medicine as a neurotropic agent, is an interesting object for introduction into official medical and pharmaceutical practice.Aim. To create new medicines based on Alfredia nivea herb, it is necessary to develop methods for quality control of this raw material, therefore, the aim of the research was to determine the parameters for standardization of the Alfredia nivea KAR & KIR. herb.Materials and methods. The study objects were samples of the A. nivea herb collected in Kungei Alatau, 4.3 km southeast of the Karabulak village, Eastern Karabulak canyon, Almaty Region, Kazakhstan. The macroscopic and microscopic studies of the A. nivea herb were performed according to the methodology of the European pharmacopeia (EuPh) 2.8.23 “Microscopic examination of the medicinal plant raw material”. The macroscopic studies were performed using a magnifying glass and a MBS-9 binocular microscope, the microscopic studies were done using MS Microscopes 10 (oculars X5, X10, X15, lenses x10,x40), Micromed XS-4130 (oculars WF15X, lenses x40/0.65, x10/0.25) with a microphotonozzle (China). Identification of the main substances was carried out by the TLC method, testing and the quantitative determination of the flavonoid content were performed according to the EuPh methods.Results and discussion. Morphological and anatomical features of the A. nivea herb have been determined; on their basis Identifications A and B have been proposed; TLC Identification C of the main BAS of the raw material has been developed; indicators of purity tests have been determined. It has been proposed to carry out the quantitative determination by the content of flavonoids.Conclusions. The parameters of the A. nivea herb standardization have been determined on the basis of the following indicators: macroscopic and microscopic features, TLC identification of the main BAS of the raw material (hyperoside, rutin, quercetin and chlorogenic acid), related impurities (not more than 2 %), stems with a diameter of more than 20 mm (not more than 10 %), the loss on drying (not more than 13 %), the total ash (not more than 10 %) and at least 0.5 % flavonoids calculated with reference to rutin.

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“…The content of related impurities (2.8.2), the loss on drying (2.2.32), the total ash content (2.4.16) were determined according to the requirements of the EuPh [18, 23,24].…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

Standardization Parameters of Alfredia nivea KAR.&KIR Herb

Rustemkulov¹,

Махатова²,

Rustemkulova³

et al. 2023

J. Org. Pharm. Chem.

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“…50.0 mg (accurately weighed quantity) of the Arctostaphylos uva-ursi extracts were weighed in a 5.0 ml measuring tube and brought to the mark with 90 % aqueous methanol. After 30 minutes in an ultrasonic bath, the sample was insisted at room temperature for 3-4 h. Then the test tube was again placed on an ultrasound bath for 15 minutes, then the solution was filtered through a teflon filter with a pore size of 0.45 μm in vial for analysis [14,15]. Standard substances manufactured by Sigma-Aldrich USA (B9757, O5504, U6753, U6628, L5632 A4256, G7384, E2250, R5143, 83388, Y0001931, Q4951, 17793, C1251, PHL83773), were used for analysis.…”

Section: Methodsmentioning

confidence: 99%

“…The qualitative composition and quantitative content of saponins were studied by the method of highperformance liquid chromatography (HPLC) on a Shimadzu LC20 Prominence liquid chromatograph in the modular system equipped with the 4-channel pump LC20AD, the column thermostat СТО20А, the automatic sampler SIL20A, the diode array detector SPDM20A and Chem Station LC20 in the following chromatographic conditions: the X-Bridge C18 chromatographic column with the size of 150 × 4.6 mm and the particle size of 5 µm(Waters company); the column temperature -30 °С; the detection wavelength -205 nm; the flow rate of the mobile phase -1.0 ml/min; the injection volume -20 µl; the mobile phasemethanol for HPLC : 0.2 % solution of ammonium acetate (рН 6.75) in the ratio of 80:20; the elution modeisocratic. Identification of the components was carried out by the retention time and compliance of UV spectra with reference substances [14]. Triterpenic saponins was detected at 205 nm based on their absorption maximum at 200-210 nm.…”

Section: Methodsmentioning

confidence: 99%

“…The quantitative content of the basic groups of BAS in the Arctostaphylos uva-ursi extracts were determined by the method of absorption spectrophotometry on the spectrophotometer Evolution TM 60S UV-Visible (Thermo Fisher Scientific, USA) [18,19]. In all the Arctostaphylos uva-ursi extracts the extractive substances were determined gravimetrically [5] after complete drying at room temperature taking into account the loss on drying; the sum of the hydroxycinnamic acid derivates was determined by direct spectrophotometry (as chlorogenic acid, λ = 325 nm) [20,21]; flavonoids were quantified by the method of dierential spectrophotometry with aluminium chloride (as rutin, λ=410 nm) [4,19]; polyphenols were quantified by direct spectrophotometry (as gallic acid, λ=270 nm) according to Koshovyi et al [14]. All assays were performed in triplicate.…”

Section: Methodsmentioning

confidence: 99%

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Phytochemical profile and pharmacological activity of the dry extract from Arctostaphylos uva-ursi leaves modified with phenylalanine

Chaika

Коshovyi

Raal

et al. 2020

ScienceRise: Pharmaceutical Science

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“…Sage officinalis extracts have a high amino acid content, over 1 mg/g [39]. The main amino acids that are found in the leaves of Sage officinalis are alanine, glycine, glutamic acid, proline, and valine [39,40]. NADES based on lactic acid are very effective in extraction of polyphenol antioxidants from medicinal plants, with increased solubilizing capacity for flavonoids [41].…”

Section: Salvia Officinalismentioning

confidence: 99%

The Effect of Lactic Acid Fermentation on Extraction of Phenolics and Flavonoids from Sage Leaves

et al. 2022

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This work analysed the effect of spontaneous fermentation of sage leaves on the release and extraction of flavonoid and phenolic compounds. Chemical profiling based on thin-layer chromatography was used to compare different extracts from two sage species, common sage (Salvia officinalis) and white sage (Salvia apiana). Non-fermented Salvia apiana extracts are richer in antioxidants, phenolics, and terpenoids. Fermentation significantly enhances extraction of total phenolics, flavonoids, and antioxidants only from Salvia officinalis leaves, while it does not affect extraction from Salvia apiana leaves. In each 20 µL of extract, extraction of polyphenolics increases from 6.55 to 21.01 µg gallic acid equivalents (GAE), anti-oxidants from 0.68 to 2.12 µg GAE, and flavonoid content from 48.01 to 65.33 µg RE (rutin equivalents). Higher antioxidant activity in fermented Salvia apiana ethyl acetate extracts is associated with an increased concentration of phenolics and phenolic terpenoids. However, in Salvia officinalis, the higher antioxidant activity of fermented extract is a result of the release and improved extraction of flavonoids, as there is no increase in the extraction of phenolics. Lactic acid produced via fermentation and proline from Salvia officinalis leaves forms a natural deep eutectic solvent (NADES), which significantly increases the solubility of flavonoids.

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Phytochemical study of Salvia grandiflora and Salvia officinalis leaves for establishing prospects for use in medical and pharmaceutical practice

Cited by 5 publications

References 14 publications

Standardization Parameters of Alfredia nivea KAR.&KIR Herb

Standardization Parameters of Alfredia nivea KAR.&KIR Herb

Phytochemical profile and pharmacological activity of the dry extract from Arctostaphylos uva-ursi leaves modified with phenylalanine

The Effect of Lactic Acid Fermentation on Extraction of Phenolics and Flavonoids from Sage Leaves

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