Phytochemical Screening and Evaluation of in-Vitro Antimicrobial Activity of Drosera Spatulata Var Bakoensis- an Indigenous Carnivorous Plant Against Respiratory Tract Infectious Microbes.

Abstract

Objective: In the present study, phytochemical constituents of Drosera spatulata var. bakoensis have been evaluated, and antimicrobial activity was screened against respiratory tract infectious microbes. Methods:The phytochemicals present in D. bakoensis by qualitative phytochemical assays and aqueous, ethanol, methanol extracts of thick roots, open flower, and hair of D. bakoensis against this pathogenic bacteria and fungi showed a high zone of inhibition which estimated by disc diffusion method as well as minimum inhibitory concentration (MIC) manifestation by the broth microdilution assay followed minimum bactericidal concentration (MBC) and biofilm inhibitory concentrations (BICs) were determined against Staphylococcus aureus, Klebsiella pneumoniae, and Staphylococcus pneumoniae the causative organisms of pulmonary infections, mainly effects the nasal pharynx, trachea, and lungs as well as Aspergillus niger. Results:The values of MIC, MBC, and BIC obtained were between 0.3-0.9, 0.36-2.25, and 0.12-0.37 mg/mL, respectively. In the antibacterial and antifungal activity, results revealed that ethanol and methanol extracts significantly showed activity against the tested respiratory disease causing bacteria and antifungal properties to the zone of inhibition showed more than aqueous extracts at very low concentrations. Conclusion:The plant extracts of D. bakoensis have high potential even at low concentrations values against bacteria and fungi cultures, and these results validated by the presence of high amounts of alkaloids, quinones, anthraquinones, and flavonoids in the plant extracts which have tremendous impact against respiratory infectious microbes.