Phytochemical Investigation, Antimicrobial, Antioxidant and Anticancer Activities of Acer cappadocicum Gled

Abstract

The appearance of novel microbial resistance, diverse cancer ailment and several other morbidities such as appetite loss, hair loss, anemia, cell damage, etc., are among most critical situation that keeps the phytochemical quest on. Thus, this study characterized the antimicrobial, antioxidant, and anticancer potentials of a rarely accessed Acer cappadocicum gled (AC) population thriving in a remote Palas Valley in northern Pakistan. Leaf extracts of the plant were prepared in organic solvents with different polarities through maceration. Extracts were subjected to antimicrobial, antioxidant, and anticancer activities using agar well, DPPH and cell viability assays. A. cappadocicum methanolic extract (ACM) significantly inhibited bacterial growth, followed by n-butanolic extract (ACB) with the second-highest bacterial inhibition. Similar activity was observed against mycelial growth inhibition in plant-fungal pathogen by ACM and ACB. However, human pathogenic fungi did not affect much by extracts. In antioxidant assessment, the chloroform extract (ACC) showed strong scavenging activity and in cytotoxic evaluation, extracts restricted growth proliferation in cancer cells. The inhibitory evidence of extracts, potent scavenging ability, and low cell viability of human-derived cell lines supports the antimicrobial, antioxidant and anticancerous potential of A. cappadocicum. It advances our quest for natural product research.

Evaluation of Antimicrobial and Anticancer Activities of Selected Medicinal Plants of Himalayas, Pakistan

“…Several active compounds have been extracted and isolated to confirm the traditional uses of plants. Hence, there is growing interest in validating the biological potential of unusual plants, which further facilitates herbal medicine research [ 14 ]. The medicinal properties of Prunus and Quercus species are reported in various studies, including as diuretic, anti-inflammatory, antipyretic and refrigerant, astringent, heart disease protection, antibacterial hepatoprotective, antidiabetic, anticancer, gastroprotective, antioxidant, and cytotoxic activities [ 15 , 16 ].…”

“…The methanolic extracts of selected plants were subjected to the preliminary screening of phytochemicals such as alkaloids, flavonoids, saponins, tannins, and carbohydrates [ 14 , 27 , 28 , 29 , 30 ].…”

Extracellular Matrix Optimization for Enhanced Physiological Relevance in Hepatic Tissue-Chips

“…88065) derived from human hepatoblastoma was purchased from Korean Cell Line Bank, Korean Cell Line Research Foundation, Seoul, South Korea. The immortal HepG2 cells were cultured in 10% fetal bovine serum containing DMEM cell culture media supplemented with 1% v / v penicillin and streptomycin (P/S) antibiotic solution and incubated at 37 °C with 5% CO 2 [ 19 ]. Before starting the experiment, glass chips were sterilized using 70% ethanol and a subsequent ultraviolet exposure for 1 h.…”

Study of the Anticancer Potential of Plant Extracts Using Liver Tumor Microphysiological System

“…Indigenous Himalayan inhabitants reported ethnobotanical uses of Acer cappadocicum Gled against several diseases, such as rheumatoid arthritis, fatty liver disease, conjunctivitis, and inflammation. Additionally, several bioactivity analyses have confirmed the ethnomedical significance of Acer species towards hepatoprotective function [16][17][18][19][20][21]. Previously, the study of the phytochemical evaluation, antimicrobial activities, and cytotoxicity of Acer cappadocicum Gled extracts forecasted their potential for drug discovery [19].…”

“…Additionally, several bioactivity analyses have confirmed the ethnomedical significance of Acer species towards hepatoprotective function [16][17][18][19][20][21]. Previously, the study of the phytochemical evaluation, antimicrobial activities, and cytotoxicity of Acer cappadocicum Gled extracts forecasted their potential for drug discovery [19]. Hence, the methanolic extracts of Acer cappadocicum Gled were chosen to evaluate further the anticancer potential of Acer cappadocicum Gled using a liver tumor MPS.…”