Phytochemical Profiling of Medically Significant Crude Extract Using Gc-MS Analysis

Rajadurai, Maruthamuthu; Maithili, V.; Arunazhagi, R.; Yogesh, V.

doi:10.22159/ijcpr.2018v10i6.30966

Cited by 4 publications

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References 4 publications

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“…Sulfurous acid, decyl hexyl ester was identified in rambutan seed fat [ 44 ] and as a volatile organic compound in olive tree [ 45 ]. Tetradecane, 1-chloro- was identified in the crude extract of medicinal herbs used to treat kidney stones [ 46 ]. Dodecane, 4,6-dimethyl-, contained in the essential oil of Ephedra pachyclada , possessed antioxidant and antimicrobial activity [ 47 ].…”

Section: Resultsmentioning

confidence: 99%

Breynia cernua: Chemical Profiling of Volatile Compounds in the Stem Extract and Its Antioxidant, Antibacterial, Antiplasmodial and Anticancer Activity In Vitro and In Silico

et al. 2023

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Breynia cernua has been used as an alternative medicine for wounds, smallpox, cervical cancer, and breast cancer. This plant is a potential source of new plant-derived drugs to cure numerous diseases for its multiple therapeutic functions. An in vitro study revealed that the methanol extract of B. cernua (stem) exhibits antioxidant activity according to DPPH and SOD methods, with IC50 values of 33 and 8.13 ppm, respectively. The extract also exerts antibacterial activity against Staphylococcus aureus with minimum bactericidal concentration of 1875 ppm. Further analysis revealed that the extract with a concentration of 1–2 ppm protects erythrocytes from the ring formation stage of Plasmodium falciparum, while the extract with a concentration of 1600 ppm induced apoptosis in the MCF-7 breast cancer cell line. GC–MS analysis showed 45 bioactive compounds consisting of cyclic, alkyl halide, organosulfur, and organoarsenic compounds. Virtual screening via a blind docking approach was conducted to analyze the binding affinity of each metabolite against various target proteins. The results unveiled that two compounds, namely, N-[β-hydroxy-β-[4-[1-adamantyl-6,8-dichloro]quinolyl]ethyl]piperidine and 1,3-phenylene, bis(3-phenylpropenoate), demonstrated the best binding score toward four tested proteins with a binding affinity varying from −8.3 to −10.8 kcal/mol. Site-specific docking analysis showed that the two compounds showed similar binding energy with native ligands. This finding indicated that the two phenolic compounds could be novel antioxidant, antibacterial, antiplasmodial, and anticancer drugs. A thorough analysis by monitoring drug likeness and pharmacokinetics revealed that almost all the identified compounds can be considered as drugs, and they have good solubility, oral bioavailability, and synthetic accessibility. Altogether, the in vitro and in silico analysis suggested that the extract of B. cernua (stem) contains various compounds that might be correlated with its bioactivities.

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Section: Resultsmentioning

confidence: 99%

Breynia cernua: Chemical Profiling of Volatile Compounds in the Stem Extract and Its Antioxidant, Antibacterial, Antiplasmodial and Anticancer Activity In Vitro and In Silico

et al. 2023

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Phytochemical compounds of Halimeda opuntia from coastal waters of North Sulawesi, Indonesia

Mantiri¹,

Kepel²,

Lumingas³

et al. 2023

The 2ND INTERNATIONAL CONFERENCE ON NATURAL SCIENCES, MATHEMATICS, APPLICATIONS, RESEARCH, AND TECHNOLOGY (ICON-SMART 2021): Ma

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Bio Characterization via FTIR and GCMS Analysis of Cucurbita variety (Yellow and White Pumpkin)

Gowtham¹,

Saruniyadevi

Thenmozhi

et al. 2022

J Exp Bio & Ag Sci

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The current study aimed to conduct phytochemical screening, FTIR, and GCMS analysis in squash (Cucurbita pepo L.,) also known as a yellow and white selected pumpkin. It’s one of the dicotyledonous vegetables consumed in daily diets that imparts high inhibitor properties of inflammation, cancer, and diabetes. Traditionally it is used as an anti-helminthic remedy. The phytochemical characterization can facilitate seeking out the substance with a therapeutic property. The peel, flesh, and seed sample of each pumpkin variety were used as sources and extracted consecutively with ethyl acetate and acetonitrile using the maceration method. Phytochemical screening and quantification were carried out by standard analytical methods. The functional groups of the sample extracts were analyzed using FT-IR methods. Further, phytochemical profiling was carried out utilizing the GCMS technique to identify the therapeutically important chemicals contained in the sample. Phytochemical analysis of ethyl acetate and acetonitrile extracts showed the presence of major components like alkaloids, phenol, carbohydrate, and proteins. The farthest alkaloid, phenol, carbohydrate, and protein varied consequently for different parts like peel, flesh, and seed. The FT-IR analysis of each extract in the peel, flesh, and seed revealed that the ethyl acetate extract had the most functional groups. The major peak was characterized at wavelength 3004.24 to 3421.05 nm which indicates O-H functional group. Further quantification and GC-MS analysis were performed in ethyl acetate extract. Remarkably, GC-MS analysis of yellow and white pumpkin ethyl acetate extracts showed the utmost 6 - 8 compounds within the flesh part. Further, employing these compounds for anti-inflammatory and anti-microbial assays may aid in the discovery of new drugs for therapeutic applications.

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Phytochemical Profiling of Medically Significant Crude Extract Using Gc-MS Analysis

Cited by 4 publications

References 4 publications

Breynia cernua: Chemical Profiling of Volatile Compounds in the Stem Extract and Its Antioxidant, Antibacterial, Antiplasmodial and Anticancer Activity In Vitro and In Silico

Breynia cernua: Chemical Profiling of Volatile Compounds in the Stem Extract and Its Antioxidant, Antibacterial, Antiplasmodial and Anticancer Activity In Vitro and In Silico

Phytochemical compounds of Halimeda opuntia from coastal waters of North Sulawesi, Indonesia

Bio Characterization via FTIR and GCMS Analysis of Cucurbita variety (Yellow and White Pumpkin)

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