Phytochemical profile, enzyme inhibition activity and molecular docking analysis ofFeijoa sellowianaO. Berg

Abstract

Feijoa sellowiana leaves and fruits have been investigated as a source of diverse bioactive metabolites. Extract and eight metabolites isolated from F. sellowiana leaves were evaluated for their enzymatic inhibitory activity against a-glucosidase, amylase, tyrosinase, acetylcholinestrerase and butyrylcholinesterase both in vitro and in silico. Feijoa leaves' extract showed strong antioxidant activity and variable levels of inhibitions against target enzymes with a strong anti-tyrosinase activity (115.85 mg Kojic acid equivalent/g). Additionally, a-tocopherol emerged as a potent inhibitor of AChE and BChE (5.40 & 10.38 mmol galantamine equivalent/g, respectively). Which was further investigated through molecular docking and found to develop key enzymatic interactions in AChE and BChE active sites. Also, primetin showed good anti BChE (11.70 mmol galantamine equivalent/g) and anti-tyrosinase inhibition (90.06 mmol Kojic acid equivalent/g) which was also investigated by molecular docking studies. HIGHLIGHTS Isolation of eight bioactive constituents from Feijoa sellowiana leaves. In vitro assays using different enzymatic drug targets were investigated. In silico study was performed to define compound interactions with target proteins. Feijoa leaf is an excellent source of anti-AChE and antityrosinase bioactives.

Cattleianal and Cattleianone: Two New Meroterpenoids from Psidium cattleianum Leaves and Their Selective Antiproliferative Action against Human Carcinoma Cells

“…EI-MS identified pseudomolecular ion [M-H] − at m/z = 397 for C 24 H 29 O 5 with fragment ions appearing at m/z 195 (95%), 161 (47%) and 203(28%), (Supplementary Data, Figure S1). 1 H-NMR δ ppm (400 MHz, CDCl S3).…”

“…Based on NMR spectra, the presence of a fully substituted phloroglucinol ring was inferred by the carbon signals (C-1 -C-6 ), while no aromatic proton was observed in the 1 H-NMR spectrum. Two phenolic substituents characteristic of phloroglucinol were readily determined in 1 H-NMR at δ H 12.96 (s, 1H) and 13.22 (s, 1H), which showed multiple HMBC correlations to aromatic carbons at δ C 166.8, 166.9, 103.1 and 104.7, (Supplementary Data, Figure S4). Additionally, the presence of 3,5 formyl substitutions was established by the two carbon signals (C-7 and C-8 ) and aldehydic proton signals at δ H 9.92 (s, 1H) and 10.11 (s, 1H) which showed HMBC correlations to C-1 , C-3 , C-4 and C-6 (Supplementary Data, Figure S4), establishing the 3,5 formyl phloroglucinol moiety characteristic for Psidium species [13][14][15].…”

“…Mass spectra were obtained using Varian Mat. 711, Finningan SSQ 7000 and OMM 7070 E. 1 H, and 13 C-NMR analyses were recorded on a Bruker AVIIIHD400 FT-NMR Spectrometer (400/3) instrument (Japan). Optical activity was estimated with Kruss polarimeter.…”

“…Myrtaceous plants exert a myriad of biological activities including anti-diabetic, cytotoxic, anti-inflammatory and anti-bacterial biofilm activities [1][2][3]. Psidium cattleianum Sabine (Family Myrtaceae) is a shrub or small tree native to Brazil, where it is known as "araçá" [4].…”

Extraction, identification and molecular docking studies evaluation of Momordica tuberosa (Roxb) against Anopheles

“…This kind of study, phytochemical against enzyme inhibition done by various groups, Nwakulite groups describe the co ligands inhibits the amylase activity and control the glucose metabolism (26) . Fatema R. Saber group studied the phytochemical interaction with active site amino acid residue of BChE and ACE enzymes by a mixture H-bonding, and hydrophobic forces (27) . The AnoACE2 is an important biological growth enzyme for mosquito.…”