Phytochemical Profile and Biological Activities of Helianthemum canum l. baumg. from Turkey

Abstract

In the current study, antioxidant, antibacterial activities, and the phenolic compositions of extracts from Helianthemum canum L. Baumg. (Apiaceae) aerial parts were investigated for the first time. The H. canum was extracted with 70% methanol (HCMeOH) and water (HCW). Both extracts were determined by total phenolic contents (3 mg/ml), flavonoids (1.5 mg/ml), flavonols (1.5 mg/ml), qualitative-quantitative compositions, iron (II) chelation activities (0.1 - 5 mg/ml), free radical scavenging activities (DPPH : 0.01 - 0.6 mg/ml and ABTS : 0.125 - 0.5 mg/ml) and the effect upon inhibition of β-carotene/linoleic acid co-oxidation (1 mg/ml). The peroxidation level was also determined using the thiobarbituric acid method (0.01 - 1.5 mg/ml). The results of the activity tests given as IC values were estimated from non-linear algorithm and compared with standards. Antibacterial activities of extracts and standards were evaluated against Gram-negative and -positive ten standard strains using disc diffusion and broth microdilution methods. The MIC results (312.5 - 2500 μg/ml) against tested microorganisms varied from 625 to 2500 μg/ml. In HPLC analysis, 3,5-dihydroxybenzoic acid was found as the main substance in both extracts. These results showed that HCMeOH was richer in phenolic compounds (284.13 ± 0.30 mg GAE/g extract) from HCW (244.55 ± 0.35 mg GAE/g extract) In conclusion, H. canum extracts showed in vitro antibacterial and antioxidant activities.

The Wound‐Healing Potential of the Endemic Plant Helianthemum canum (L.) Baumg: Preclinical Studies Supported with Phytochemical Profiling

“…In our previous HPLC analysis of Helianthemum canum extracts, we detected several compounds including gallic acid, 3,5‐dihydroxy‐benzoic acid, 3,4‐dihydroxybenzoic acid, methyl gallate, 4‐hydroxybenzoic acid, apigenin‐7‐O‐glucoside, and luteolin‐7‐O‐glucoside. The quantities of these compounds were measured, and the primary compound, 3,5‐dihydroxybenzoic acid, was quantified at 6.669 and 23.088 mg/g in methanol and water extracts, respectively [18] …”

“…The quantities of these compounds were measured, and the primary compound, 3,5-dihydroxybenzoic acid, was quantified at 6.669 and 23.088 mg/g in methanol and water extracts, respectively. [18] Sulphorhodamine B (SRB) and RTCA assay Fibroblasts are the main epithelial cells that affect the number of extracellular matrix components. [3] When a tissue is damaged, fibroblasts migrate there and start to produce extracellular matrix proteins.…”

Phytochemical and Biological Investigation of Helianthemum nummularium, a High-Altitude Growing Alpine Plant Overrepresented in Ungulates Diets

“…Except for p-hydroxybenzoic acid (1) and plantainoside B (8), the remaining compounds are flavonoid derivatives. Compounds 1-5 were already identified in species of the genus Helianthemum, including Helianthemum canum, Helianthemum sessiflorum, Helianthemum glomeratum, and Helianthemum ruficomum [14][15][16][17][18]. However, quercetin-3-Oglucoside (6), quercetin-3-O-glucuronide (7), and plantainoside B (8) have never been reported in any species of Helianthemum [19].…”