Phytochemical Screening, HPTLC Fingerprinting and Invitro Antioxidant Activity of Root Extract of Asparagus racemosus

Abstract

Background: Asparagus racemosus is a climber shrub used in Indian medicine for centuries. It has been used as galactogogue and nerve tonic in folk medicine. The recent research on A. racemosus has revealed its disease fighting properties such as anti-bacterial, immunomodulatory, cardio protective, anti-stress, etc. Phytochemicals present in the plants are associated with their therapeutic capabilities. Hence, phytochemical screening of a therapeutic plant is essential. Materials and Methods: A preliminary qualitative screening of phytoconstituents present in the ethanol and aqueous extract of the plant was done. high-performance thin layer chromatography (HPTLC) was used to create a phytochemical fingerprint of the plant extract. Further, a series of antioxidant assays, i.e., 2,2-diphenyl-1picrylhydrazyl (DDPH) radical, Nitric oxide (NO) radical, Superoxide (SO) radical and 2,2'-azinobis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radical scavenging assays were done. Results: The phytochemical screening and the HPTLC fingerprint showed the presence of phenolic compounds, flavonoids, glycosides, triterpenoids, saponins etc. They also showed free radical scavenging property and hence can be used as potential primary antioxidant. Conclusion: A preliminary screening created a phytochemical profile of A. racemosus extracts. These phytoconstituents may be linked to the various known therapeutic applications of the plant. This may aid in further extensive studies for identifying and isolating compounds with potential therapeutic value in A. racemosus.

In Vitro and In Vivo Regulation of SRD5A mRNA Expression of Supercritical Carbon Dioxide Extract from Asparagus racemosus Willd. Root as Anti-Sebum and Pore-Minimizing Active Ingredients

“…In addition, previous reports showed the presence of polyphenolic compounds from the aerial parts of A. racemosus [ 29 , 36 ]. Interestingly, steroidal saponins, polysaccharides, glycosides, sterols, and triterpenoids were also found in the roots of A. racemosus [ 24 , 27 , 29 ]. Novel steroidal saponins, such as shatavarins I-V, shatavarosides A and B, asparinins, asparosides, curillins, and curillosides, have previously been isolated from a root of Shatavari by acetonitrile or methanolic extraction [ 31 , 32 , 33 ], and they could be found in the A. racemosus extract from our study.…”

“…In Ayurveda, A. racemosus is recommended as an immunoadjuvant and lactation booster [ 17 ]. Moreover, most studies have reported various biological activities of A. racemosus extract, such as immunomodulatory [ 18 ], galactagogue [ 19 ], antibacterial [ 20 , 21 ], antifungal [ 22 ], and antioxidant [ 23 , 24 ] properties. Additionally, A. racemosus extract has profound antibacterial and antifungal activities against Staphylococcus aureus [ 20 , 21 ] and Malassezia furfur [ 22 ], which are related to inflammatory skin diseases, such as seborrheic dermatitis [ 25 , 26 ].…”

Regulation of Insulin Resistance, Lipid Profile and Glucose Metabolism Associated with Polycystic Ovary Syndrome by Tinospora cordifolia

“…Then, the filtrate was evaporated using a water bath and rotary evaporator at 60–70 °C. After evaporation, the hydroalcoholic extract was completely dry and ready for use [ 18 ].…”

“…After seven days, the mixture was filtered through a Buchner funnel to create a clear filtrate. Then, the filtrate was evaporated using a water bath and rotary evaporator at 60-70 • C. After evaporation, the hydroalcoholic extract was completely dry and ready for use [18].…”

Spectroscopic and chromatographic identification of bioprospecting bioactive compounds from cow feces: Antimicrobial and antioxidant activities evaluation of gut bacterium Pseudomonas aeruginosa KD155

“…Approximately 10 μl of samples and the standard solution was used for the spotting. After saturation time, the spots were analyzed in UV spectra for the similarity of peaks and retardation factor (Rf) value [ 43 ].…”