Morocco is known
for its high plant biodiversity, but many plants
are poorly valorized. For this reason, this study aims to valorize
the methanolic and aqueous extracts of Melitotus albus leaves by studying their antioxidant activity and toxicity. The
extracts’ antioxidant activity is assessed using the FRAP,
DPPH, CAT, and ABTS methods. The chemical composition was determined
using LC–MS analysis and evaluated using in silico studies.
The results revealed that the total polyphenol content of the aqueous
extract, 259.26 ± 7.79 (mg GAE/g), is higher than that of the
methanolic extract, 131.41 ± 12.64 (mg GAE/g). The antioxidant
activity by the methods of DPPH, ABTS, and phosphor molybdenum of
aqueous extracts (0.087 ± 0.015, 0.014 ± 0.001 and 6.157
± 1.050 mg eq vit C/g, respectively) is greater than that of
methanolic extracts (0.107 ± 0.02, 0.167 ± 0.03, and 0.453
± 0.014 mg eq vit C/g, respectively). The reducing power of iron
(FRAP) shows that the methanolic extract has a greater reducing power
than that of the aqueous extract with a low IC50 (0.011 ± 0.003
and 0.199 ± 0.016 mg/mL, respectively). The study of acute and
subacute toxicity shows that the administration of the aqueous extract
of M. albus at different doses increases
the body weight of rats without modifying their general behavior.
The M. albus extract had a 99.99% total
phenolic content, as determined by LC–MS, consisting of 12
different components. The primary constituents of the extract are
chlorogenic acid (43.68%), catechin/epicatechin (24.82%), quercetin-3-O-glucuronic acid (9.91%), naringin (7.64%), and p-hydroxybenzoic/salicylic acid (2.95%). The in-silico study
showed that these compounds can passively permeate through the blood
and have a beneficial effect on various organs of the body. Based
on these results, M. albus can be used
as a medicinal plant in phytotherapy, cosmetics, or as a dietary supplement.
The bioactive compounds of these plants will require a lot of further
effort in terms of isolation and characterization.