Phytochemical Investigation and Antioxidant Activity of Leaf Extract of Withania somnifera from Konso, South Ethiopia

Simur, Tesfaye Tebeka

doi:10.13005/ojc/3404016

Cited by 4 publications

(1 citation statement)

References 18 publications

(16 reference statements)

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“…From Table 2 , it can be seen that Withania frutescens and Withania somnifera possessed antioxidant activities. Notably, the total antioxidant capacity of RWA was 760 ± 10 µg AAE/mg ( Figure 2 ), which is higher than those recorded for root extracts (478 ± 38 µg AAE/mg) and leaf extracts (317 ± 47 µg AAE/mg) of Withania frutescens [ 40 ], and even higher than that recorded for the extract from Withania somnifera (45.41 ± 0.018 µg AAE/mg) [ 51 ]. Antioxidant activity of B-carotene revealed that the free radical scavenging activity of RWA was 81.4% ± 1.1%, and 90.1% ± 1.4% for quercetin ( Figure 2 ), which is higher than those recorded for the root extract (57%) and leaf extract (36%) of Withania frutescens [ 50 ].…”

Section: Resultsmentioning

confidence: 73%

Promising Antioxidant and Antimicrobial Potencies of Chemically-Profiled Extract from Withania aristata (Aiton) Pauquy against Clinically-Pathogenic Microbial Strains

Alzahrani

2022

Molecules

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Withania aristata (Aiton) Pauquy, a medicinal plant endemic to North African Sahara, is widely employed in traditional herbal pharmacotherapy. In the present study, the chemical composition, antioxidant, antibacterial, and antifungal potencies of extract from the roots of Withania aristata (Aiton) Pauquy (RWA) against drug-resistant microbes were investigated. Briefly, RWA was obtained by maceration with hydro-ethanol and its compounds were identified by use of high-performance liquid chromatography (HPLC). The antioxidant activity of RWA was determined by use of ferric-reducing antioxidant power (FRAP), 1,1-diphenyl-2-picrylhydrazyl (DPPH), and total antioxidant capacity (TAC). The evaluation of the antimicrobial potential of RWA was performed against drug-resistant pathogenic microbial strains of clinical importance by use of the disc diffusion agar and microdilution assays. Seven compounds were identified in RWA according to HPLC analysis, including cichoric acid, caffeic acid, apigenin, epicatechin, luteolin, quercetin, and p-catechic acid. RWA had excellent antioxidant potency with calculated values of 14.0 ± 0.8 µg/mL (DPPH), 0.37 ± 0.08 mg/mL (FRAP), 760 ± 10 mg AAE/g (TAC), and 81.4% (β-carotene). RWA demonstrated good antibacterial potential against both Gram-negative and Gram-positive bacteria, with inhibition zone diameters ranging from 15.24 ± 1.31 to 19.51 ± 0.74 mm, while all antibiotics used as drug references were infective, except for Oxacillin against S. aureus. Results of the minimum inhibitory concentration (MIC) assay against bacteria showed that RWA had MIC values ranging from 2.13 to 4.83 mg/mL compared to drug references, which had values ranging from 0.031 ± 0.003 to 0.064 ± 0.009 mg/mL. Similarly, respectable antifungal potency was recorded against the fungal strains with inhibition zone diameters ranging from 25.65 ± 1.14 to 29.00 ± 1.51 mm compared to Fluconazole, used as a drug reference, which had values ranging from 31.69 ± 1.92 to 37.74 ± 1.34 mg/mL. Results of MIC assays against fungi showed that RWA had MIC values ranging from 2.84 ± 0.61 to 5.71 ± 0.54 mg/mL compared to drug references, which had values ranging from 2.52 ± 0.03 to 3.21 ± 0.04 mg/mL. According to these outcomes, RWA is considered a promising source of chemical compounds with potent biological properties that can be beneficial as natural antioxidants and formulate a valuable weapon in the fight against a broad spectrum of pathogenic microbes.

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Section: Resultsmentioning

confidence: 73%

Promising Antioxidant and Antimicrobial Potencies of Chemically-Profiled Extract from Withania aristata (Aiton) Pauquy against Clinically-Pathogenic Microbial Strains

Alzahrani

2022

Molecules

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In vitro antioxidant activity and polyphenolic content of commonly used spices from Ethiopia

Sasikumar

Erba

Egigu

2020

Heliyon

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Introduction In this study, the antioxidative effectiveness, and polyphenolic content of methanol and aqueous extracts of spices such as Lippia adoensis (Koseret), Nigella sativa (Thikur azmud), Piper capense (Timiz), Thymus schimperi (Tosign) and Trachyspermum ammi (Netchazmud), consumed among people of Ethiopia were investigated. Methods The antioxidant activity was assessed via established in vitro assay models such as 2, 2-Diphenyl-1-Picrylhydrazyl (DPPH) radical quenching assay, reducing power assay and reactive nitrogen species (RNS) inhibitory potential. Total phenolics content was measured according to Folin-Ciocalteu's method and total flavonoid content was estimated by using Aluminium chloride colorimetric method. Results The results showed that the total phenolic content was highest in both methanol (720 ± 0.04 mg GAE/100 g extract DW) and aqueous (580 ± 0.08 mg GAE/100 g DW) extracts of L. adoensis . Among the five tested spices, the methanol and aqueous extracts of L. adoensis exerted the strongest DPPH radical quenching activity with IC 50 values of 49.17 ± 1.26 and 20.99 ± 8.6 μg/mL, respectively. Both methanol and aqueous extracts of L. adoensis showed notable reducing capacity. The highest RNS scavenging activity was shown by both methanol (IC 50 597.21 ± 6.99 μg/mL) and aqueous (IC 50 = 551.5 ± 28.9 μg/mL) extracts of L. adoensis . High to moderate positive correlations were observed between total phenolic contents and in vitro antioxidant assays. This indicates that the antioxidant activities of the tested spices are attributed to the phenolic contents. Conclusion The results of the present work revealed that the tested spices demonstrated high phenolic contents and antioxidant properties. Thus, these spices are worth considering as important sources of natural antioxidant agents.

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Phytochemical analysis of Withania somnifera leaf extracts by GC-MS and evaluating antioxidants and antibacterial activities

Ezez

Mekonnen

Tefera

2023

International Journal of Food Properties

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Phytochemical Investigation and Antioxidant Activity of Leaf Extract of Withania somnifera from Konso, South Ethiopia

Cited by 4 publications

References 18 publications

Promising Antioxidant and Antimicrobial Potencies of Chemically-Profiled Extract from Withania aristata (Aiton) Pauquy against Clinically-Pathogenic Microbial Strains

Promising Antioxidant and Antimicrobial Potencies of Chemically-Profiled Extract from Withania aristata (Aiton) Pauquy against Clinically-Pathogenic Microbial Strains

In vitro antioxidant activity and polyphenolic content of commonly used spices from Ethiopia

Phytochemical analysis of Withania somnifera leaf extracts by GC-MS and evaluating antioxidants and antibacterial activities

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