Phytochemical Screening and Antimicrobial Activity of Thuja Occidentalis Seeds Extracts Against the Isolated Common Skin Infecting Microorganisms

Abstract

Objective: This study represents the first attempt to investigate the antimicrobial activity of Thuja occidentalis seeds extracts against the isolated skin infecting microorganisms. Methods:T. occidentalis seeds were powdered and extracted continuously by Soxhlet apparatus using 96% ethanol to obtain the total crude ethanolic extract. The extracts with increasing polarity were successively prepared with petroleum ether, chloroform, ethyl acetate, and methanol using the Soxhlet apparatus.The extracts were screened for their phytochemical constituents. Their antimicrobial activity against standards microorganism and the isolated skin infecting microorganisms was evaluated using the agar diffusion method. Results:The result revealed the presence of flavonoids, alkaloids, terpenoids, tannins and cardiac glycosides. The antimicrobial activity result showed that Escherichia coli, Pseudomonas aeroginosa, Staphylococcus aureus, Bacillus subtilis and Candida albicans were found to be sensitive to the extracts of T. occidentalis seeds with inhibition zones range between 20±0.57-12±1.25 mm. The isolated skin infecting microorganisms; Staphylococcus aureus and Staphylococcus epidermidis were found to be highly susceptible to the extracts of T. occidentalis seeds with inhibition zones of 28±1.02-22±0.45 mm and minimum inhibitory concentration (MIC) values of 6.25 mg/ml. Conclusion:From the results it concludes, T. occidentalis seeds extracts had a considerable level of antimicrobial activity. They are more effective towards gram-positive than gram-negative bacteria. Hence, the active principles present in the extracts are particularly powerful effective against the skin infected isolated bacteria. In conclusion, there is a proportional increasing in the antimicrobial activity with increase of the solvents polarity. This may attribute to the fact that the chemical constituents responsible for the antimicrobial activity are of high polarity, such as tannins.

Antibacterial resistance modulatory properties of selected medicinal plants from Ghana

“…Methanol leaf extract of T. occidentalis had antibacterial activity with MIC of 4 to 10 mg/mL which is in agreement with the findings of Eltayeb and Hamid (2017). However, Sah et al (2017) reported better antibacterial activity for T. occidentalis extract which may be due to the solvent system (a mixture of ethylacetate chloroform and ethanol in a ratio of 30:30:40) used in their extraction protocol.…”

Antimicrobial Properties of Methanol Extract of Dacryodes edulis Seed and Determination of Phytochemical Composition Using FTIR and GCMS

“…The use of plant materials as medicine has been part of traditional health care in most parts of the world for thousands of years and there is still keen interest in the numerous secondary metabolites with their biological activity serving as a source of therapy in the treatment of various diseases [1]. Even though the probable mechanisms by which these secondary metabolites functions are still quite unknown, there is still widespread use of the plants [1,2]. The wide-spread use of plant-based medicinal drugs may be related to their low cost and easy accessibility as well as low incidence in adverse effects compared to synthetic drugs [2].…”

Antibacterial Activity of Moderately Volatile Components of the Oil Extracted From the Seeds of Dacryodes Edulis G. Lam

“…About 80% of the entire world population rely on alternative/ traditional medicine to provide their basic health-care needs [1]. Numerous secondary metabolites with biological activity are currently being exploited in the developing countries in medicine and alternative medicine because of lack of or inadequate health-care facilities for the treatment of ailments.…”