Phytochemical Screening, Antimicrobial Activity and Brine Shrimp Lethality Bioassay of Different Extracts of Alysicarpus Vaginalis Var. Nummularifolius (Dc.) Miq. (Family: Fabaceae)

Kurian, Susan; Josekumar, V. S.

doi:10.22159/ijpps.2017v9i1.15688

Cited by 13 publications

(17 citation statements)

References 12 publications

(13 reference statements)

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“…In addition, P. major calli with the highest growth rates contained the lowest levels of flavonoids. This is why for industrial production of secondary metabolites a great number of protocols involve two steps: The first one involves tissue or cell culture in a growth medium followed by a subculture step using a production medium [26,27].…”

Section: Discussionmentioning

confidence: 99%

Micropropogation and Analysis of Phytochemical Profile of Tissue Culture Grown Plantago Ovata Forsk.

Sharma

Kumari²,

Mahant³

2017

Asian J Pharm Clin Res

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Objective: Plantago ovata is an important medicinal plant of the Himalayan region which is greatly used in herbal drugs manufacturing. The plant is multipurpose constrained in Himalayan regions. Plantago has many medicinal properties such as antioxidant, anti-inflammatory, hematopoiesis effects, and used for the treatment of cancer. Plantago contains copious amount of mucilage that is able to treat diarrhea, constipation, and act as an effective weight loss aid. The plant being medicinal possesses complex phytochemicals. The investigation of various Plantago organs (leaves, stem, etc.) revealed their high potential to produce a wide array of bioactive secondary metabolites. In the present study, a new method of micropropagation through tissue culture was developed for Plantago so as to meet the future demand of plant. Further, a morphological and physiochemical comparison of tissue culture grown plant was done with in vivo grown plants.Methods: P. ovata was grown in vitro through tissue culture technique using Murashige and Skoog media and in vivo in the nursery area of Shoolini University. The tissue culture of P. ovata Forsk was managed to restrict the ecological factors. Experimentally, parameters including germination growth and phytochemical constituents of plant in vivo and in vitro conditions were observed. Results:The result revealed an easy method for the micropropagation of Plantago. However, changes in the concentration of phytochemical constituent's in tissue culture grown Plantago were reported in comparison to in vivo grown plants. Phytochemicals constituents (carbohydrate, chlorophyll, saponin, and tannin) were reduced in tissue culture grown plant whereas some phytochemicals (phenol, alkaloid, Flavonoid, protein, and phytosterol) increased in tissue culture grown plant than in vivo plant. A reduction in morphological trait was found in tissue cultured plant. Conclusion:The developed tissue culture method for the micropropagation of P. ovata can be proved as a landmark to meet the industrial need in the near future.

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Section: Discussionmentioning

confidence: 99%

Micropropogation and Analysis of Phytochemical Profile of Tissue Culture Grown Plantago Ovata Forsk.

Sharma

Kumari²,

Mahant³

2017

Asian J Pharm Clin Res

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“…The solution was filtered using Whatman no. 4 filter paper and the filtrate was concentrated to a semi-solid residue in a rotary vacuum evaporator and put away in the refrigerator for further anti-microbial analysis [5]. The extract acquired was weighed and the rate yield was figured as far as the dried weight of the plant material utilizing the equation:…”

Section: Preparation Of the Plant Extractmentioning

confidence: 99%

Preliminary Phytochemical Screening and in Vitro Anti-Microbial Activity of Ethanolic Extract of Delonix Regia (Hook) Raf. Bark (Family-Leguminosae)

Gupta¹,

Dash²,

Paul³

et al. 2018

Int J Curr Pharm Sci

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Objective: To estimate the anti-microbial activity of ethanolic extract of Delonix regia (Hook.) Raf (family-Leguminosae) in association with phytochemical analysis.Methods: The ethanolic extract of the bark of Delonix regia (Hook.) Raf was prepared and investigate for phytochemical constituents using standard methods. Antimicrobial activity of ethanolic extract of were carried out against one Gram positive bacteria= Staphylococcus aureus and one Gram negative bacteria= Escherichia coli. The anti-fungal activity of the plant extract was evaluated on Candida albicans. The testing was done by the disc diffusion method. A zone of inhibition of ethanolic extract was compared with that of standard Amikacin for anti-bacterial activities and Fluconazole for anti-fungal activity.Results: The present investigation shows the phytochemical analysis, antimicrobial activity of the ethanolic extract of the bark of Delonix regia (Hook.) Raf. Various phytochemical analyses revealed the presence of alkaloids, anthraquinone, phenol, terpenes, cardenolides, phlobatannins, tannin, chalcones, steroids. The anti-microbial activity of the ethanolic extract of the plant showed significant results against all three of the test organisms.Conclusion: The present study concluded that ethanolic extract of the bark of Delonix regia (Hook.) Raf contains the high existence of phytochemicals. The ethanolic extract of the plant was found to possess promising antimicrobial activity when compared with the standards.

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“…At first, the plant material was defatted with petroleum ether then the extraction is carried out in a hydroalcoholic solvent that is methanol and distilled water in a Soxhlet apparatus by continuous heat extraction. The extract was concentrated in a rotary flash evaporator at a temperature not exceeding 50 °C [5].…”

Section: Preparation Of the Plant Extractmentioning

confidence: 99%

Preliminary Phytochemical Screening and to Evaluate the Anthelmintic Activity of Hydro-Alcoholic and Petroleum Ether Extract of Nyctanthes Arbour-Tristis Root. (Family-Nyctaginaceae)

Abdullah¹,

Das²

2018

Int J Curr Pharm Sci

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Objective: To estimate the anthelmintic activity of hydro-alcoholic (methanol) and petroleum ether extract of Nyctanthes arbour-tristis (family-Nyctaginaceae) in conjugation with phytochemical screening.Methods: The hydro-alcoholic and petroleum ether extract of the whole root part of the plant Nyctanthes arbour-tristis (family-Nyctaginaceae) was prepared. And studies Phytochemical constitutes by the various standard method. The anthelmintic activity of the plant was performed by a different extract of plant material were tested against adult Indian earthworms pheretima posthuma as test worms, the bioassay determines the time of paralysis and time of death. Albendazole is used as a standard reference drug.Results: The present study shows the phytochemical analysis, the anthelmintic activity of the hydro-alcoholic and petroleum ether extract of the root of Nyctanthes arbour-tristis. Various phytochemical analyses revealed the presence of alkaloids, carbohydrates, flavonoids, tannin, phenol,, glycosides, saponins respectively. The anthelmintic activity of the plant extract showed significant results against all earthwormsConclusion: The study has shown that petroleum ether and methanolic extract of Nyctanthes arbortristis root have significantly determined anthelmintic activity. But the methanolic extract of Nyctanthes arbortristis root shown most significant anthelmintic activity as compared to petroleum ether extract.

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Phytochemical Screening, Antimicrobial Activity and Brine Shrimp Lethality Bioassay of Different Extracts of Alysicarpus Vaginalis Var. Nummularifolius (Dc.) Miq. (Family: Fabaceae)

Cited by 13 publications

References 12 publications

Micropropogation and Analysis of Phytochemical Profile of Tissue Culture Grown Plantago Ovata Forsk.

Micropropogation and Analysis of Phytochemical Profile of Tissue Culture Grown Plantago Ovata Forsk.

Preliminary Phytochemical Screening and in Vitro Anti-Microbial Activity of Ethanolic Extract of Delonix Regia (Hook) Raf. Bark (Family-Leguminosae)

Preliminary Phytochemical Screening and to Evaluate the Anthelmintic Activity of Hydro-Alcoholic and Petroleum Ether Extract of Nyctanthes Arbour-Tristis Root. (Family-Nyctaginaceae)

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