Phytochemical screening, antimicrobial activity and cytotoxicity of Nepalese medicinal plants Swertia chirayita and Dendrobium amoenum

Shrestha, Pritish; Bista, Manisha; Sharma, Priyadarshani; Shrestha, Sujita; Lamichhane, Basanta; Adhikari, Sandeep; Pandey, Binayak Raj; Shrestha, Bhupal Govinda

doi:10.3126/njb.v3i1.14231

Cited by 17 publications

(13 citation statements)

References 5 publications

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“…This indicates that the methanol extract is more potent on the cell lines used. The antitumor and anticancer activities of crude extracts of Dendrobium were explained in previous studies (Devi et al 2009;Lam et al 2015;Shrestha et al 2015;Sun et al 2016;Paudel et al 2017). In our previous study, we quantified the total phenolic and flavonoid contents in the crude extract of D. amoenum (Paudel et al 2015).…”

Section: Discussionmentioning

confidence: 75%

Cytotoxic activity of crude extracts of Dendrobium amoenum and detection of bioactive compounds by GC-MS

Paudel

Pant

2018

Botanica Orientalis

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Dendrobium amoenum is an epiphytic orchid used as tonic because it has many derivatives of phenols. The crude extract of this orchid has been shown to have antioxidant activity. The objectives of this research are to explore the cytotoxic activity of antioxidant-rich crude extract against the human cervical carcinoma and glioblastoma cell lines by MTT assay and to detect the compounds by GC-MS. Methanol (DAM) extract of D. amoenum showed high cell growth inhibition percentage against the tested cell lines. DAM extract showed high cytotoxic activity against HeLa cells (IC50 – 110.22 μg/ml) and least activity against U-251 cells (IC50 – 550.55 μg/ml). Thirteen compounds were detected and identified in the extract. Based on abundance, four major compounds detected were: (E)-13-docosenoic acid; oleic acid; 11-octadecenoic acid, methyl ester; and hexadecanoic acid, 2,3-dihydroxypropyl ester. The cytotoxic activity of DAM extract is probably due to the presence of these bioactive compounds, confirmation of which needs further investigation. The result also highlighted the potential of this orchid as the source of natural anticancer drug and to explore their isolation, identification and characterization.Botanica Orientalis – Journal of Plant Science (2017) 11: 38–42

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Section: Discussionmentioning

confidence: 75%

Cytotoxic activity of crude extracts of Dendrobium amoenum and detection of bioactive compounds by GC-MS

Paudel

Pant

2018

Botanica Orientalis

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“…The process was repeated until the chlorophyll pigment was completely extracted in hexane. Then the final extract was collected in Eppendorf tube after the extract was dried of solvent on water bath at 50˚C [8]. Various concentrations were then made by dissolving in Dimethyl Sulfoxide (DMSO).…”

Section: Solvent Extractionmentioning

confidence: 99%

Study of Phytochemical, Antioxidant and Antimicrobial Activity of Artocarpus heterophyllus

Thapa

Bhandari

et al. 2016

Nepal J Biotechnol

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In today’s world, search for natural medicines is increasing as a result of drug resistance of pathogens and also due to negative consequences of antibiotic. Presence of phytochemicals, antioxidant potential and antimicrobial activity of Artocarpus heterophyllus was carried out in this study. Leaf of this plant was subjected to warm extraction with three different solvents namely methanol, aqueous methanol and ethyl acetate. Leaf extract showed the presence of coumarin, alkaloid, terpenoid in methanol solvent; tannin, coumarin, saponin in aqueous methanol extract and coumarin, terpenoids in ethyl acetate solvent. Further, antimicrobial activity was assessed through disc diffusion method with six pathological bacteria and two fungi strains in four different concentrations of plant extract. Largest ZOI of 16mm was obtained against B. subtilis in 200mg/ml concentration for ethyl acetate extract. Antioxidant potential was measured by DPPH (Diphenyl-2-picrylhydrazyl) assay. DPPH free radical Scavenging Activity was expressed in % inhibition with L Ascorbic acid as standard and leaf extract in methanol showed the best activity.

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“…The presence of flavonoids, and saponins in isolation or in synergy in these crude extracts used in the study might also be responsible for exhibiting anticancer efficacies. There are testaments signifying biological interactions of flavonoids, polyphenols, or phenolic compounds [35]. Figure 1, 2, 3, and 4. B. variegata has thus been proven to be an important candidate to be used as an anti-microbial, anti-oxidant, anti-inflammatory, phytotoxic and anticancer agent.…”

Section: Results Of Phytotoxicity Assaymentioning

confidence: 99%

Study of Phytochemical, Anti-Microbial, Anti-Oxidant, Phytotoxic, and Immunomodulatory Properties of Bauhinia variegata

Bhandari

Thapa²,

Niraula³

et al. 2017

JTLS

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In this paper, we investigated the anti-microbial, anti-oxidant, phytotoxic and immunomodulatory activity of plant Bauhinia variegata and also established the presence of important phytoconstituents which might signify its anticancer property. Phytochemical screening, anti-microbial assay, phytotoxicity assay, anti-oxidant assay and immunomodulatory analysis was done according to the protocol established at Department of Biotechnology, Kathmandu University and Panjwani Center for Molecular Medicine and Drug Research, University of Karachi, Pakistan. In the phytochemical screening, we observed the presence of different phytochemicals like steroids, terpenoids, flavonoids, reducing sugars and glycosides. In case of antimicrobial assay, the plant was found to be most effective against Bacillus cereus with the highest zone of inhibition (ZOI) of 12 mm and against Rhizopus with ZOI of 11 mm. During the antioxidant assay in comparison to ascorbic acid; at highest concentration, the scavenging activity shown by the plant B. variegata are 43.38% as compared to ascorbic acid 89.25%. In the immunomodulatory assay at the highest concentration i.e. 250 µg/mL, the plant showed 75.1% effect, which showed that the plant has potential anti-inflammatory potential. In phytotoxicity assay, B. variegata showed 20% phytotoxicity. B. variegata has thus been proven to be an important candidate to be used as an antimicrobial, antioxidant, anti-inflammatory, phytotoxic and anticancer agent. Separation of pure compounds with bioassay guided extraction, spectrometric analysis and subsequent cytotoxicity assay of the pure bioactive compounds from B. variegate is highly recommended as crude extract itself showed promising phytotoxicity and other pharmaceutical potential.

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Phytochemical screening, antimicrobial activity and cytotoxicity of Nepalese medicinal plants Swertia chirayita and Dendrobium amoenum

Cited by 17 publications

References 5 publications

Cytotoxic activity of crude extracts of Dendrobium amoenum and detection of bioactive compounds by GC-MS

Cytotoxic activity of crude extracts of Dendrobium amoenum and detection of bioactive compounds by GC-MS

Study of Phytochemical, Antioxidant and Antimicrobial Activity of Artocarpus heterophyllus

Study of Phytochemical, Anti-Microbial, Anti-Oxidant, Phytotoxic, and Immunomodulatory Properties of Bauhinia variegata

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