Phytochemical Screening and Characterization of Agarwood (Aquilaria malaccensis) Chips Wood Grade as Incense Headspace Volatile Compounds by GC-MS.Ms.Q.TOF, SPME

Abstract

Agarwood (Aquilaria malaccensis) is an Asian tree traditionally used for many medicinal purposes, its oil constituents and inhaled vapor has sedative, antidepressant and anxiolytic activities. Gas Chromatography and quadruple time of flight –mass spectrometry (GC.MS.MS/Q.TOF) in the MS-MS mode has been used in screening and identification of multiple compounds of three different grade of agarwood chipwood, using DB-1 column which led to identification of 96 compounds while the majority of the compounds about 500 compounds remain UN knowing to the difficulties of confirming or rejecting them . The generated results were so complicated and very difficult to be interpreted for the presence of isomers with similar matched spectrums; the absence of official standard references for these compounds make the judgment of confirming the identified compounds more complicated and very hard to absolute identification to be assessed. The compounds were extracted by applying a solvent less technique; trapping incense and volatile headspace. The total Ion chromatogram detected compounds are distributed among different array over the chemical families among monoterpenes, sesquiterpenes, hydrocarbons, oxygenated monoterpenes, sesquiterpenes, norterpenoids and diterpenoids ,short chain glycols, carboxylic acids and others. The flowing compounds are the major terpene, sesquiterpene and oxygenated sesquiterpenoid found in all grades of A. malaccensis chipswood under investigation, furthermore; the study represented that the characterization of compounds from agarwood chips wood is highly possible through incense volatile sample.