Phytochemical Evaluation of Some Medicinal Plants of Puthan District of Nepal

Abstract

Different parts of eleven medicinal plants used in the traditional medicine in Puthan district were collected. Three different extracts, methanol, 50% aqueous methanoland 70% aqueous acetone extractswere prepared for each plant material. The methanol extractswere screened for the presence of different classes of phytochemicals. Total phenolic andflavonoid content, as well as DPPH freeradical scavenging activity, were evaluated. All the investigated extracts contain a high amount of phenolics and flavonoids.The highest amount of phenolicsand flavonoids were detected in the methanolextract of bark of Bauhinia variegate(355.35+3.69 mg GAE/g and 209.23 ±1.25 mg QE/g extract). Among the eight tested extracts, the highest radical scavenging activity was shown by methanol extract of bark of B. variegate (IC50 3.68 μg/ml). The extract having the highest phenolic and flavonoid content showed the lowest IC50 demonstrating the positive correlation between radical scavenging activity and total phenolic and flavonoid content.

Assessment of toxic effect ofCentella asiaticaextract and its application as natural preservative in fresh‐cut mango, pear and cabbage

“…Our previous preliminary study had proved that C. asiatica extract could exhibit a broadspectrum antimicrobial capability against different microorganisms and the association of total phenolic content (TPC) and total flavonoid content (TFC) with the antioxidant ability of C. asiatica using 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay . Several published studies have supported that phenolic content and flavonoid were associated with strong antimicrobial and antioxidant activity in different fruit or plant extracts (Ali et al, 2014;Desa et al, 2021;Edgar, 2014;Giri & Rajbhandari, 2020;Hossain et al, 2018;Panathula et al, 2014;Polash et al, 2017;Ramli, Wong, et al, 2020).…”