Phytochemical screening, antioxidant activity, total polyphenols and flavonoids content of different extracts of propolis from Tekel (Ngaoundal, Adamawa region, Cameroon)

Abstract

Five extracts of propolis of Adamawa Cameroon were obtained by percolation and maceration with five different solvents: hexane, ethyl acetate, ethanol, methanol and water, in order of increasing polarity. Phytochemical screening was carried out on the extracts and the total content in flavonoids and polyphenols were evaluated by photometric methods. The total flavonoid content was evaluated using the Neu reagent (2-aminodiethyl diphenylborinate) and quercetin as standard and the results varied from 0.84±0.02 gQE/100gRM in ethyl acetate extract to 1.52±0.06 gQE/100gRM in ethanol extract. The total polyphenol content was evaluated using Folin-Ciocalteu reagent and gallic acid as standard and results varied from 2.32±0.37 gGAE/100gRM in the ethyl acetate extract which is the least to 8.64±0.47 gGAE/100gRM in the aqueous extract. The antiradical activities of the extracts were evaluated through their inhibition on DPPH• and IC50 values varied from 1.88 mg/mL in the aqueous extract which showed highest antioxidant power to 5.06 mg/mL in the ethyl acetate extract with the least antioxidant power. BHT and vitamin C were used as synthetic and natural standards respectively and they showed higher antioxidant power compared to the propolis extracts. Ferrous iron chelating capacities of the extracts were determined using potassium ferricyanide reagent and EDTA as standard. Using Stat Graphics software and Durbin-Watson statistics test, the extracts showed significant correlation between flavonoid content and polyphenol content with DPPH• scavenging activity. The ethyl acetate extract showed least ferrous ion chelating capacity while the methanol extract showed highest ferrous ion chelating capacity.

Antibiofilm and Anti-Quorum Sensing Potential of Cycloartane-Type Triterpene Acids from Cameroonian Grassland Propolis: Phenolic Profile and Antioxidant Activity of Crude Extract

“…The use of synthetic antioxidants is on a decline because compounds such as butylated hydroxytoluene (BHT) and butylated hydroxyanisole (BHA) may have adverse effects and as a result, there is a need to identify alternative natural sources of food antioxidants [ 30 ]. Propolis extracts are consumed by people from different parts of the world in the form of alternative medicine, food complements and nutraceuticals since they have antioxidant properties and can contribute protect living tissues from cellular damage caused by oxidative agents and free radicals [ 31 ].…”

HPLC-DAD Phenolic Composition, Antioxidant, Anticholinesterase, Antidiabetic and Anti-quorum Sensing Properties of Bitter Kola (Garcinia kola) and Kolanut (Cola acuminata)

“…[56][57][58][59] The presence of reactive oxygen species and reactive nitrogen species in the human body can cause oxidative stress and related illnesses though they are useful in some physiological activity, but harmful when they are in excess. [60][61][62] Both plants can serve as natural antioxidants and food preservatives. G. cola has shown to more active and this good antioxidant activity of this plant has been reported in other studies.…”

The effects of hive materials on phytochemical and biological properties of honeybee propolis

“…From our study, the reported quantities of phytochemicals and bioactivities are in accordance with those earlier reported. 4,[15][16][17][18][19] Previous studies on total phenols have been performed in different regions of the world, for example, in Algeria 0.8-9.0 mg GAE/g, 20 Mexico 68-500 mg GAE/g, 21 Spain 200-340 mg GAE/g, 22 Turkey 34.5-259.4 mg GAE/g 23 and on total flavonoids in Turkey 21.3-152.6 mg QE/g, and Mexico 13-379 mg QE/g, Algeria 0.6-3.5. All these results are consistent with our data, except for some variation because of the influence of vegetation on the chemical properties of propolis.…”