Phytochemical Investigation, Antitumor Activity, and Hepatoprotective Effects of Acrocarpus fraxinifolius Leaf Extract

Abstract

Preclinical Research Nine known phenolic compounds were isolated from an aqueous methanolic extract of Acrocarpus fraxinifolius Weight and Arn leaves (AFL) family Fabaceae. This extract of AFL contained approximately 169 mg gallic acid/g as assessed by HPLC. The AFL extract had marginal antitumor activity (IC > 200 µL/mL) but showed a concentration-dependent hepatoprotective effect against CCl -induced hepatotoxicity in vitro. Cell viability was increased, ALT and AST activity declined and reduced GSH concentration and SOD activity were restored as compared with silymarin. In vivo concurrent administration of AFL extract (500 mg/kg po) showed a hepatoprotective effect against gamma irradiation and CCl as evidenced by reduction of TNF-α, interleukin-6, malondialdehyde, nitric oxide, DNA fragmentation, caspase-3 activity, and downregulation of its m-RNA level and decreased proapoptotic protein Bax expression. AFL extract enhanced glutathione peroxidase, superoxide dismutase, and catalase activities, reduced glutathione concentrations and upregulated the expression of antiapoptotic Bcl-2. The extract could ameliorate hepatic injuries induced by gamma irradiation and CCl in rats suggesting potent hepatoprotective activity. Drug Dev Res 78 : 210-226, 2017. © 2017 Wiley Periodicals, Inc.

SIRT1 Activation Enhancing 8,3′-Neolignans from the Twigs of Corylopsis coreana Uyeki

“…The water residues were subjected to Diaion HP-20 resin column chromatography and eluted with H 2 O and MeOH to provide two fractions. The CHCl 2 , EtOAc and MeOH extracts were separated by chromatographic methods, and three previously undescribed neolignans ( 1 – 3 ) were isolated along with 13 known compounds including bergenin ( 4 ) [ 18 ], 11- O -galloyl bergenin ( 5 ) [ 19 ], (−)-epicatechin ( 6 ) [ 20 ], (−)-epicatechin 3- O -gallate ( 7 ) [ 21 ], (−)-epigallocatechin gallate ( 8 ) [ 22 ], 1,2,4,6-tetra- O -galloyl-β-D-glucose ( 9 ) [ 23 ], 1,2,3,4,6-penta- O -galloyl-β-D-glucose ( 10 ) [ 24 ], ellagic acid ( 11 ) [ 25 ], benzoic acid ( 12 ) [ 26 ], 3,4,5-trihydroxybenzoic acid ( 13 ) [ 27 ], 3,5-dihydroxy-4-methoxybenzoic acid ( 14 ) [ 28 ], 1-(4-hydroxy-3,5-dimethoxyphenyl)-1-propanone ( 15 ) [ 29 ] and 3,4,5-trimethoxyphenyl-(6′- O -galloyl)- O -β-D-glucopyranoside ( 16 ) ( Figure 1 ) [ 30 ]. The structures of the known compounds ( 4 - 16 ) were identified by comparison with previously reported NMR data.…”

Pink Cedar (Acrocarpus fraxinifolius): its prophylactic role against APAP–induced organs toxicity in rats and its antiviral activity against Herpes simplex virus type 1

“…Also, these modulations led to alleviate the loss in body weight gain [2] and relative kidney weight. This may be attributed to the fact that phenolic acids in AFL have anti-oxidant and free radical chelators/scavenger'sactivities as with special impact over hydroxyl/peroxyl radicals, superoxide anions, and peroxynitrites [18].…”

“…Recently, bioactive phenolics such as brevifolin carboxylic acid, ellagic acid, gallic acid and methyl gallate were identified from the extract of AFL [18,20], and may be responsible for its radical scavenging activity [50].…”

“…Also, it is distributed in the tropical countries including Egypt. It common name pink cedar, mundani or shingle tree [2,18]. The extracts of the plant were reported to have anti-oxidant, anti-diabetic, anti-proliferative, anti-inflammatory, and hepatoprotective activities in vivo [2,[19][20][21][22][23] as well as antitumor activity in vitro [18] [.…”

Acrocarpus fraxinifolius Wight and Arn. Bark; phenolics, toxicity studies, antioxidant and anti-inflammatory activities

“…The variation of phenolic contents of older and younger tree of the plant barks together with its in vitro antioxidant activities was reported (et al, 2015). Considering the previous reported work for investigation of phenolics, lipoidal contents along with different biological activities of A. fraxinifolius (Abou Zeid et al, 2011; as well as phenolic, tannins, flavonoids, in vivo hepatoprotective, antiproliferatives, antioxidant and antimicrobial activities of A. fraxinifolius leaves were studied (El-Nashar et al, 2017;Walaa et al, 2016). A flavone glycoside was isolated from the seeds (Bardia et al, 2005) of A. fraxinifolius , which was subjected to amino acid analysis (Bardia, Rao 2004a, b).…”