Phytochemical profile of fresh and senescent leaves due to storage for<i>Ficus deltoidea</i>

Chua, Lee Suan; Hidayathulla, Syed

doi:10.1080/11263504.2015.1076085

Cited by 7 publications

(6 citation statements)

References 50 publications

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“…[16,17] as well as the leaf litter of Central Europe's forests [18]. According to Chua and Hidayathulla [19], fallen senescent leaves contain more amounts of phenolic compounds compared to green leaves. Public green space including urban forests and parks form a significant portion of Central Europe cities, e.g.…”

Section: Introductionmentioning

confidence: 99%

Laccase activity of the ascomycete fungus Nectriella pironii and innovative strategies for its production on leaf litter of an urban park

et al. 2020

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A laccase-producing ascomycete fungus was isolated from soil collected around the premises of a textile dye factory and identified as Nectriella pironii. Efficient laccase production was achieved via the synergistic action of 1 mM copper sulfate and ferulic acid. Extracts of rapeseed oil cake, grass hay, and leaf litter collected in a pocket urban park were used for enzyme production. The highest laccase activity (3,330 U/L) was observed in the culture grown on the leaf litter extract. This is the first report on biosynthesis of laccase by N. pironii. This is also the first study on utilization of naturally fallen park leaves as a substrate for fungal laccase production. The extracellular enzyme possessing laccase activity was purified to homogeneity by ion-exchange and gel filtration chromatographic techniques. The amino acid sequence of the protein revealed highest similarity to the laccase enzyme produced by Stachybotrys chartarum-and considerable homology to those produced by other fungal species. The purified laccase possessed a molecular mass of 50 kDa. The enzyme had an optimum pH of 2.0 or 6.0 and retained more than 50% of residual activity after 3 hours of incubation at pH 3.0-10.6 or 4.0-9.0 when 2,2 0-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid or 2,6-dimethoxyphenol, respectively, were used. Dithiothreitol, β-mercaptoethanol, and sodium azide at 1 mM concentration strongly inhibited the laccase activity, while in the presence of 50 mM urea, the enzyme was found to retain 25% of its activity. The laccase was able to decolorize more than 80% of Indigo Carmine, Remazol Brilliant Blue R, Reactive Orange 16, and Acid Red 27 dyes within 1 h. The possibility of leaf litter use for the production of the laccase enzyme from N. pironii (IM 6443), exhibiting high pH stability and degradative potential, makes it a promising tool for use in different environmental and industrial operations.

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Section: Introductionmentioning

confidence: 99%

Laccase activity of the ascomycete fungus Nectriella pironii and innovative strategies for its production on leaf litter of an urban park

et al. 2020

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“…Standardisasi dilakukan tidak hanya pada produk yang akan dibuat melainkan mulai dari bibit, budidaya, pasca panen, hingga menjadi produk. Profil fitokimia daun yang segar dan tua akibat penyimpanan telah dilaporkan oleh Chua & Hidayathulla [17]. Di sisi lain, efek tempat tumbuh terhadap kadar bahan aktif seperti flavonoid belum banyak dilaporkan.…”

Section: Pendahuluanunclassified

Tempat Tumbuh dan Kandungan Flavonoid Total Daun Tabat Barito (Ficus deltoidea Jack.)

Siswoyo¹,

Batubara²,

Aristyanti³

2016

Proc. Mul. Pharm. Conf.

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Tujuan penelitian ini ialah mengetahui perbedaan kadar bahan aktif yaitu flavonoid daun tabat barito (Ficus deltoidea Jack.) pada dua tempat tumbuh yang berbeda. Daun tabat barito dikoleksi dari Taman Nasional Gunung Gede Pangrango (TNGGP) dan Taman Nasional Gunung Halimun Salak (TNGHS). Tabat barito di TNGGP yang digunakan dalam penelitian tumbuh sebagai efifit, sedangkan di TNGHS tumbuh di atas tanah. Daun tanaman diekstraksi dengan etil asetat lalu ditentukan kadar total flavonoidnya menggunakan spektrometri. Hasil penelitian menunjukkan kadar flavonoid daun tabat barito TNGHS lebih rendah dibandingkan daun tabat barito TNGGP. Perbedaan kadar flavonoid diduga karena perbedaan kadar kimia tanah pada kedua lokasi.

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“…Antioxidant properties are well known in phenolic compounds by acting as reducing agents, free radical scavengers, or metal chelators [ 30 ]. The most abundant plant phenolics are flavonoids and phenolic acids, which have a substantial antioxidant activity both in vitro as well as in vivo [ 31 ].…”

Section: Resultsmentioning

confidence: 99%

Phytochemical Profile of the Ethanol Extract of Malvaviscus arboreus Red Flower and Investigation of the Antioxidant, Antimicrobial, and Cytotoxic Activities

et al. 2022

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Flowers are rich sources of bioactive antimicrobial, antioxidant, and anticancer components. This study aimed to determine the constituents of the ethanol extract of Malvaviscus arboreus red flower (ERF) by GC-MS analysis and HPLC identification of phenolic compounds and flavonoids, in addition to the 1HNMR fingerprint. The antimicrobial, antioxidant, and cytotoxic activities of the ERF were investigated. The GC-MS analysis revealed twenty-one components, while HPLC analysis revealed the presence of phenolic and flavonoid compounds. The ERF showed antifungal and antibacterial activity. The highest antibacterial activity was found against Vibrio damsela where a time-kill assay revealed a decline in the amount of viable V. damsela. For fungi, the highest activity was observed against Aspergillus terreus. Using the SRB test on HepG2, the anti-proliferative efficacy of the ERF was evaluated. Cell cycle analysis was utilized to determine autophagic cell death. The ERF prevented the proliferation of the HepG2 cell line with an IC50 of 67.182 µg/µL. The extract primarily promoted apoptosis in HepG2 cells by accumulating hypodiploid cells in the sub-G0/G1 phase, increased caspase 3/7 activity, and caused considerable autophagic cell death in apoptosis-deficient cells. Finally, the observed elevation of cancer cell death indicated that ERF had substantial anticancer potential against HepG2 cells.

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Phytochemical profile of fresh and senescent leaves due to storage forFicus deltoidea

Cited by 7 publications

References 50 publications

Laccase activity of the ascomycete fungus Nectriella pironii and innovative strategies for its production on leaf litter of an urban park

Laccase activity of the ascomycete fungus Nectriella pironii and innovative strategies for its production on leaf litter of an urban park

Tempat Tumbuh dan Kandungan Flavonoid Total Daun Tabat Barito (Ficus deltoidea Jack.)

Phytochemical Profile of the Ethanol Extract of Malvaviscus arboreus Red Flower and Investigation of the Antioxidant, Antimicrobial, and Cytotoxic Activities

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