Phytochemical Screening, Spectroscopic & HPTLC Evaluation of Amalaki Formulation

Abstract

Amalaki, i.e., Indian gooseberry, is a medicinal plant with rejuvenator, anti-aging, and adaptogenic properties. It has a significant amountof ascorbic acid, an essential nutrient for various biochemical and physiological processes in the body. Amalaki is used in different ayurvedicformulations like churna, juice, rasayan, chyavanaprash, dhatri lauha, dhatryadi ghrita, and Triphala churna. Even so, more work was needed toestablish quality control standards for Amalaki formulations. This is because the plant is considered safe, and there are few reports of adverseeffects. Therefore, there needs to be more incentive to conduct rigorous studies on the safety and efficacy of Amalaki formulations. Despite thislack of research, Amalaki is still widely used with few reported side effects, making it an attractive option for those seeking natural remedies. In thepresent study, the phytochemical screening & physical evaluation were carried out of Amalaki formulations. The study includes determiningorganoleptic characters, moisture contents, ash, extractive values, etc. Marketed amalaki formulations were extracted in the microwave usingsolvent methanol: water (70:30). UV-Vis spectrophotometer was used for quantitative phytochemical evaluation of extracts of amalakiformulations. Alkaloids, flavonoids, carbohydrates, phenols, and tannins were found in the qualitative phytochemical evaluation of amalakiformulations. Total phenolic, flavonoid, tannin, sugar, and reducing sugar content of extract of Amalaki formulation AMK I & AMK II was found tobe 114.11±0.035mg GAE/g & 116.12 ± 0.020 mg GAE/g, 29.15 ± 0.0029 mg QE/g & 29.75 ± 0.0020 mg QE/g, 4.50 ± 0.0085 mg CE/g & 4.70 ±0.0079 mg CE/g, 7.34 ± 0.0034 mg Glu/g & 7.44 ± 0.0043 mg Glu/g & 3.41 ± 0.0035 mg Glu/g & 3.65 ± 0.0031 mg Glu/g, respectively. The FT-IRspectra of the extract of amalaki formulations were recorded in the region 4000–400 cm−1. They confirmed the presence of the O-H, aromatic CHstarch, C=C & C-O groups. The rutin, ascorbic acid, gallic acid, & kaempferol concentrations in the extracts of amalaki formulation wereeffectively estimated using the HPTLC method for marketed amalaki formulations.