Phytochemical profiling, antidiabetic, antitumoral and cytotoxic potential of Psidium cattleianum Afzel. ex Sabine leaves of red variety

Abstract

In this study, phytochemical profiling, and antidiabetic, antitumoral and cytotoxic potential of aqueous extracts of leaves of red variety of Psidium cattleianum Afzel.ex Sabine were investigated. The extracts were obtained using a cellulase complex.The total phenolic compounds (TPC) were determined, and the individual phenolic compounds were quantified by HPLC-ESI-MS/MS. For the TPC, the amounts varied from 85.91 to 106.33 mg EAG g -1 . Eighteen compounds have been identified. The compounds with the highest concentrations were gallic acid, quercetin and protocatechuic acid. Antidiabetic activity was obtained through αamylase and α-glucosidase inhibition tests. The extract inhibited 17.94% of αamylase activity and 73.34% of α-glucosidase activity. The antitumoral activity in cells of cutaneous melanoma (SK-MEL-28) and the cytotoxic activity was determined in peripheral blood mononuclear cells (PBMC). The cellular migration was determined for cells SK-MEL-28. Antitumoral effects on cells SK-MEL-28 were observed and the absence of cytotoxicity on the PBMCs.

Phenolic profiling and bioactivity assessment of in vitro propagated Psidium cattleianum Sabine: A promising study

“…Meanwhile, delphinidin-3- O -galactoside is the only identified anthocyanin (9.36–2.62 μg/g). Moreover, the documentation of ellagic acid, gallic acid, isoquercitrin, quercetin, hyperoside, catechin, vanillic acid, myricetin, coumaric acid, caffeic acid, chlorogenic, ferulic, and syringic acid in the OP was expected and matched with the prior studies published about the P. cattleianum collected from Brazil, Korea, and Mexico [ [13] , [14] , [15] , [16] , [17] , [18] ]. Although there is a variation in the quantified percentages, and in the presence of unprecedented metabolites as neo-chlorogenic, delphinidine-3- O -galactoside, rutin, kaempferol-3- O -glucoside, and isorhamnetin in P. cattleianum (OP) from Egypt.…”

“…The HPLC fingerprint using natural authentic standards is a comprehensive method approved by the FDA for the assessment of the quality and reliability of plant extracts [ 12 ]. In this context, the implementation of HPLC/MS has been previously reported to investigate the phenolic phytoconstituents from P. cattleianum leaves collected from Brazil, Korea, and Mexico [ [13] , [14] , [15] , [16] , [17] , [18] ]. In addition to the prior analysis of P .…”

Confronting the complexities of antimicrobial management for Staphylococcus aureus causing bovine mastitis: an innovative paradigm

“…Besides having antimicrobial activity, phytochemicals are known to have effects on tumors, inflammation and can scavenge free radicals. have antitumor, anti-inflammatory, and antioxidant effects [ 68 ].Therefore, different phytochemical compounds offer a promising avenue for alternative therapy in combating Staphylococcus aureus -induced mastitis due to their multifaceted mechanisms of action and minimal side effects due to their ability to exert diverse pharmacological activities (Table 2 ). For instance, polyphenols like flavonoids and tannins exhibit potent antimicrobial effects by disrupting bacterial cell membranes and interfering with essential enzymatic processes.…”