Phytochemical screening, antioxidant and antimutagenic activities of selected Thai edible plant extracts

Phadungkit, Methin; Somdee, Thidarat; Kangsadalampai, Kaew

doi:10.5897/jmpr11.517

Cited by 24 publications

(24 citation statements)

References 22 publications

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“…The results showed that the extract had a moderate to strong inhibitory effect on mutagenesis induced by Trp‐P‐1, and the inhibitory activity increased with increasing concentrations of the extract. This should be correlated to its chemical compounds as it is composed of phenolic compounds having antioxidant activity that can inhibit mutation and cancer because they can serve as oxidation terminators by scavenging free radicals or inducing antioxidant enzymes (PhadungkitSomdee & Kangsadalampai, ). Orhan et al, reported that polyphenols were found to display potent antioxidant activity free radicals.…”

Section: Discussionmentioning

confidence: 99%

The chemical composition, antifungal, antioxidant and antimutagenicity properties of bioactive compounds from fungal endophytes associated with Thai orchids

Bungtongdee

Sopalun

Laosripaiboon

et al. 2018

Journal of Phytopathology

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Some plant‐derived bioactive compounds produced by fungal endophytes have been proven to have antimicrobial and antioxidant activities. In this study, endophytic fungi were isolated from 20 orchid samples collected in northern Thailand from 12 genera of orchids. In total, 97 isolates were isolated from the leaves (44.3%), stems (40.2%) and flowers (15.5%) of the orchid samples. The antifungal activity was investigated of the endophytic isolates against the plant pathogenic fungi. The results showed that 13 endophytic isolates provided antifungal activities against Fusarium sp., Colletotrichum sp. and Curvularia sp. The endophyte CK F05‐5, which was isolated from the flower part of Dendrobium lindleyi, was chosen for further testing because it the highest level of antifungal activity against Fusarium sp. The isolate CK F05‐5 was identified as Fusarium oxysporum on the basis of its ITS sequences of 5.8 s rRNA, and phytochemical analysis revealed the presence of coumarins. The ethyl acetate extract of CK F05‐5 was examined for its total phenolic content and antioxidant activity using Folin–Ciocalteu's reagent and 2,2‐diphenyl‐1‐picrylhydrazyl (DPPH) radical scavenging assay, respectively. The phenolic content was 160.51 mg of GAE/g of extract, and the free radical scavenging activity was 89.61 µg/ml at the half maximal inhibitory concentration (IC50). The antimutagenic potential of the ethyl acetate extract of CK F05‐5 against Trp‐P‐1 mutagenic substances was determined using the Ames test which revealed that the extract of CK F05‐5 at 10 mg/plate had the highest antimutagenic activity against Trp‐P‐1 (51.2%) and 39.6% for strains TA98 and TA100, respectively. The active compounds present in the acetate extract of CK F05‐5 were examined using GC‐MS analysis, which displayed the presence of gibepyrone A, pyrrolo [1, 2‐a] pyrazine‐1, 4‐dione, hexahydro‐3‐(2‐methylpropyl) and indoleacetic acid as major components. Based on the results, this endophytic fungus contains various bioactive components that have various biological activities. This useful information could help in producing potentially valuable and novel pharmaceutical products.

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Section: Discussionmentioning

confidence: 99%

The chemical composition, antifungal, antioxidant and antimutagenicity properties of bioactive compounds from fungal endophytes associated with Thai orchids

Bungtongdee

Sopalun

Laosripaiboon

et al. 2018

Journal of Phytopathology

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“…An antimutagen can inhibit the transformation of a compound into potential mutagen, inactivate it, prevent the interaction between mutagen and DNA or may modulate the enzymes of the DNA replication, repair and recombination pathways (Ferguson 1994;Bhattacharya 2011). Several natural and dietary components are reported to be antimutagens and potential cancer-chemopreventive agents, which can prevent or delay the onset or development of cancer (Edenharder et al 1995;Mitscher et al 1996;Phadungkit et al 2012). Cancer chemopreventives can act at the genetic level (in terms of both maintaining genomic integrity and modulating gene expression), thereby decreasing the disease incidence, or alternatively can hinder the progression of the disease by controlling cell proliferation, apoptosis, differentiation, angiogenesis and metastasis (Duvoix et al 2005).…”

Section: Introductionmentioning

confidence: 98%

Variety-based variation in the antimutagenic potential of various vegetables and lack of its correlation with their antioxidant capacity

Bandyopadhyay

Gautam

Sharma

2013

International Journal of Food Sciences and Nutrition

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As mutation causes many life-threatening diseases including cancer, a diet enriched with specific vegetables having potential to reduce mutagenesis possesses immense significance. In this study, 41 commonly used vegetables from diverse botanical taxa were evaluated and compared for their relative antimutagenic potential using standard assays [Escherichia coli RNA polymerase β (rpoB)-based Rif(S) → Rif(R) assay and Ames test] against known mutagens (UV, gamma radiation, 4-nitroquinoline-N-oxide and ethylmethanesulphonate). Significant differences in antimutagenicity were observed even among the cultivars within the same species, as well as at other phylogenetic levels such as genus or family. The effect of cooking in terms of boiling (aquathermal treatment), on the antimutagenicity of these vegetables, was also investigated. In majority of the cases, aquathermal treatment did not affect the antimutagenic potential. The antimutagenicity of these vegetables was not found to correlate well with their antioxidant properties.

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“…Cells at log phase of their growth cycle were treated in triplicate at various concentrations of the test samples (0.032-20.0 g/mL), and incubated for 72 h at 37°C in a humidified atmosphere of 5% CO2. Cell concentrations were determined by the sulforhodamine B method, this colorimetric method expresses the results in grown cell percentage in relation with the control using the following formula, % grown cell = (At-Ab)/(Ac-Ab) x100, where, At= absorbance value of test compound, Ab= absorbance value of blank, Ac=absorbance value of control (Skehan et al, 1990;Philip et al, 1990;Patel and Patel, 2010;Sivacumar and Alagesaboopathi, 2008;Phadungkit et al, 2010).…”

Section: Methodsmentioning

confidence: 99%

Cytotoxic evaluation of fluvastatin and rosuvastatin and effect of fluvastatin in the hela cell cycle

Campos-Lara¹,

Mendoza‐Espinoza²

2011

Afr. J. Pharm. Pharmacol.

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3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase inhibitors, were originally designed to reduce cholesterol biosynthesis and have been extensively used as prevention drugs against hyperlipidemia and cardiovascular conditions. Recently, these compounds have been shown to display chemopreventive activity against cancer. However, the effects of statins on cancer are not completely understood. For this reason, we have studied the cytotoxic effect of rosuvastatin and fluvastatin on three cell tumoral lines: human larynx carcinoma (HEp-2), human nasopharyngeal carcinoma (KB), and human epithelial carcinoma (HeLa). We have found that only fluvastatin has relevant activity against the tumoral cell lines assayed and the capacity to arrest G 1 -phase, whereas a significant decline was observed in the S-phase percentage. Fluvastatin IC 50 were 2.43±0.56 µ µ µ µg/mL (HEp-2), 2.29±0.19 µ µ µ µg/mL (KB), and 5.02±1.52 µ µ µ µg/mL (HeLa), whereas rosuvastatin showed poor activity. These results indicate that the cytotoxic effect of fluvastatin may not depend directly on HMG-CoA reductase inhibition. The antitumor statins effect needs further investigation.

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Phytochemical screening, antioxidant and antimutagenic activities of selected Thai edible plant extracts

Cited by 24 publications

References 22 publications

The chemical composition, antifungal, antioxidant and antimutagenicity properties of bioactive compounds from fungal endophytes associated with Thai orchids

The chemical composition, antifungal, antioxidant and antimutagenicity properties of bioactive compounds from fungal endophytes associated with Thai orchids

Variety-based variation in the antimutagenic potential of various vegetables and lack of its correlation with their antioxidant capacity

Cytotoxic evaluation of fluvastatin and rosuvastatin and effect of fluvastatin in the hela cell cycle

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