Phytochemical Repurposing of Natural Molecule: Sabinene for Identification of Novel Therapeutic Benefits UsingIn SilicoandIn VitroApproaches

Sharma, Shamini; Gupta, Jeena; Prabhakar, Pranav Kumar; Gupta, Pawan; Solanki, Preeti; Rajput, Anuradha

doi:10.1089/adt.2019.939

Cited by 22 publications

(10 citation statements)

References 38 publications

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“…Our PLL-EO was high in α-pinene and sabinene content when comparing with PLL-EO collected in Turkey [45] or even in other regions of Sardinia [35]. Both α-pinene and sabinene were described to exert strong antibacterial activity [46,47], and additionally, α-pinene protects cells against oxidative stress [48]. Comparing the chemical profile of the raw EO with that was reported in a previous analysis regarding PLL leaves-EO from Sardinian biodiversity [33], it can be noted that, over time, and regardless of harvesting and seasons, the concentration of such terpenoids has never been lower than 0.05%.…”

Section: Discussionmentioning

confidence: 60%

The Pharmaceutical Ability of Pistacia lentiscus L. Leaves Essential Oil Against Periodontal Bacteria and Candida sp. and Its Anti-Inflammatory Potential

et al. 2020

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Background: Given the increasing request for natural pharmacological molecules, this study assessed the antimicrobial capacity of Pistacia lentiscus L. essential oil (PLL-EO) obtained from the leaves of wild plants growing in North Sardinia (Italy) toward a wide range of periodontal bacteria and Candida, including laboratory and clinical isolates sp., together with its anti-inflammatory activity and safety. Methods: PLL-EO was screened by gas chromatography/mass spectrometry. The minimal inhibitory concentration (MIC) was determined. The anti-inflammatory activity was measured by cyclooxygenase (COX-1/2) and lipoxygenase (LOX) inhibition, while the antioxidant capacity was determined electro-chemically and by the MTT assay. The WST-1 assay was used to ascertain cytotoxicity toward four lines of oral cells. Results: According to the concentrations of terpens, PLL-EO is a pharmacologically-active phytocomplex. MICs against periodontal bacteria ranged between 3.13 and 12.5 µg/ml, while against Candida sp. they were between 6.25 and 12.5 µg/mL. Oxidation by COX-1/2 and LOX was inhibited by 80% and 20% µg/mL of the oil, respectively. Antioxidant activity seemed negligible, and no cytotoxicity arose. Conclusions: PLL-EO exhibits a broad-spectrum activity against periodontal bacteria and Candida, with an interesting dual inhibitory capacity toward COX-2 and LOX inflammatory enzymes, and without side effects against oral cells.

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Section: Discussionmentioning

confidence: 60%

The Pharmaceutical Ability of Pistacia lentiscus L. Leaves Essential Oil Against Periodontal Bacteria and Candida sp. and Its Anti-Inflammatory Potential

et al. 2020

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show abstract

“…Our PLL-EO was high in α-pinene, and sabinene content when comparing with PLL-EO collected in Turkey [45] or even other regions of Sardinia [35]. Both, α-pinene and sabinene were described to exert strong antibacterial activity [46][47] and additionally, α-pinene protects cells against oxidative stress [48]. Comparing the chemical profile of the raw EO with that reported in previous analysis regarding PLL leaves-EO from Sardinian biodiversity [33], it can be noted that over time, and regardless of harvesting and seasons, the concentration of such terpenoids has never been lower than 0.05%.…”

Section: Discussionmentioning

confidence: 58%

The Pharmaceutical Ability of Pistacia lentiscus L. Leaves Essential Oil against Periodontal Bacteria and Candida sp. and Its Anti-inflammatory Potential

Milia¹,

Usaï²,

Szotáková³

et al. 2020

Preprint

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Background: Given the increasing request for natural pharmacological molecules, this study assessed the antimicrobial capacity of Pistacia lentiscus L. essential oil (PLL-EO) obtained from the leaves of wild plants growing in North Sardinia (Italy), toward a wide range of periodontal bacteria and Candida including laboratory and clinical isolates sp., together with its anti-inflammatory activity and safety; Methods: PLL-EO was screened by gas chromatography / mass spectrometry. The minimal inhibitory concentration (MIC) was determined. The anti-inflammatory activity was measured by cyclooxygenase (COX 1/2) and lipoxygenase (LOX) inhibition while the antioxidant capacity was determined electro-chemically and by the MTT assay. The WST-1 assay was used to ascertain cytotoxicity toward four line of oral cells; Results: According to the concentrations of terpens, PLL-EO is a pharmacologically active phytocomplex. MICs against periodontal bacteria ranged between 3.13 and 12.5 µg/ml, while against Candida sp. were between 6.25 and 12.5 µg/ml. Oxidation by COX 1/2 and LOX was inhibited by 80% and 20% µg/mL of the oil respectively. Antioxidant activity seemed negligible, and no cytotoxicity arose; Conclusions: PLL-EO exhibits a broad-spectrum activity against periodontal bacteria and Candida, with an interesting dual inhibitory capacity toward COX2 and LOX inflammatory enzymes and without side effects against oral cells.

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“…The major component of the cassumunar ginger oil consists of monocyclic monoterpenoid with sabinene and terpinen-4-ol were well documented. Moreover, both sabinene and terpinen-4-ol exhibit a broad spectrum of biological effects [19,20]. Therefore, many efforts are development of cassumunar ginger oil as a value-added material that is useful as a pharmaceutical excipient for pharmaceutical, cosmetic and food industries [1,21].…”

Section: Discussionmentioning

confidence: 99%

Phytochemical and Safety Evaluations of Volatile Terpenoids from Zingiber cassumunar Roxb. on Mature Carp Peripheral Blood Mononuclear Cells and Embryonic Zebrafish

et al. 2020

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Pharmaceutical products of essential oil from Zingiber cassumunar Roxb. are extensively being developed, while the research on their safety is seldom documented. The aim of the present study was to evaluate the phytochemical profile and the effect of cassumunar ginger oil on cell-based assay and the zebrafish model. The essential oil was isolated from fresh rhizomes of Z. cassumunar using simultaneous steam-distillation. Chemical composition was analyzed using gas chromatograph coupled to a mass spectrometer (GC-MS). Effect of cassumunar ginger oil on adult carp fish peripheral blood mononuclear cells (PBMCs) was investigated using MTT assay. The embryotoxic and teratogenic effects of cassumunar ginger oil were studied in zebrafish embryos. GC-MS results showed that the essential oil was composed of sabinene (43.54%) and terpinen-4-ol (29.52%) as the major phytoconstituents. No fish PBMC cytotoxic effect was observed with the concentration less than 50 µg/mL of cassumunar ginger oil. Our results showed for the first time the embryotoxic and teratogenic effects of cassumunar ginger oil in zebrafish embryos. The result indicated that the cassumunar ginger oil induced zebrafish embryotoxicity in a concentration-dependent manner. At 500 µg/mL of cassumunar ginger oil demonstrated significantly moderated embryotoxicity within 24 h (p < 0.05). The survival rate of 100 µg/mL of cassumunar ginger group was markedly declined to zero at 96-h post-fertilization (log-rank test, p = 0.001). However, survival rates of zebrafish embryo in the 1 and 10 µg/mL cassumunar ginger groups were more than 90% throughout the trial period. Moreover, very low teratogenicity to the zebrafish embryo was also observed in 1 and 10 µg/mL of cassumunar ginger groups. Our findings suggest that there is hardly any cytotoxicity, embryotoxicity and teratogenicity at concentrations less than 10 µg/mL of cassumunar ginger oil. However, the toxicity assessment of its pharmaceutical product should prove for further consumer protection.

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Phytochemical Repurposing of Natural Molecule: Sabinene for Identification of Novel Therapeutic Benefits UsingIn SilicoandIn VitroApproaches

Cited by 22 publications

References 38 publications

The Pharmaceutical Ability of Pistacia lentiscus L. Leaves Essential Oil Against Periodontal Bacteria and Candida sp. and Its Anti-Inflammatory Potential

The Pharmaceutical Ability of Pistacia lentiscus L. Leaves Essential Oil Against Periodontal Bacteria and Candida sp. and Its Anti-Inflammatory Potential

The Pharmaceutical Ability of <em>Pistacia lentiscus</em> L. Leaves Essential Oil against Periodontal Bacteria and Candida sp. and Its Anti-inflammatory Potential

Phytochemical and Safety Evaluations of Volatile Terpenoids from Zingiber cassumunar Roxb. on Mature Carp Peripheral Blood Mononuclear Cells and Embryonic Zebrafish

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