Phytochemical screening of Tamarix dioica Roxb. ex Roch

Samejo, Muhammad Qasim; Sumbul, Adeela; Shah, Shahnila; Memon, Sara Bano; Chundrigar, Shahjabeen

doi:10.1016/j.jopr.2013.02.017

Cited by 26 publications

(38 citation statements)

References 3 publications

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“…The phytochemical assays were done as described previously with minor modifications [ 68 ]. To test the aqueous extracts for the presence of proteins, a few drops of 5.0% NaOH and a few drops of 1.0% Cu(SO 4 ) 2 were added to 2.0 mL of each aqueous extract.…”

Section: Methodsmentioning

confidence: 99%

Inhibition of Bacteria Associated with Wound Infection by Biocompatible Green Synthesized Gold Nanoparticles from South African Plant Extracts

et al. 2017

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Unlike conventional physical and chemical methods, the biogenic synthesis of gold nanoparticles (GNPs) is considered a green and non-toxic approach to produce biocompatible GNPs that can be utilized in various biomedical applications. This can be achieved by using plant-derived phytochemicals to reduce gold salt into GNPs. Several green synthesized GNPs have been shown to have antibacterial effects, which can be applied in wound dressings to prevent wound infections. Therefore, the aim of this study is to synthesize biogenic GNPs from the South African Galenia africana and Hypoxis hemerocallidea plants extracts and evaluate their antibacterial activity, using the Alamar blue assay, against bacterial strains that are known to cause wound infections. Additionally, we investigated the toxicity of the biogenic GNPs to non-cancerous human fibroblast cells (KMST-6) using 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) assay. In this paper, spherical GNPs, with particle sizes ranging from 9 to 27 nm, were synthesized and fully characterized. The GNPs from H. hemerocallidea exhibited antibacterial activity against all the tested bacterial strains, whereas GNPs produced from G. africana only exhibited antibacterial activity against Pseudomonas aeruginosa. The GNPs did not show any significant toxicity towards KMST-6 cells, which may suggest that these nanoparticles can be safely applied in wound dressings.

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Section: Methodsmentioning

confidence: 99%

Inhibition of Bacteria Associated with Wound Infection by Biocompatible Green Synthesized Gold Nanoparticles from South African Plant Extracts

et al. 2017

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“…Phytochemical studies confirmed the occurrence of flavonoids, phenols, proteins, amino acids, saponins, tannins, terpenoids, and fixed oils in aqueous extracts of various parts of the plant, as well as pholbatannins and steroids [2]. Spectroscopic analysis revealed the isolation of many flavonoids from T. dioica (aerial parts), like tamarexetin, tameridone, tamadone with hexacosyl pcoumarate, nevadensin A and apigenin, kaempferide, quercetin, and gardenins A, B, C, and E [17,18].…”

Section: Introductionmentioning

confidence: 99%

“…Tamarix dioica Roxb. ex Roth (Tamaricaceae) is locally known by various vernacular names like ghaz, jhao, khagal, lai, pilchi, and Ukkan in Pakistan [2,3]. Tamarix dioica is a perennial plant with the ability to tolerate changes in climate.…”

Section: Introductionmentioning

confidence: 99%

The Potential Involvement of an ATP-Dependent Potassium Channel-Opening Mechanism in the Smooth Muscle Relaxant Properties of Tamarix dioica Roxb.

et al. 2019

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Background: Tamarix dioica is traditionally used to manage various disorders related to smooth muscle in the gastrointestinal, respiratory, and cardiovascular systems. This study was planned to establish a pharmacological basis for the uses of Tamarix dioica in certain medical conditions related to the digestive, respiratory, and cardiovascular systems, and to explore the underlying mechanisms. Methods: A phytochemical study was performed by preliminary methods, followed by HPLC-DAD and spectrometric methods. In vivo evaluation of a crude hydromethanolic extract of T. dioica (TdCr) was done with a castor-oil-provoked diarrheal model in rats to determine its antidiarrheal effect. Ex vivo experiments were done by using isolated tissues to determine the effects on smooth and cardiac muscles and explore the possible mechanisms. Results: TdCr tested positive for flavonoids, saponins, phenols, and tannins as methanolic solvable constituents in a preliminary study. The maximum quantity of gallic acid equivalent (GAE), phenolic, and quercetin equivalent (QE) flavonoid content found was 146 ± 0.001 μg GAE/mg extract and 36.17 ± 2.35 μg QE/mg extract. Quantification based on HPLC-DAD (reverse phase) exposed the presence of rutin at the highest concentration, followed by catechin, gallic acid, myricetin, kaempferol, and apigenin in TdCr. In vivo experiments showed the significant antidiarrheal effect of TdCr (100, 200, and 400 mg/kg) in the diarrheal (castor-oil-provoked) model. Ex vivo experiments revealed spasmolytic, bronchodilatory, and vasorelaxant activities as well as partial cardiac depressant activity, which may be potentiated by a potassium channel opener mechanism, similar to that of cromakalim. The potassium channel (KATP channel)-opening activity was further confirmed by repeating the experiments in glibenclamide-pretreated tissues. Conclusions: In vivo and ex vivo studies of T. dioica provided evidence of the antidiarrheal, spasmolytic, bronchodilator, vasorelaxant, and partial cardiodepressant properties facilitated through the opening of the KATP channel.

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“…Phytochemical screening of okra seed extract, ethanol, and dichloromethane and petroleum fraction of okra seeds were performed for the qualitative detection of different phytochemicals such as alkaloids, flavonoids, saponins, glycosides, phenols, and tannin. All phytochemical tests were performed according to the standed methods described by Samejo et al [16] and Maria et al [17].…”

Section: Methodsmentioning

confidence: 99%

The Effect of Okra (Abelmoschus esculentus (L.) Moench) Seed Extract on Human Cancer Cell Lines Delivered in Its Native Form and Loaded in Polymeric Micelles

Chaemsawang

Prasongchean

Papadopoulos

et al. 2019

International Journal of Biomaterials

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Cancer is a noncommunicable disease with a high worldwide incidence and mortality rate. The National Cancer Institute of Thailand reports increasing cumulative incidence of breast, colorectal, liver, lung, and cervical cancers, accounting for more than 60% of all cancers in the kingdom. In this current work, we attempt to elucidate the phytochemical composition of the okra (Abelmoschus esculentus (L.) Moench) seed extract (OSE) and study its anticancer activity, delivered in its native form as well as in the form of polymeric micelles with enhanced solubility, in three carcinoma cell lines (MCF-7, HeLa, and HepG2). The presence of flavonoid compounds in the OSE was successfully confirmed, and direct delivery had the highest cytotoxic effect on the breast cancer cell line (MCF-7), followed by the hepatocellular carcinoma (HepG2) and cervical carcinoma (HeLa) cell lines in that order, while its delivery in polymeric micelles further increased this effect only in the HepG2 cell line. The OSE's observed cytotoxic effects on cancer cell lines demonstrated a dose and time-dependent cell proliferation and migration inhibition plausibly due to VEGF production inhibition, leading to apoptosis and cell death, conceivably due to the four flavonoid compounds noted in the current study, one of which was isoquercitrin. However, in view of the latter compound's isolated effects being inferior to those observed by the OSE, we hypothesize that either isoquercitrin requires the biological synergy of any one or all of the observed flavonoids or any of the three in isolation or all in concert are responsible. Further studies are required to elucidate the nature of the three unknown compounds. Furthermore, as we encountered significant problems in dissolving the okra seed extract and creating the polymeric micelles, further studies are needed to devise a clinically beneficial delivery and targeting system.

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Phytochemical screening of Tamarix dioica Roxb. ex Roch

Cited by 26 publications

References 3 publications

Inhibition of Bacteria Associated with Wound Infection by Biocompatible Green Synthesized Gold Nanoparticles from South African Plant Extracts

Inhibition of Bacteria Associated with Wound Infection by Biocompatible Green Synthesized Gold Nanoparticles from South African Plant Extracts

The Potential Involvement of an ATP-Dependent Potassium Channel-Opening Mechanism in the Smooth Muscle Relaxant Properties of Tamarix dioica Roxb.

The Effect of Okra (Abelmoschus esculentus (L.) Moench) Seed Extract on Human Cancer Cell Lines Delivered in Its Native Form and Loaded in Polymeric Micelles

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