Phytochemical Profiling and Evaluation of Pharmacological Activities of Hypericum scabrum L.

Jiang, Lan; Numonov, Sodik; Бобакулов, Х. М.; Qureshi, Muhammad Nasimullah; Zhao, Haiqing; Aisa, Haji Akber

doi:10.3390/molecules200611257

Cited by 50 publications

(26 citation statements)

References 13 publications

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“…All of the parts of this plant are sources of fatty acids, especially essential fatty acids, as well as effective natural antioxidants [69]. It has been indicated that, its extract possess flavonoids, such as quercetin [70]; flavonoids act as antioxidants [71]. In confirmation of our results, administration of H. scabrum Ext in rats was shown to improve learning and memory and possess antioxidant activity [27].…”

Section: Discussionsupporting

confidence: 83%

Effects of Hypericum scabrum extract on dentate gyrus synaptic plasticity in high fat diet-fed rats

et al. 2020

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High-fat diet (HFD) can induce deficits in neural function, oxidative stress, and decrease hippocampal neurogenesis. Hypericum (H.) scabrum extract (Ext) contains compounds that could treat neurological disorders. This study aimed to examine the neuroprotective impacts of the H. scabrum Ext on hippocampal synaptic plasticity in rats that were fed HFD. Fifty-four male Wistar rats (220 ± 10 g) were randomly arranged in six groups: (1) HFD group; (2) HFD + Ext300 group; (3) HFD + Ext100 group; (4) Control group; (5) Ext 300 mg/kg group; (6) Ext 100 mg/kg group. These protocols were administrated for 3 months. After this stage, a stimulating electrode was implanted in the perforant pathway (PP), and a bipolar recording electrode was embedded into the dentate gyrus (DG). Long-term potentiation (LTP) was provoked by high-frequency stimulation (HFS) of the PP. Field excitatory postsynaptic potentials (EPSP) and population spikes (PS) were recorded at 5, 30, and 60 min after HFS. The HFD group exhibited a large and significant decrease in their PS amplitude and EPSP slope as compared to the control and extract groups. In reverse, H. scabrum administration in the HFD + Ext rats reversed the effect of HFD on the PS amplitude and EPSP slope. The results of the study support that H. scabrum Ext can inhibit diminished synaptic plasticity caused by the HFD. These effects are probably due to the extreme antioxidant impacts of the Ext and its capability to scavenge free radicals.

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Section: Discussionsupporting

confidence: 83%

Effects of Hypericum scabrum extract on dentate gyrus synaptic plasticity in high fat diet-fed rats

et al. 2020

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“…The structures of all isolated compounds were elucidated by analyzing their spectral data (MS, 1 H- and 13 C-NMR, including HMQC, HMBC and DEPT) and by comparison with published literature data. They were thus identified as 3,3′,4,4′-tetra- O -methylellagic acid [ 10 ], 3,3′-di- O -methylellagic acid [ 11 ], caffeic acid [ 12 ], quercetin [ 13 ], (+)-catechin [ 14 ], (–)-epicatechin [ 13 ], (–)-epigallocatechin [ 15 ], gallic acid [ 16 ], β-sitosterol-3- O -β- d -glucopyranoside [ 17 ] and corilagin [ 18 ] ( Figure 2 ).…”

Section: Resultsmentioning

confidence: 99%

“…The enzyme activity was measured using the PTP-1B (3-(3,5-dibromo-4-hydroxybenzoyl)-2-ethylbenzofuran-6-sulfonic acid-(4-(thiazol-2-ylsulfamyl)-phenyl)-amide) enzyme inhibition assay according to the literature [ 13 , 28 , 29 , 30 ].…”

Section: Methodsmentioning

confidence: 99%

Evaluation of the Antidiabetic Activity and Chemical Composition of Geranium collinum Root Extracts—Computational and Experimental Investigations

et al. 2017

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The root of Geranium collinum Steph is known in Tajik traditional medicine for its hepatoprotective, antioxidant, and anti-inflammatory therapeutic effects. The present study was conducted to evaluate of potential antidiabetic, antioxidant activities, total polyphenolic and flavonoid content from the different extracts (aqueous, aqueous-ethanolic) and individual compounds isolated of the root parts of G. collinum. The 50% aqueous-ethanolic extract possesses potent antidiabetic activity, with IC50 values of 0.10 μg/mL and 0.09 μg/mL for the enzymes protein-tyrosine phosphatase (1B PTP-1B) and α-glucosidase, respectively. Phytochemical investigations of the 50% aqueous-ethanolic extract of G. collinum, led to the isolation of ten pure compounds identified as 3,3′,4,4′-tetra-O-methylellagic acid (1), 3,3′-di-O-methylellagic acid (2), quercetin (3), caffeic acid (4), (+)-catechin (5), (–)-epicatechin (6), (–)-epigallocatechin (7), gallic acid (8), β-sitosterol-3-O-β-d-glucopyranoside (9), and corilagin (10). Their structures were determined based on 1D and 2D NMR and mass spectrometric analyses. Three isolated compounds exhibited strong inhibitory activity against PTP-1B, with IC50 values below 0.9 μg/mL, more effective than the positive control (1.46 μg/mL). Molecular docking analysis suggests polyphenolic compounds such as corilagin, catechin and caffeic acid inhibit PTP-1B and β-sitosterol-3-O-β-d-gluco-pyranoside inhibits α-glucosidase. The experimental results suggest that the biological activity of G. collinum is related to its polyphenol contents. The results are also in agreement with computational investigations. Furthermore, the potent antidiabetic activity of the 50% aqueous-ethanolic extract from G. collinum shows promise for its future application in medicine. To the best of our knowledge, we hereby report, for the first time, the antidiabetic activity of G. collinum.

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“…Different phenolics have been reported in Hypericum species, including catechin, quercetin derivatives, phenolic acids, etc. [ 6 , 7 ], each of these compounds presenting specific chemical properties. In this work, we aimed to study the phytochemical profile and bioactivity of H .…”

Section: Introductionmentioning

confidence: 99%

Integration of in vitro and in silico perspectives to explain chemical characterization, biological potential and anticancer effects of Hypericum salsugineum: A pharmacologically active source for functional drug formulations

et al. 2018

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The genus Hypericum is one of the most popular genera in both traditional medicine and scientific platform. This study is designed to provide conceptual insights on the biological potential and chemical characterization of H. salsugineum, which is endemic to Turkey. The qualitative and quantitative phenolic content of the extracts was characterized by HPLC-ESI-MSn. Biological efficiency was investigated by enzyme inhibitory assays (cholinesterases, tyrosinase, amylase, and glucosidase) and anti-cancer efficacy tests (anti-proliferative activities with the iCELLigence technology, colony formation and wound healing scratch assays). Phenolic acids (3-O-caffeoylquinic, 5-O-caffeoylquinic, and 4-O-caffeoylquinic acids) were the predominant group in the studied extracts, although several flavonoids were also detected and quantified. The extracts exhibited good inhibitory effects on tyrosinase and glucosidase, while they had weak ability against cholinesterases and amylase. Computational studies were also performed to explain the interactions between the major phenolics and these enzymes. The extracts displayed significant anti-cancer effects on breast carcinoma cell lines. Our findings suggest that Hypericum salsugineum could be valued as a potential source of biologically-active compounds for designing novel products.

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Phytochemical Profiling and Evaluation of Pharmacological Activities of Hypericum scabrum L.

Cited by 50 publications

References 13 publications

Effects of Hypericum scabrum extract on dentate gyrus synaptic plasticity in high fat diet-fed rats

Effects of Hypericum scabrum extract on dentate gyrus synaptic plasticity in high fat diet-fed rats

Evaluation of the Antidiabetic Activity and Chemical Composition of Geranium collinum Root Extracts—Computational and Experimental Investigations

Integration of in vitro and in silico perspectives to explain chemical characterization, biological potential and anticancer effects of Hypericum salsugineum: A pharmacologically active source for functional drug formulations

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