Phytochemical screening, total polyphenol, flavonoid content, in vitro antioxidant and antibacterial activities of Sri Lankan varieties of Murraya koenigii and Micromelum minutum leaves

Abstract

The use of plant materials in traditional medicine as a significant ingredient in synthesizing drugs in the form of decoctions had played a vital role due to their potential therapeutic action. The curry leaves, Murraya koenigii, and Micromelum minutum are two most common aromatic herbs widespread in Sri Lanka and many other Asian countries. They are rich in nutrition and exert several medicinal properties such as antidiabetic, antioxidant, antimicrobial, anti-inflammatory, and anti-carcinogenic due to various essential phytochemicals, minerals, and trace minerals. In this study, we determined the proximate composition, in vitro antioxidant activity, total phenol (TPC), flavonoid content (TFC), and antibacterial activity in both species using standard methods. Results showed that both species varied greatly in nutritional composition, antioxidant, and antibacterial activities. The nutrient composition in terms of ash, crude protein, and fat was significantly high in M. koenigii based on dry weight basis. In vitro antioxidant potential of M. koenigii and M. minutum was evaluated by means of the DPPH radical scavenging assay, and the IC 50 values of M. koenigii and M. minutum were 107 AE 2 μg/mL and 208 AE 4 μg/mL, respectively. The TPC and TFC of M. koenigii were 101 AE 1 mg GAE/g and 9.75 AE 0.05 mg QE/g, and M. minutum were 80 AE 2 mg GAE/g and 9.16 AE 0.02 mg QE/g respectively. The antibacterial study was assessed against three bacterial strains. The study showed the highest inhibitory activity for M. koenigii and M. minutum against Staphylococcus aureus and Escherichia coli, respectively at 1.0 mg/mL concentration. The study indicated that M. koenigii possessed a significantly high TPC, and TFC correlated with higher antibacterial and antioxidant activity than M. minutum.

Quantitative Estimation of Immunomodulatory Flavonoid Quercetin by HPTLC in Different Leafy Vegetables Available in West Bengal

“…[27] The methanol extract of C. sativum L. had a TFC of 60.87 mg QE/g dry weight, whereas the methanolic extract of M. koenigii had a TFC of 9.75 mg QE/g dry weight, according to D.T. Abeysinghe et al [28] and Hasna Bouhenni et al [29] The flavonoid concentration of leafy vegetables was shown to be considerably influenced by biological, environmental, genetic, seasonal, and yearly fluctuations, according to publications in the literature.…”

Computational Analysis of Phytocompounds Present in Murraya Koenigii to Target Haemophilus Influenzae Disease

“…The top three ligands were downloaded in.SDF format from PubChem (https://pubchem. ncbi.nlm.nih.gov/) [8,11,12].…”

Nutritive Importance and Therapeutics Uses of Three Different Varieties (Murraya koenigii, Micromelum minutum, and Clausena indica) of Curry Leaves: An Updated Review

“…Various direct and indirect methods have assessed the antioxidant activities of the plants of the Rutaceae family. Most parts of M. koenigii are reported to be a rich source of plant polyphenols, flavonoids, and alkaloids, possessing a high antioxidant potency [ 98 – 100 ]. A recent study reported that total antioxidant activity was the highest in M. koenigii (2691 μ mol of ascorbic acid/g sample) among green leafy vegetables [ 101 ].…”