Phytochemical Screening and Antioxidant Activities of the Seedling Extracts from Inca Peanut Plukenetia volubilis

Abstract

Background: Plukenetia volubilis L., Inca peanut is an oleaginous plant, widely cultivated as commercially in South East Asia, especially in Thailand. The oil from the seed plant is a greatest interesting a natural source. Objectives: The aims of this study were investigated phytochemical screening, to evaluated the total flavonoids and phenolic compound contents as well as antioxidant activities of seedling extract from P. volubilis. Methods: The dried P. volubilis seedlings of 21 days growing period were extracted by using different solvent including aqueous (ASS), 50% ethanolic (HESS), and 95% ethanolic (ESS) extracts. The phytochemical screenings were determined on total phenolic compound (TPC) and flavonoid (TFC) contents. The antioxidation were tested by using 2,2-diphenyl-1-picrylhydrazy radical scavenging (DPPH), 2,2-azinobis-(3-ethylbenzothiazoline-6-sulphonate) (ABTS +) assay, and ferric reducing antioxidant power (FRAP). Results: The results found that the ESS were significantly highest amount on total phenolic compound (23.0809±0.8632 mgGE/gExt) and flavonoid (466.3839±1.5580 mgQE/gExt) contents. In this study, ascorbic acid (IC 50 =0.016±0.0003 mg/mL) and Trolox  (IC 50 =0.044±0.0008 mg/mL) as standard substances were showed more potent than all of the extracts from P. volubilis seedlings. Surprisingly, the ESS has more potent on free radical scavenging higher than different solvents; DPPH=0.007± 0.001 (IC 50 =mg/mL), ABTS= 1.4065± 0.0505 (IC 50 =mg/mL),and FRAP= 74.4960± 2.6067 (mg=TE/gExt). Conclusion: the plant seedling extracts composed with high amount of flavonoids and phenolic compound contents possess valuable to antioxidant activities. The seedling extracts from the plant could apply to supplementary food, cosmetic, pharmaceutical, and horticultural industries. Next study, chemical compositions, the major active compound(s), and biological activities will be clarified.

In vitro bioactivities and preparation of nanoemulsion from coconut oil loaded Curcuma aromatica extracts for cosmeceutical delivery systems

“…In some plants, hydro-alcoholic and alcoholic extracts were determined total phenolic content using Folin-Ciocalteu’s reagent with gallic acid and total flavonoid content using aluminium chloride with quercetin. It was found that flavonoid content was higher than total phenolic content ( Agbo et al, 2015 , CI and Indira, 2016 , Puangpronpitag et al, 2021 ). The other active compounds of phenolic and flavonoid compounds may be used and obtained the appropriated the amount of active compounds in their extracts ( Vongsak et al, 2013 ).…”

Effect of maltodextrin concentration and drying temperature on the physicochemical characteristics of Sacha inchi (Plukenetia volubilis) extract powder

“…The results of antioxidant activity analysis on sample m 1 t 1 (5% maltodextrin, temperature 50⁰C) showed a result of 0.075%, which is the antioxidant activity of this powdered Sacha inchi extract including a weak level category. According to Puangpronpitag et al (2021), the IC 50 result on Sacha inchi seed oil is 0.007 ± 0.001 (mg/mL). Still, in this study at 50⁰C, the antioxidant activity is already weak, another factor that causes antioxidant levels in this powdered Sacha inchi extract may be due to the length of drying carried out.…”

“…Extracts of various medicinal plants are used in different industries such as pharmaceuticals, food industries and for nanomedicine (Hayat et al, 2023;Hussain et al, 2023;Iram et al, 2023;Riasat et al, 2023;Saleem et al, 2023;Sana et al, 2022;Syed et al, 2023;Zahra et al, 2023;Zawar et al, 2023;Rauf et al, 2023; Effect of maltodextrin concentration and drying temperature on the physicochemical characteristics of Sacha inchi Naveed et al, 2022a;Naveed et al, 202b). Sacha inchi (Plukenetia volubilis L.) is a shrub that grows vines and is commonly known as Sacha beans, mountain beans, or Inka beans (Puangpronpitag et al, 2021). Sacha inchi is an annual plant with slightly hairy textured leaves and belongs to the Euphorbiaceae family.…”

“…Sacha inchi is an annual plant with slightly hairy textured leaves and belongs to the Euphorbiaceae family. The plant is widely cultivated commercially in South Asia and Southeast Asia, especially in Thailand (Puangpronpitag et al, 2021). Sacha inchi in Indonesia has developed a lot, such as in the Central Java and Majalengka regions, West Java.…”

Physicochemical Characteristics, Fatty Acid Profile, and In Vitro Antioxidant Activity Evaluation of Sacha Inchi Seed Oil from Indonesia

“…The effectiveness of these mechanisms is due to the presence of chemical components such as In Peru, sacha inchi seed oil is conventionally applied as a regular skin care oil to promote skin health [5]. According to the research of Puangpronpitag et al [6], sacha inchi seed oil has significant antioxidant activity, with an IC50 value of 0.007 ± 0.001 (mg/mL). Hadzich et al [7] and Chirinos et al [8] also reported that the bioactive compounds contained in sacha inchi seed oil have the potential to act as antioxidants, including vitamin E, vitamin A, tannins, phytosterols, phenolic compounds, and terpenoids.…”