Phytochemical Screening and Hypoglycemic Property of Globimetula braunii (Loranthaceae) Leaf Extracts

Okpanachi, Gideon O.; Oyi, Avosuahi Rukayat; Musa, H.; Abdulsamad, Abdulrahman; Sani, M B; Ya’u, Jamilu

doi:10.9734/jpri/2018/39870

Cited by 3 publications

(3 citation statements)

References 4 publications

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“…The ethanol leaf extract of G. braunii had previously been reported to show hypoglycaemic effect in alloxan-induced diabetic rats at 250 and 500 mg/kg after 2 and 4 h [27]. This result further supported insulin stimulation as the main mechanism of action of G. braunii leaf extract that was suggested by the results of this present work (Table 1).…”

Section: Antihyperglycaemic Effects Of the Extractsupporting

confidence: 89%

Two antihyperglycaemic compounds from Globimetula braunii (Engl.) Van Tiegh (Loranthaceae)

Ayoola

Oluwabunmi

Olatubosun

et al. 2020

GSC Biol. and Pharm. Sci.

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Large numbers of medicinal plants are constantly being screened for possible pharmacological values, especially for chronic diseases such as diabetes, with the view of discovering new compounds that may serve as templates for synthesis of more active/less toxic drugs. Therefore, the antihyperglycaemic activity of the leaf of Globimetula braunii with antidiabetic ethnomedical usage in Nigeria was investigated is this study to justify this folkloric claim. The median lethal dose, LD50 of the ethanol leaf extract of G. braunii was determined using Lorke's method and its antihyperglycaemic effect at 100, 200 and 400 mg/kg was evaluated using glucose-induced hyperglycaemic rats while glibenclamide (5 mg/kg) and 1 % Tween 80 in normal saline served as positive and negative controls, respectively. Anti-hyperglycaemic activity-directed purification of the extract of the plant in glucose-loaded rats, led to the isolation and characterisation of phyllanthone and methyl 2, 6-dihydroxy-4-methoxybenzoate from the dichloromethane partitioned fraction. The findings showed that the LD50 of the ethanol leaf extract of G. braunii was greater than 5,000 mg/kg while its 100 mg/kg was the most active dose with comparable activity (p>0.05) to the standard drug, glibenclamide. The dichloromethane and aqueous partitioned fractions of the extract were the most promising fractions. Chromatographic separations of the dichloromethane fraction yielded phyllanthone, and methyl 2, 6dihydroxy-4-methoxybenzoate that elicited comparable activity to glibenclamide (5 mg/kg) at 10 and 20 mg/kg at all time-points. The study justified the antidiabetic folkloric use of G. braunii leaf and confirmed phyllanthone and methyl 2,6-dihydroxy-4-methoxybenzoate as two of its antihyperglycaemic constituents.

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Section: Antihyperglycaemic Effects Of the Extractsupporting

confidence: 89%

Two antihyperglycaemic compounds from Globimetula braunii (Engl.) Van Tiegh (Loranthaceae)

Ayoola

Oluwabunmi

Olatubosun

et al. 2020

GSC Biol. and Pharm. Sci.

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“…Also, the petroleum ether fraction was found to have the most significant effect. 18 Similarly, the ethanol leaf extract of G. braunii was found to have an antihyperglycemic effect comparable to that of glibenclamide. However, the dichloromethane and aqueous partitioned fractions of the ethanol leaf extract were found to be the most promising fractions with antihyperglycemic properties.…”

Section: Scientific Studies On G Brauniimentioning

confidence: 95%

Phytochemical Constituents and Pharmacological Activities of Globimetula braunii (Loranthaceae): A Review

2022

TJNPR

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Globimetula braunii (Loranthaceae) is a hemiparasitic shrub that grows on dicotyledonous trees; the plant is used traditionally in the treatment of various diseases. This study aims to review the ethnomedicinal uses, scientific and pharmacological studies as well as phytochemistry of G. braunii. Information related to the ethnomedicinal uses, scientific and pharmacological studies as well as phytochemistry of the G. braunii was obtained by searching using the keyword G. braunii in the following databases; Google Scholar, PubMed, ScienceDirect and Web of Science. The literature search revealed that G. braunii is widely distributed in tropical countries like Malaysia, Cameroun, Ghana and Nigeria, and is used traditionally to treat pulmonary diseases, hypertension, ulcer, cancer, epilepsy and pain. Scientific studies have shown that G. braunii possessed anticonvulsant, central nervous depressant, oxytocic, analgesic, antiinflammatory, laxative, antihypertensive, anti-hyperglycemic, antioxidant, anticancer, antibacterial, antifungal and antiplasmodial activities. Approximately, thirty-two (32) compounds were isolated from the plant. Some of these compounds include; 7α,15α-epoxy-lup-20(29)-ene-30-hydroxyl-3β-O-tetracosanoate, Lupeol, quercetin, (+)-catechin, quercitrin, rutin, avicularin, and 6-methoxy-2H-inden-5-ol. G. braunii should be used in the development of new plant-derived drugs and used as herbal supplements for the management of various diseases. In addition, studies on the clinical safety and efficacy of the plant should also be carried out.

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“…This claim has not been scientifically substantiated. Although some phytochemical and pharmacological studies have been carried out on G. braunii, 21 to the best of our knowledge, there is paucity of information on the antiplasmodial activity of the plant.…”

Section: Introductionmentioning

confidence: 99%

Antimalarial and Erythrocyte Membrane Stability Properties of Globimetula braunii (Engle Van Tiegh) Growing on Cocoa in Plasmodium berghei-Infected Mice

Olanlokun¹,

Ekundayo²,

Ebenezer³

et al. 2021

IDR

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Introduction Resistant malaria is a fatal disease. Globimetula braunii (African Mistletoe) is traditionally used for malarial treatment but this fact has not been scientifically reported. Methods Plasmodium berghei (NK65)-infected male Swiss mice (20±2 g) were treated orally and once daily with 100, 200, and 400 mg/kg BW of methanol extract and its respective hexane, dichloromethane and ethyl acetate fractions for 9 days. P-alaxin was used as control drug P. berghei (ANKA)-infected mice were then treated with the most potent fraction for 5 days. Parasitemia and parasite clearance were determined by microscopy, while hematological parameters, heme, hemozoin, and mouse erythrocyte membrane stabilisation were assayed. The phytochemicals in the most potent fraction were identified using gas chromatography-mass spectrometry. Results Hexane fraction (HF)-treated mice (400 mg/kg BW) had the least mean parasite load (0.00 ± 0.00; 0.14 ± 0.05%) and highest clearance (100 ± 0.00; 75.50 ± 4.95%) compared with infected control (9.81 ± 0.09; 6.84 ± 0.09%) in susceptible and resistant models, respectively. Hexane fraction modulated hematological indices, minimised erythrocyte membrane damage in heat-induced (2.18 ± 0.94%) and hypotonic solution-induced (7.93 ± 0.93%) compared to artequin (5.05 ± 2.18; 6.38 ± 0.33%) and P-alaxin (67.45 ± 5.15; 56.78 ± 1.10%) in both models of membrane stabilisation, respectively. Hexane fraction ( P <0.01) increased heme and decreased hemozoin contents. Friedelan-3-one was identified as the most abundant triterpene. Conclusion The results indicated that G. braunii has anti-plasmodial properties and minimally dis-stabilised erythrocyte membrane. The major findings in this study are that n -hexane fraction of G. braunii possess excellent and moderate antiplasmodial activity against susceptible and resistant P. berghei , respectively. This was reflected via decreased parasite load, improved hematological parameters, increased heme and decreased hemozoin contents. Friedelan-3-one, a major constituent of the n-hexane fraction, may be responsible for this activity.

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Phytochemical Screening and Hypoglycemic Property of Globimetula braunii (Loranthaceae) Leaf Extracts

Abstract: Aims:To carry out phytochemical screening, acute toxicity test and to investigate the hypoglycemic potential of the ethanol extract and fractions of Globimetula braunii leaves.

Cited by 3 publications

References 4 publications

Two antihyperglycaemic compounds from Globimetula braunii (Engl.) Van Tiegh (Loranthaceae)

Two antihyperglycaemic compounds from Globimetula braunii (Engl.) Van Tiegh (Loranthaceae)

Phytochemical Constituents and Pharmacological Activities of Globimetula braunii (Loranthaceae): A Review

Antimalarial and Erythrocyte Membrane Stability Properties of Globimetula braunii (Engle Van Tiegh) Growing on Cocoa in Plasmodium berghei-Infected Mice

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