In our previous work we have reported wound healing activity of leaves extract of Rhynchosia rothii in which we got excellentactivity. Therefore in present study, the development of Rhynchosia rothii loaded Lycoat RS 720-BSA conjugated polymericnanoparticles and subsequent ointment formulation has been aimed. Maillard Reaction was used to develop the Lycoat RS 720-BSAconjugate. The solvent evaporation approach was used to produce nanoparticles with Rhynchosia rothii loaded on them. Thenanoparticles had a 257nm particle size and exhibited a spherical shape. The zeta potential in the formulation was -22.4 mV.Entrapment efficiency was 88.32% in the enhanced batch. The extracted content of the optimized batch was found to be 78.10%. Acontinuous release pattern was found by the in-vitro diffusion investigation, with 94.24% diffusion after 24 hours. The Rhynchosiarothii extract was created as an absorbent ointment formulation with a pH of 6.80–6.92 and a spread ability of 80.00–110.16 g.cm/s.It also has a distinctive aroma. Ointment containing herbal nanoparticles from the F4 batch has produced positive results. A newmethod of promoting nanoparticles in herbal medication delivery systems is by employing them in nanoparticles and an ointment.From present investigations we concluded that prepared ointment can be used clinically for the treatment of wounds if optimizedfurther using more in vitro and in vivo models along with toxicity predictions.