Phytochemical evaluation and HPTLC investigation of bark and extracts of Rhamnus Frangula Linn

Ţebrencu, Carmen Elena; Cretu, Ruxandra; Mitroi, Gabriela; Iacob, Elena; Ionescu, Elena

doi:10.1007/s11101-015-9410-8

Cited by 12 publications

(9 citation statements)

References 8 publications

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“…The HPTLC chromatographic assay was done according to literature [15,16] using a CAMAG LINOMAT IV, TLC 3 Scanner and a WINCATS Planar Chromatography Manager software. A HPTLC plate G60 F254, 200x100 mm (Merck, Germany), saturated in methanol, was used as stationary phase.…”

Section: Qualitative Assays Chromatographic Identification Of Polyphementioning

confidence: 99%

Contributions on Spruce Bark Polyphenols Identification Using Instrumental (UV-VIS Spectrometry), Qualitative (Thin Layer Chromatography) and Quantitative (HPTLC Densitometry) Methods

Pătrăuţanu¹,

Ciupercǎ²,

Popa³

et al. 2020

Rev. Chim.

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This work assessed the qualitative and quantitative polyphenols profile in a crude ethanol extract separated from spruce bark (two fractions with <0.25 and 1 mm diameter) using ultrasound assisted extraction (UAE) as a green extraction method. The evaluation of phenolic acids and condensed tannins profile was performed using instrumental (UV-VIS spectrometry), qualitative (Thin Layer Chromatography, TLC) and quantitative (HPTLC densitometry) methods. Using ultrasound assisted extraction (UAE) technique under specific parameters, the higher total phenolic content (TPC) was 29.785 mg GAE/g -1 for spruce bark fraction with particle size <0.25 mm and 14.448 mg GAE/g -1 for fraction with 1 mm diameters. The TLC assay of the crude ethanol extract was performed considering the standards: gallic, sinapic, p-coumaric and vanillic acids, catechin, epicatechin and tannic acid. Smaller material particle sizes lead to higher yield of polyphenols in ethanol extract. The quantitative evaluation by HPTLC densitometry revealed following amounts: sinapic acid 0.84 mg/g, p-coumaric acid 0.61 mg/g, catechin 1.03 mg/g, tannic acid 2.81 mg/g. Considering the chemical composition, the spread and availability, spruce bark could be considered as a resource with environmental and economic benefits.

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Section: Qualitative Assays Chromatographic Identification Of Polyphementioning

confidence: 99%

Contributions on Spruce Bark Polyphenols Identification Using Instrumental (UV-VIS Spectrometry), Qualitative (Thin Layer Chromatography) and Quantitative (HPTLC Densitometry) Methods

Pătrăuţanu¹,

Ciupercǎ²,

Popa³

et al. 2020

Rev. Chim.

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“…The HPTLC assay was performed according to Patil and collaborators, 23 as well as Țebrencu and collaborators, 24 using a CAMAG LINOMAT IV TLC 3 Scanner and WINCATS Planar Chromatography Manager software. A HPTLC plate G60 F254, 200 x 100 mm (Merck, Darmstadt, Germany) saturated in methanol was used as stationary phase for identifying phenolic acids and tannins.…”

Section: Identification Of Polyphenols Using High-performance Thin-lamentioning

confidence: 99%

Influence of Particle Size and Size Distribution on Kinetic Mechanism of Spruce Bark Polyphenols Extraction

Pătrăuţanu¹,

Lazar²,

Popa³

et al. 2019

Cellulose Chem. Technol.

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This paper presents the influence of spruce bark (Picea abies) particle size and size distribution on the separation of polyphenols, using a green extraction process (ultrasound-assisted extraction-UAE), in order to highlight the kinetic mechanism. Experiments were performed at 50 °C, for 45 minutes, using ethanol-water (70% v/v) as solvent. The considered particle size fractions were: 0.25, 0.315, 0.4, 0.5, 0.63 and 1 mm. The crude extracts were assessed using the Folin Ciocalteu method for total polyphenols content (TPC) and high-performance thin layer chromatography (HPTLC) for identifying simple phenolic acids and tannins. The study points out that particle size significantly influences the UAE process in terms of separated polyphenols and kinetic mechanism as well. From the particles with the lowest size, the highest amounts of simple phenolic acids (sinapic acid, p-coumaric acid) and tannins (catechin) were obtained. Extraction kinetics was estimated using a second order model. Results showed a good prediction of this model for the extraction kinetics in all the experiments (R 2 > 0.911), which gave the possibility to estimate the concentration of polyphenols at saturation (C s), the rate constant (k) and the initial rate (h). All these parameters were affected by the particle size and size distribution of spruce bark.

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“…HPTLC identification and densitometric assay: HPTLC chromatographic study was done according to literature [8][9][10] using the equipment CAMAG LINOMAT IV, TLC 3 Scanner and as software WINCATS Planar Chromatography Manager.…”

Section: Qualitative Analysismentioning

confidence: 99%

Studies on Polyphenols Isolated from Branches of Prunus spinosa L. Species

Ciupercǎ¹,

Ţebrencu²,

Ionescu³

et al. 2019

Rev. Chim.

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The aim of this study was to investigate the crude extracts isolated from branches of Prunus spinosa L. (Rosaceae), in order to quantify polyphenols and tannins using instrumental methods (UV-Vis spectrometry and HPTLC densitometry). The qualitative analysis on biomass revealed the presence of valuable compounds such as polyphenols, flavonoids and tannins. HPTLC fingerprinting of crude methanol extract showed several peaks, with different Rf values, corresponding to phytocompounds such as chlorogenic acid (Rf 0.52), neochlorogenic acid (Rf 0.58), caffeic acid (Rf 0.95) and protocatechuic acid (Rf 0.96). The quantitative analysis revealed that the branches of P. spinosa contain polyphenols (2.97�0.059%g gallic acid equivalent/g dried vegetal material) and tannins (0.90�0.033%g tannic acid equivalent/g dried vegetal material). The HPTLC densitometry analysis offers information about the amount of neochlorogenic acid in the vegetal material 0.12% g/g. The content in polyphenols and tannins suggests the possibilities to use this species to separate compounds with antioxidant potential. These results open perspectives for complex valorization of Prunus spinosa species, a bioresource known only for phytotherapeutical and nutritional potential of their fruits.

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Phytochemical evaluation and HPTLC investigation of bark and extracts of Rhamnus Frangula Linn

Cited by 12 publications

References 8 publications

Contributions on Spruce Bark Polyphenols Identification Using Instrumental (UV-VIS Spectrometry), Qualitative (Thin Layer Chromatography) and Quantitative (HPTLC Densitometry) Methods

Contributions on Spruce Bark Polyphenols Identification Using Instrumental (UV-VIS Spectrometry), Qualitative (Thin Layer Chromatography) and Quantitative (HPTLC Densitometry) Methods

Influence of Particle Size and Size Distribution on Kinetic Mechanism of Spruce Bark Polyphenols Extraction

Studies on Polyphenols Isolated from Branches of Prunus spinosa L. Species

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