Phytochemical profile and anthelmintic effects of Laurus nobilis essential oil against the ovine nematode Haemonchus contortus and the murine helminth model Heligmosomoides polygyrus

Sebai, Essia; Abidi, Anouar; Benyedem, Hayet; Dhibi, Mokhtar; Hammemi, Ines; Akkari, Hafidh

doi:10.1016/j.vetpar.2022.109835

Cited by 7 publications

(7 citation statements)

References 25 publications

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“…Meanwhile, resistance cases are a major problem in the development of ruminants due to the use of chemical anthelmintic drugs. One effort to deal with anthelmintic resistance is the use of natural anthelmintics [6]. The bark of Paraserianthes falcataria has been confirmed to have active compounds that succeed in killing H. contortus in vitro, and when observed using SEM, it shows that there is damage to the cuticle structure [7]; the same results have also been found in the fruit peel of Carica pubescens.…”

Section: Introductionmentioning

confidence: 76%

In vivo study: The effects of Carica pubescens seed extract on the anthelmintic activity, feed digestibility, performance, and clinical parameters of thin-tailed sheep

Baihaqi,

Sofyan,

Suwignyo

et al. 2024

IOP Conf. Ser.: Earth Environ. Sci.

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This research serves to follow up on the results of an in vivo study of the administration of aqueous seed extract (ASE) of Carica pubescens waste on the performance and health parameters of thin-tailed sheep. This research was conducted at Al Baihaqi farm in Kediri, East Java. Thirty-two thin-tailed sheep aged 12 months were randomly given four treatments with P0 = negative control, P1 = 2.5% ASE, P2 = 5% ASE, and P4 = positive control (Albendazole). This study used sheep that were naturally exposed to Haemonchus contortus (from the farmer’s field) with the epg qualification being above 1000. The in vivo study was carried out for 9 weeks with the administration of Carica seed extract on the initial 7 days in a row, and the positive control was given Albendazole once on the seventh day. The number of ASE grants was based on the average fluid in the abomasum per kg of body weight. The feed design used in this study was wheat bran and Pennisetum purpureum cv. Mott according to the livestock’s needs. The data taken in this study were the feed consumption, digestibility, average daily gain, percent fecal egg count reduction, body condition score, and clinical parameters. The results showed that the FECR, ADG, and clinical parameters (AST and ALT) (P>0.05) underwent significant changes to support good performance while the BUN, creatinine, feed consumption, and digestibility were normal. This study indicates that ASE of up to 5% can be used as an anthelmintic alternative and support the recovery of sheep performance.

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Section: Introductionmentioning

confidence: 76%

In vivo study: The effects of Carica pubescens seed extract on the anthelmintic activity, feed digestibility, performance, and clinical parameters of thin-tailed sheep

Baihaqi,

Sofyan,

Suwignyo

et al. 2024

IOP Conf. Ser.: Earth Environ. Sci.

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“…These results agree partly with previous research https://doi.org/10.1007/s42452-024-05835-z conducted in Brazil, regarding low concentrations of cumin EO (0.35 to 9.4 mg/mL) provoked high rates of mortality (93% to 98%) on the ruminants gastrointestinal nematode Haemonchus contortus [9], and even against the eggs of the liver trematode Fasciola hepatica [24]. Full (100%) ovicidal effect on H. contortus eggs at a dose of 1.72 mg laurel EO/mL has been demonstrated [34].…”

Section: Discussionmentioning

confidence: 99%

Preliminary screening of plant essential oils from an oceanic climate zone (NW Spain) for the control of equine cyathostomins

Garduño,

Vázquez,

Monteiro

et al. 2024

Discov Appl Sci

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Cyathostomins (Strongylida: Cyathostominae) are gastrointestinal nematodes (GIN) that affect equines in many parts of the world. Due to anthelmintic resistance (AR), it is necessary to search for control alternatives. The objective of the study was to carry out a screening of 26 essential oils (EOs) from plants to determine in vitro their action on cyathostomins. Essential oils were obtained by hydro-distillation from leaves, flowers, fruits, and seeds of the selected species and used against the eggs of cyathostomins by means of the tests of egg hatching and larval motility inhibition. For each EO, different concentrations were prepared (250, 125, 62.5, 31.3, 15.6, 7.8, 3.9, 2.0, 1.0, 0.5 and 0.24 mg/mL). Oxfendazole at 2.5% mixed with closantel 5% was used as positive control, and PBS served as negative control. Cyathostomin eggs were obtained from feces of infected horses and infective larvae were recovered after coproculture. The in vitro test was carried out 36 h after the challenge with the EOs. The highest production of EOs was obtained from leaves of bay, eucalyptus, mallow, and basil, as well as from seeds of black pepper, coriander, cinnamon, anise, and peel of orange and cloves, cumin, and Lawson’s cypress. The oils extracted from laurel leaves, ground cinnamon, anise, cumin, and coriander seeds had a very high effect on cyathostomins eggs up to concentrations lower than 3.9 mg/mL. Therefore, in addition to the uses against other pathogens such as fungi and bacteria, some EOs might attain great importance as an alternative control strategy in the control of nematode eggs.

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“…This study is consistent with many other studies that verified the effectiveness of L. nobilis leaves against various parasites. Sebai et al (2022), found in vivo anthelmintic potential in L. nobilis extract eliminated the egg output of Heligmosomoides polygyrus after 7 days of treatment and with a 79.2% reduction in total worm counts. In addition, the presence of linalool in the Cinnamomum camphora plant markedly reduced the recovered schistosomulum from mouse skin after challenge infection.…”

Section: In Vivomentioning

confidence: 95%

“…It decreased the worm burden in infected animals (Batiha et al, 2018). The L. nobilis plant was rich in linalool, which plays a vital role in eliminating worms in the intestines of infected mice (Sebai et al, 2022). Reported that L. nobilis has potent antioxidant, antimicrobial and antibacterial activity (Kivçak and Mert, 2002).…”

Section: In Vivomentioning

confidence: 99%

The Assessment of the Anthelmintic Activity of Laurus nobilis Extract in Mice Naturally Infected with Aspiculuris tetraptera

Mares,

Murshed,

Aljawdah

et al. 2023

IJAR

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Background: The safety of laboratory mice plays an important role in the success of laboratory experiments for correct and accurate results. Parasites are one of the most common diseases that affect most organisms. The worm Aspiculuris tetraptera is a common intestinal parasite of Mus musculus and rats, it is spread around the world. Aspiculuris tetraptera infection remains a problem for modern research groups; They should be excluded and monitored in rat populations due to their effects on animal health. Methods: The present study was conducted to assess the anthelmintic activity of Laurus nobilis extract in mice naturally infected with Aspiculuris tetraptera. Used was Thirty-six adult male and female (C57BL/6) mice, naturally infected with Aspiculuris tetraptera, were divided into six groups, each comprising six mice: Group 1, was the negative control (infected, untreated), and Groups 2, 3, 4, and 5 were treated with 50, 100, 200 and 400 ìg/ml of L. nobilis for 5 days respectively. Group 6 was treated with 10 mg/mL Albendazole for 3 days as a positive control. Different six treatments were used to test Aspiculuris tetraptera worms in vitro, Containing 4 concentrations (50, 100, 200 and 400 mg/ml) of L. nobilis extract. Ten actively moving adult worms were then placed in each petri dish at room temperature. A saline solution and 10 mg/ml of Albendazole were prepared and used as negative and positive controls. After treatment, observations were made by recording the death time for worms at 20, 40, 80, 120 and 180 minutes. Worms are considered dead if they do not move for 30 seconds after touching their body parts using a surgical needle and the petri dish is shaken. Result: The analysis of phytochemicals by FT-IR for alcoholic extracts of L. nobilis extracts revealed the presence of 15% effective chemical ingredients responsible for killing worms’ activity. In vitro, worms died in 20, 40, 80, 120 and 180 minutes. Attained 96% and 100% after 180 minutes at the highest concentrations (200 and 400 mg/ml). At the same time, the untreated group lasted for long hours. The effects of the plant extract (Laurus nobilis) on Aspiculuris tetraptera worms were studied in vivo at therapeutic doses of 50, 100, 200 and 400 mg/mL. and the concentration of 400 ml/kg showed the most lethal effects for worms, infected mice that did not receive drugs were compared with the highest concentration of the extract and the reference treatment. The attention showed significant differences P≤0.05. The results showed that the mortality rate of worms taken from the intestines of the treated mice that were slaughtered three and six sdays after giving the treatment reached 96% and 100% in the concentration of 400 ml/kg of the extract and 89% and 97%for the mice that treated in 10 ml/kg of met Albendazole respectively. This research showed that herbal remedies could lead to new parasitic disease drugs, and their derivatives can be used for medication production and bioactivity improvement.

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Phytochemical profile and anthelmintic effects of Laurus nobilis essential oil against the ovine nematode Haemonchus contortus and the murine helminth model Heligmosomoides polygyrus

Cited by 7 publications

References 25 publications

In vivo study: The effects of Carica pubescens seed extract on the anthelmintic activity, feed digestibility, performance, and clinical parameters of thin-tailed sheep

In vivo study: The effects of Carica pubescens seed extract on the anthelmintic activity, feed digestibility, performance, and clinical parameters of thin-tailed sheep

Preliminary screening of plant essential oils from an oceanic climate zone (NW Spain) for the control of equine cyathostomins

The Assessment of the Anthelmintic Activity of Laurus nobilis Extract in Mice Naturally Infected with Aspiculuris tetraptera

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