Phytochemical screening of extracts from Spiranthera odoratissima A. St.-Hil. (Rutaceae) leaves and their in vitro antioxidant and anti-Listeria monocytogenes activities

Abstract

Spiranthera odoratissima A. St.-Hil (Rutaceae), a shrub whose common name is manacá do Cerrado in Brazilian Portuguese, is about 1-m high and has been used by folk medicine to treat stomachache, kidney and liver infections, headache, rheumatism and as a blood purifier. This study aimed at preparing hexane, ethyl acetate, methanolic, hydroethanolic and aqueous extracts from S. odoratissima leaves, at carrying out preliminary phytochemical screening and at evaluating their in vitro antioxidant and anti-Listeria monocytogenes activities. Antioxidant activity was evaluated by the DPPH (2,2-diphenyl-1-picrylhydrazyl), ABTS (2,2-azinobis-3-ethybenzothiazoline-6-sulfonate) and FRAP (ferric reducing antioxidant power) methods. Antibacterial activity was investigated against L. monocytogenes and Minimum Inhibitory Concentration (MIC) values of plant extracts were calculated by the broth microdilution method with the use of 96-well plates. In aqueous, methanolic, hydroethanolic, ethyl acetate and hexane extracts from S. odoratissima leaves, the following classes of compounds were investigated: organic acids, reducing sugars, flavonoids, saponin compounds, coumarin compounds, phenolics, tannins, purine compounds, catechins, flavonol derivatives, sesquiterpene lactones and anthraquinones. All plant extracts, except the hexane one, exhibited high antioxidant activity. Regarding antibacterial activity, the most polar extracts showed high activity against L. monocytogenes; their MIC values ranged between 12.5 and 62.5 µg mL-1, while the hexane one exhibited low activity (MIC = 1000 µg mL-1). In short, extracts from S. odoratissima leaves may be considered promising sources of secondary metabolites with relevant antioxidant and antibacterial activities.

Antifungal Activity of Plantago lanceolata and Sida ovata Leaf Extracts against Dermatomycotic Fungi

“…The above finding was in line with the research report by Irobi et al [ 17 ] whose analysis of this plant showed the presence of carbohydrates, alkaloids, saponins, fixed oil, and phytosterols. The above finding was still in concurrent with the study report carried out by Souza et al [ 18 ] whose analysis of Spiranthera odoratissima showed the presence of organic acids, reducing sugars, flavonoids, saponin compounds, coumarin compounds, phenolics, tannins, purine compounds, catechins, flavonol derivatives, sesquiterpene lactones, and anthraquinones. This shows that the phytochemicals from the studied plants leaves extracts had contributed to their antimicrobial activity against the tested clinically isolated dermatomycotic fungi.…”

Identificação e avaliação da atividade antimicrobiana de óleorresina de pimentas do gênero Capsicum spp. frente ao Staphylococcus aureus

“…Os óleos essenciais ou óleorresinas são considerados ativos biológicos que apresentam compostos com atividades antimicrobianas (Cui et al, 2019;Cui et al, 2020;Souza et al, 2020). Controlar microrganismos patogênicos, como as bactérias, que são contaminantes indesejáveis que afetam diretamente os humanos é uma necessidade de saúde pública (DRUMOND et al, 2018).…”

Cytogenotoxic potential of Malva parviflora L. cultivated under different light conditions

“…Medicinal plants are less or non-toxic drug resources that are easily accessible, economical, and safe (Fathi, Ghane, & Pishkar, 2022). For many centuries, the treatment with these plants was the only resource available to various ethnic groups, and currently, plants continue to be frequently used for the treatment of pathologies in many countries (Gasparetto, Martins, Hayashi, Otuky, & Pontarolo, 2011;Souza et al, 2020), including Brazil. Besides being part of the culture, their use can be influenced by the high cost of medicines, the difficulty of access to medical consultations (Oliveira, Bovini, Bortoluzzi, Boff, & Boff, 2019), and the growing trend of the return of the use of natural resources as an alternative to synthetic medicines (Liu, Wang, & Kang, 2022).…”