Phytochemical Screening of Punica granatum Peels Powder and Hemato-Biochemical Assessment of Induced E. coli Infection in Kaveri Birds Treated with Punica granatum Peels Powder and Antibiotic (Doxcycline + Neomycin)

Abstract

The present experimental trial was conducted to assess the effect of Punica granatum peel powder (PPP) and antibiotic (Doxycycline + Neomycin) against induced E.coli infection in Kaveri birds. 120 day old Kaveri birds were randomly divided into 6 groups (T0, T1, T2, T3, T4 and T5). The Kaveri birds in Group-T0 were kept as healthy control. At the age of 8th day, Group-T1 birds were given oral dose of E. coli broth containing 0.3-0.5ml (1x109CFU/ml), Group-T2 were given broth of E. coli @ 0.3-0.5ml and PPP @ 1% of feed daily for 29 days, Group-T3 were given broth of E. coli @ 0.3-0.5ml and antibiotic @ 1gm/10 kg B.W through drinking water for 7 days. Group-T4 were given broth of E. coli @ 0.3-0.5ml and antibiotic @ 1gm/10kg B.W for 7 days through water and PPP @ 1% of feed for 7 days while, Group-T5 were given PPP @ 1% of feed daily. At day 0, 7th, 14th and 28th day intervals, haematobiochemical parameters were recorded at weekly intervals. The E.coli infected Kaveri birds showed significant alterations in haematobiochemical parameter at 7th and 14th day post infection intervals of trial. The treatment of PPP through feed against induced E.coli infection given in Kaveri birds played partial protective role. The antibiotic (Doxycycline + Neomycin) treatment found to be efficacious against induced E.coli infection at 7th and 14th post infection interval. The combination of antibiotic through water with PPP through feed had significant efficacious role than individual treatment at 7th and 14th post infection interval.