Context: According to Ayurveda Acharyas for getting desirable outcome of any medication, it should be precisely analysed. Most of the Ayurveda classical formulations need to be standardized based on newer techniques for their Worldwide acceptance. Rajanyadi churna is one of the widely used formulation by Ayurveda physicians for all types of paediatric disorders mainly GI tract related conditions. Rajanyadi Churna is traditionally used in Churna form, in this study churna was converted into Syrup form for better palatability and convenience of administration. The present study mainly deals with the preparation and standardization of Rajanyadi syrup on the basis of organoleptic characteristics, physicochemical parameters and HPTLC fingerprinting.