Phytochemical Screening, Antioxidant, and Antimicrobial Activities of Seven Underinvestigated Medicinal Plants against Microbial Pathogens

Bisso, Borel Ndezo; Nkwelle, Roland; Tchuenteu, Roland T.; Dzoyem, Jean Paul

doi:10.1155/2022/1998808

Cited by 18 publications

(9 citation statements)

References 41 publications

(45 reference statements)

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“…The extracts were screened for steroids, and they were found in all the extracts. Steroids derived from medicinal plants are known to possess antibacterial and insecticidal properties [ 30 ].…”

Section: Resultsmentioning

confidence: 99%

Assessing the Efficiency of Antimicrobial Plant Extracts from Artemisia afra and Eucalyptus globulus as Coatings for Textiles

Nortjie,

Basitere,

Moyo

et al. 2024

Plants

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This study aimed to assess the antimicrobial activities of plant extracts from Artemisia afra and Eucalyptus globulus when used as coatings for textiles. A pulsed ultrasound-assisted extraction method (PUAE) was employed to obtain methanolic and hexanoic extracts from both plants. Eucalyptus globulus methanol extraction exhibited the highest yield at 22.76% (±0.61%), while Artemisia afra demonstrated lower yields. Phytochemical screening identified various secondary metabolites in the extracts, including phenols, quinones, and steroids. Antimicrobial tests against Staphylococcus aureus and Escherichia coli revealed varying degrees of susceptibility, with Eucalyptus globulus hexanoic extracts showing the highest activity against Staphylococcus aureus at an average percentage growth of 18.74% (±0.26%). Minimum inhibitory concentration (MIC) values were determined for the extracts, but complete inhibition did not occur at concentrations below 500 μg/mL. The extracts exhibited varying effects on Staphylococcus aureus and Escherichia coli growth, with some extracts promoting bacterial growth. Coating textiles with Eucalyptus globulus methanolic extracts demonstrated antibacterial activity against Staphylococcus aureus with the highest zone of inhibition observed in cotton-coated samples (258.4 mm2). Polyester-coated samples exhibited smaller inhibition zones, with the lowest observed in Eucalyptus globulus methanolic extract coating (65.97 mm2). Scanning electron microscope (SEM) analysis revealed visible surface morphology changes in coated fabrics, depicting fine, cluster, lumpy, flaky, and fragment-like morphologies. Laundering effects on coated fabrics were investigated, showing a significant decrease in antimicrobial activity after washing. Fourier-transform infrared spectroscopy (FTIR) identified functional groups in the extracts associated with antimicrobial properties. The complexity of the bioactive compounds suggests potential antimicrobial efficacy, resting on factors such as geographical location, climate, and extraction methods. Notwithstanding the limitations, this study contributes valuable insights into the use of plant extracts as antimicrobial coatings for textiles.

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Section: Resultsmentioning

confidence: 99%

Assessing the Efficiency of Antimicrobial Plant Extracts from Artemisia afra and Eucalyptus globulus as Coatings for Textiles

Nortjie,

Basitere,

Moyo

et al. 2024

Plants

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“…In recent years, the emergence of antifungal drug resistance in C. neoformans has increased rapidly [27]. Hence, the morbidity and mortality rates due to cryptococcosis caused by C. neoformans are increasing continuously.…”

Section: Discussionmentioning

confidence: 99%

Biofilm Formation and Phospholipase and Proteinase Production in Cryptococcus neoformans Clinical Isolates and Susceptibility towards Some Bioactive Natural Products

Bisso

Makuété

Tsopmene

et al. 2023

The Scientific World Journal

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Background. Cryptococcosis is one of the most common fungal infections in immunocompromised patients, which is caused by Cryptococcus neoformans. However, relatively little is known about the virulence factors of C. neoformans and the incidence of antifungal drug resistance in C. neoformans is rapidly increasing. This study was undertaken to investigate the virulence factors in C. neoformans, thymol, curcumin, piperine, gallic acid, eugenol, and plumbagin for their potential antimicrobial activity against C. neoformans. Methods. The production of phospholipase and proteinase was detected using standard methods. Biofilm formation was determined using the microtiter plate method. The broth microdilution method was used to determine the antifungal activity. The antibiofilm activity was assessed using the safranin staining method. Results. All isolates of C. neoformans produced biofilms with optical density values ranging from 0.16 to 0.89. A majority of C. neoformans isolates that were tested exhibited strong phospholipase (7/8) and proteinase (5/8) production. Plumbagin (with minimum inhibitory concentration values ranging from 4 to 16 μg/mL) showed the highest antifungal activity followed by thymol (with minimum biofilm inhibitory concentration values ranging from 8 to 64 μg/mL). In addition, plumbagin showed the highest antibiofilm activity with minimum biofilm inhibitory concentration and minimum biofilm eradication concentration values ranging from 4 to 16 μg/mL and 32 to 256 μg/mL, respectively. Conclusion. Plumbagin, compared to other natural products studied, was the most efficient in terms of antifungal and antibiofilm activities. Hence, plumbagin could be used in combination with antifungals for the development of new anticryptococcal drugs.

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“…There is a shift of focus to medicinal plants due to the increased antimicrobial resistance, severe adverse effects, high costs of synthetically available drugs and the community perception towards modern health care practice [33]. Many studies demonstrated that plant extracts are highly effective against bacterial microorganisms due to enormous kind of secondary metabolites [33,34,35].…”

Section: Discussionmentioning

confidence: 99%

Phytochemical Screening and Anti-microbial Activity of Polygala sadebeckiana Gurke extracts on bacterial isolate from wound samples of patients with “Shimetere”

Zeleke,

Mekonnen,

Bireda

et al. 2023

Preprint

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Background As Guraghe community believed that modern medication use especially through injection route seriously aggravate the disease, the root part of Polygala sadebeckiana Gürke is commonly used as traditional medicine in the management of “Shimetere”, which is a common skin and soft tissue infection in the community. The aim of this study was to evaluate anti-microbial activity of Polygala sadebeckiana Gürke extract on bacterial isolate from wound samples of patients with “Shimetere”. Methods Agar well diffusion method was used to evaluate antibacterial activity and agar dilution method was utilized to determine minimum inhibitory concentrations (MIC) and minimum bactericidal concentrations (MIC). The crude extract was tested against isolated bacteria at the concentration of 25, 50, 75 and 100 mg/mL in triplet (3x). The positive controls were azithromycin (15µg) cloxacillin disk (5µg) and the negative control was dimethylsulfoxide (5%). The group means comparisons were made using one-way ANOVA at a significance level of p < 0.05 and the results were presented as mean ± standard deviation. The presence of secondary metabolites from crude extract was checked by standard testing procedures. Results S. aurous and S. pyrogen were the two identified bacteria from 9 (60%) and 3(20%) of wound samples, respectively. All identified bacterial strains were susceptible for the reference antibiotics. Tannins and saponins were the most abundant secondary metabolites found in the crude extracts. The average inhibition zone of the plant extract with 100, 75, 50 and 25 mg/mL concentration were 27, 20.33, 15.25, 11.96 mm (p < 0.000) for S.aurous and 30.02, 24.50, 19.07, 15.77 mm (p < 0.000) S.pyrogen bacteria. MIC and MBC of the crude extract were 1.67 and 10 mg/ml for S. aureus and 0.98 and 4 mg/ml for S.pyrogen. Conclusion Polygala sadebeckiana Gürke contained significant tannins and saponin as a secondary metabolites and had antibacterial activities against isolated bacteria (S. aurous and S. pyrogen ) from “Shimetere”.

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Phytochemical Screening, Antioxidant, and Antimicrobial Activities of Seven Underinvestigated Medicinal Plants against Microbial Pathogens

Cited by 18 publications

References 41 publications

Assessing the Efficiency of Antimicrobial Plant Extracts from Artemisia afra and Eucalyptus globulus as Coatings for Textiles

Assessing the Efficiency of Antimicrobial Plant Extracts from Artemisia afra and Eucalyptus globulus as Coatings for Textiles

Biofilm Formation and Phospholipase and Proteinase Production in Cryptococcus neoformans Clinical Isolates and Susceptibility towards Some Bioactive Natural Products

Phytochemical Screening and Anti-microbial Activity of Polygala sadebeckiana Gurke extracts on bacterial isolate from wound samples of patients with “Shimetere”

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