Phytochemical Profile and Antimicrobial Potential of Extracts Obtained from Thymus marschallianus Willd

Abstract

Thymus marschallianus Willd. is a Lamiaceae species spread in a large variety of habitats worldwide. The aim of the present research was to analyse two different samples belonging to this species, one obtained from the spontaneous flora and one from culture. The total polyphenols, flavonoids, and phenolic acid contents were spectrophotometrically determined. Qualitative and quantitative analysis of polyphenols was performed by an HPLC-DAD-ESI (+)-MS method. For the antibacterial assay, the well-diffusion and the broth microdilution methods were used. Analysis of polyphenols revealed for both samples the presence of flavonoids like luteolin, quercetin, apigenin and their derivatives, but also of rosmarinic acid and methyl-rosmarinate. Differences regarding the amount of these compounds were emphasized. Significantly larger amounts of flavonoids were found for the sample harvested in the spontaneous flora, while for the rosmarinic acid, larger amounts were found for the cultured sample. Both samples displayed promising antibacterial activity, particularly towards Gram positive organisms. T. marschallianus represents, therefore, a rich source of polyphenolic compounds that prove its promising potential as a medicinal species.

Targeting Oxidative Stress Reduction and Inhibition of HDAC1, MECP2, and NF-kB Pathways in Rats With Experimentally Induced Hyperglycemia by Administration of Thymus marshallianus Willd. Extracts

“…979, from which sample TMC was obtained). The taxonomic identification of the species was performed by Nina Ciocârlan, PhD, from the Botanical Garden of the Moldavian Science Academy, where voucher specimens are deposited in the Herbarium ( Niculae et al, 2019 ).…”

“…Thymus (thyme) is one of the most important genus belonging to the Lamiaceae family (Niculae et al, 2019). This family is known worldwide for its traditional uses, in the treatment of different pathologies, being also used since ancient times for their importance as aromatic plants (Nabavi et al, 2015), that are assigned to their composition in essential oils (Tohidi et al, 2019) and phenolic compounds (Raudone et al, 2017).…”

“…Besides the important number of volatile compounds, the species is also known for its composition in phenolic compounds, which are also responsible for its biological activity (Raudone et al, 2017). Among these compounds, the most important appears to be rosmarinic acid (RA), which is found in large amounts in the flowering aerial parts of the species (Niculae et al, 2019). This phenolic acid is responsible for numerous protective biological activities (Nunes et al, 2017), including the antidiabetic potential and antioxidant capacity (Nunes et al, 2017;Ngo et al, 2018;Nadeem et al, 2019).…”

Evaluation of Hepatoprotective Activity and Oxidative Stress Reduction of Rosmarinus officinalis L. Shoots Tincture in Rats with Experimentally Induced Hepatotoxicity

“…Among the most important flowering plant families, the Lamiaceae family is one of the largest, comprising numerous species that are known for their biological activities or for their use in different branches related to economy [ 1 , 2 , 3 ]. Species belonging to the genera Salvia [ 4 ], Melissa [ 5 ] or Thymus [ 6 ] are the most well known, being widely used in different forms for the treatment of numerous pathologies, but also for their nutritional or economical values [ 4 , 5 , 6 ].…”

Dracocephalum heterophyllum (DH) Exhibits Potent Anti-Proliferative Effects on Autoreactive CD4+ T Cells and Ameliorates the Development of Experimental Autoimmune Uveitis

“…HPLC analysis revealed that this extract is composed of six main components. Most of them have the anti-oxidation, anti-inflammatory, antibacterial, and antitumor effects (23)(24)(25)(26)(27)(28)(29). Interestingly, three of them (methyl Rosmarinate, Luteolin and Diosmetin) are capable of suppressing cell proliferation.…”