Phytochemical Profile, Safety Assessment and Wound Healing Activity of Artemisia absinthium L.

Abstract

The aim of study was to validate, by in vitro and in vivo studies, the traditional use for wound-healing activity of Artemisia absinthium L. Reversed-phase liquid chromatography coupled with diode array detection and electrospray ion trap mass spectrometry (RP-LC-DAD-ESI-MS) analysis allowed to identify eleven polyphenols with chlorogenic acid as the most abundant compound (3.75 g/100 g of dry extract). After that, antibacterial activity as well as acute dermal and oral toxicity were assessed in animal models. In order to investigate the wound-healing activity of A. absinthium methanol extract, two ointments were formulated (MEO 5% and 10%). The ointment with the highest concentration of plant extract (10%) showed a statistically significant effect on the rats wound contraction, similar to that exerted by the reference drug Cicatryl-Bio. Moreover, A. absinthium methanol extract showed the best antibacterial activity against the Gram-negative Escherichia coli ATCC 10536 (MIC 1.25–2.5 mg/mL) and the Gram-positive Staphylococcus aureus ATCC 6538 (0.31–0.625 mg/mL). The absence of oral and topical toxicity of the treated animals allowed to establish the safety of the ointments. Overall, data collected in the present study support and validate the use of A. absinthium as a wound healing agent in the Algerian traditional medicine.

New Evidence for Artemisia absinthium L. Application in Gastrointestinal Ailments: Ethnopharmacology, Antimicrobial Capacity, Cytotoxicity, and Phenolic Profile

“…A recent study [44] of A. absinthium methanol extract has indicated MIC 2.5-1.255 mg/mL towards Escherichia coli ATCC 10536, while in our study it exhibited MIC 4 mg/mL towards E. coli (IBRS E003) resistant strain. Unlike high susceptibility of resistant P. aeruginosa strain recorded in our study, in the study by Boudjelal et al [44], it did not show activity (MIC > 2.5 mg/mL) as well as the one by Khan et al [45]. Methanolic extract studied previously by Hasannezhad et al [46] has shown MIC 41.7 mg/mL towards L. monocytogenes PTCC 1298, unlike 4 mg/mL determined in our study.…”

Artemisia absinthium L. Aqueous and Ethyl Acetate Extracts: Antioxidant Effect and Potential Activity In Vitro and In Vivo against Pancreatic α-Amylase and Intestinal α-Glucosidase

“…The values of the phenolic compound contents found in our extracts are lower than those obtained by Boudjelal et al [ 43 ] (180.33 ± 16.25 mg GAE/g DE). On the other hand, the values obtained from the present study are higher than those found by Kružinauskaitė et al [ 44 ], whose phenolic compound content of A. absinthium extracts varies from 21.19–24.74 mg GAE/g ED.…”

Potential Mechanisms Involved in the Protective Effect of Dicaffeoylquinic Acids from Artemisia annua L. Leaves against Diabetes and Its Complications

“…A methanolic extract of Artemisia annua was used for the first time for the composition of a wound dressing that was shown to increase proliferation of L929 fibroblast cells but only after 4 days of incubation and to exert potent antibacterial activity [ 43 ]. Furthermore, accelerated wound healing was described in other Artemisia species [ 44 , 45 ]. Rhimi et al (2019) has reported enhanced wound healing in mice by using an ointment of the ethanolic extract of Dittrichia viscosa , family Asteraceae [ 46 ].…”