Phytochemical Screening, Antioxidant Effect and Down Regulation of TGF-β Induced by Ophiocoma erinaceus Brittle Star Crude Extract

Baharara, Javad; Amini, Elaheh

doi:10.17795/zjrms-5194

Cited by 7 publications

(10 citation statements)

References 20 publications

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“…The down-regulation of VEGF and bFGF was stronger than the down-regulation of TGF-β. This finding, at gene expression levels, confirms the Baharar's study showing the anti-angiogenesis effects of a brittle star extract in a CAM model [22].…”

Section: Discussionsupporting

confidence: 88%

“…Several studies have investigated Ophiuroids as a source of anti-tumor and anti-metastatic organisms. Considerable number of studies are indicative of the noticeable anti-tumor properties of the Ophiuroids [21,22,23]. The Persian Gulf as a rich marine ecosystem is a territory of different species of Ophiuroids containing components characteristics of this part of the world.…”

Section: Introductionmentioning

confidence: 99%

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Down-regulation of TGF-β, VEGF, and bFGF in vascular endothelial cells of chicken induced by a brittle star (Ophiocoma erinaceus) extract

Kachooei

Rahmani

Zareh

et al. 2020

Heliyon

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A B S T R A C TGreat attention has been focused on the discovery of anti-angiogenic natural and synthetic compounds to be finally used as or at least a part of the treatment of tumors. The marine ecosystems provide diversity in natural chemicals with the potential of being exploited as medicines in the treatment of diseases. Several studies have investigated Ophiuroids as a source of anti-tumor and anti-metastatic organisms. Here, we described the inhibitory effects of an ethanolic crude extract of brittle star (Ophiocoma erinaceus) on angiogenesis and the expression level of TGF-β, VEGF, and bFGF in chicken chorioallantoic membrane (CAM) as an experimental model. To do this 45 embryonated eggs were randomly divided into six groups including the control group, sham, three experimental groups and positive. The number and the length of vessels were calculated using ImageJ ® software. The relative mRNA levels of the genes in different groups were evaluated by qRT-PCR method. Our study was suggestive of an anti-angiogenesis effect of brittle star ethanolic crude extract in a CAM model. The extract also showed a pharmacological effect of down-regulation of mRNA related to VEGF, TGF-β, and bFGF genes on chicken vascular endothelial cells. It was also showed that the observed inhibitory effect is with a dose-dependent manner in which the highest inhibitory effect belonged to the highest used dose. We indicated the anti-angiogenesis properties of the Persian Gulf brittle star. Further studies are needed in other aspects of the brittle star extract in the treatment of angiogenesis, hyperplasia, and cancers.

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Section: Discussionsupporting

confidence: 88%

Section: Introductionmentioning

confidence: 99%

Down-regulation of TGF-β, VEGF, and bFGF in vascular endothelial cells of chicken induced by a brittle star (Ophiocoma erinaceus) extract

Kachooei

Rahmani

Zareh

et al. 2020

Heliyon

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“…Therefore, the proliferation of cancer cells in metastatic state may become faster as a result of freeradical-mediated injuries (Nishikawa and Hashida, 2006). Our previous observations exhibited that brittle star methanol extract has cytotoxic and antioxidant ability (Baharara and Amini, 2015). In this study, the antioxidant capacity of isolated saponin has been confirmed by DPPH and ABTS methods.…”

Section: Discussionsupporting

confidence: 55%

“…Arm regeneration is prominent feature of brittle star that make it popular as an experimental study (Czarkwiani et al, 2013). In the preliminary study we found cytotoxic, antioxidant and hemolytic activity of brittle star (Baharara and Amini, 2015). However, it has been reported that saponins possess wound healing, cytotoxic and hemolytic effect, we decided to extract saponin from brittle star and evaluatetherapeutic effect of extracted saponin.…”

mentioning

confidence: 92%

Metastatic Inhibitory and Radical Scavenging Efficacies of Saponins Extracted from the Brittle Star (Ophiocoma erinaceus)

Amini¹,

Nabiuni²,

Baharara³

et al. 2015

Asian Pacific Journal of Cancer Prevention

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“…The growth factors subsequently bind to their corresponding receptor tyrosine kinases (RTKs) within the endothelial cells in blood vessels (6,7). This receptor binding results in the activation of the otherwise dormant endothelial cells, causing to divide and migrate towards the diseased tissues, or, in this case, the tumor cells (8).…”

Section: Introductionmentioning

confidence: 99%

Anti-Vasculogenic Activity of a Polysaccharide Derived from Brittle Star via Inhibition of VEGF, Paxillin and MMP-9

Baharara

Amini

Musavi

2017

Iran. J. Biotechnol.

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Background: Bioactive compounds such as terpenoids, chondroitin sulfate, and polysaccharides with added value can be found in prestine marine creatures. These compounds often do have highly valuable therapeutic applications such as being antioxidant, antitumorogenic, anti-infl ammatory and anti-angiogenic. For the latter, varieties of angiogenesis factors can suppress this issue within the bodily tissues. Objectives: The anti-angiogenic and anti-metastatic capacity of a polysaccharide derived from brittle star was investigated. Material and Methods:The anti-proliferative eff ect of derived polysaccharide on umbilical vein endothelial cells (HUVEC) was measured using MTT (dimethyl thiazol-2-yl]-2,5-diphenyl tetrazolium bromide) assay. The anti-angiogenic eff ect of the isolated polysaccharide was examined by Chorioallantoic membrane (CAM) assay. The transcriptional expression of VEGF (Vascular Endothelial Growth Factor) was evaluated by Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR). The anti-metastatic activity was investigated via scratch-wound healing assay. The levels of Paxillin and Matrix Metalloproteinase-9 (MMP-9) expression were analyzed by RT-PCR. Statistical analysis and mean comparisons (p< 0.05) were carried out by SPSS 16. Results: Our results elucidated that the brittle star isolated polysaccharide exerted a dose dependent cytotoxic eff ect on the HUVEC endothelial cells. The CAM assay exhibited potent anti-angiogenic activity in vivo. The RT-PCR analysis showed that the extracted polysaccharide (40, 60 μg.mL ) was administrated via down-regulation of paxillin and MMP-9 mRNA expression. Conclusions: Taken together, these results indicated that the polysaccharide extracted from brittle star was able to decrease the viability of the HUVEC cells, to suppress angiogenesis, and possibly act as a natural anti-angiogenic and anti-metastatic marine organic compound against angiogenesis related pathologies.

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Phytochemical Screening, Antioxidant Effect and Down Regulation of TGF-β Induced by Ophiocoma erinaceus Brittle Star Crude Extract

Cited by 7 publications

References 20 publications

Down-regulation of TGF-β, VEGF, and bFGF in vascular endothelial cells of chicken induced by a brittle star (Ophiocoma erinaceus) extract

Down-regulation of TGF-β, VEGF, and bFGF in vascular endothelial cells of chicken induced by a brittle star (Ophiocoma erinaceus) extract

Metastatic Inhibitory and Radical Scavenging Efficacies of Saponins Extracted from the Brittle Star (Ophiocoma erinaceus)

Anti-Vasculogenic Activity of a Polysaccharide Derived from Brittle Star via Inhibition of VEGF, Paxillin and MMP-9

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