Phytochemical Composition of Ethanol Extract of a Cocktail Herbal Mixture (Aju Mbaise)

Abstract

Aim: This study was carried out to determine the phytochemical constituent of ethanol extract of Aju Mbaise herbal mixture.
 Study Design: In the course of the experiment, fresh samples of the plants that make up Aju Mbaise were collected and identified as Cnestis ferruginea, Xylopia aethiopica, Uvaria chamae, Palisota hirsuta, Scleria sp., Napoleona imperialis, Dialium guineense, Combretum racemosun, and Heterotis rotundifolia respectively. The fresh plants were air-dried, cut into small pieces and blended before the extraction process. Ethanol was used as the extraction solvent.
 Place and Duration of Study: The study was carried out in the Research Laboratory of the Department of Biochemistry, Faculty of Science, University of Port Harcourt, in July 2018.
 Methodology: The qualitative phytochemical analysis was determined by Standard methods described by Sofowara (1993), for testing alkaloids, flavonoids, glycosides, phenols, saponins, steroids, tannins, and terpenoids, while the quantitative phytochemical was estimated spectrophotometrically.
 Results: The phytochemical result showed the presence of alkaloids (8.69%), flavonoids (19.10%), glycosides (6.86%), hydrogen cyanide (0.92%), phenols (31.56%), saponins (0.83%), steroids (0.94%), tannins (16.80%), and terpenoids (14.31%).
 Conclusion: The study showed that ethanol extract of Aju Mbaise herbal mixture contains tremendous amount of phytochemicals.

PHYTOCHEMICAL SCREENING AND PROXIMATE ANALYSIS OF THE LEAVES OF Launaea (Lactuca) taraxacifolia.

“…In the quantitative determination of steroids, 2 g of the dried pulverized plant leaves was mixed with 95% ethanol and filtered, followed by addition of 2 ml of chromagen solution (tetramethylbenzidine solution) and the resultant solution was left undisturbed for 30 minutes. The absorbance was then read at 550 nm [17].…”

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“…Ajụ mbaise is a polyherbal formulation, composed of combination of different leaves, roots and trunk of different medicinal plants wrapped together in various proportions and taken in the form of concoctions 9,10 . This formulation is native to the Mbaise Community in Mbaise Local Government area of Imo State, Nigeria, from where it derived its name.…”

“…Reports had it that it is used for various purposes, including-enhancing labour, facilitating the expulsion of retained placenta, relieving menstrual and post-delivery pains and promoting involution of the uterus, detoxification and cleansing of the womb, reduction of stomach size following administration to women after childbirth [9][10][11][12] . More so, Ijioma et al 13 reported that the formulation had potential anti-diarrhoeal agent and anti-motility effect on the gastrointestinal tract smooth muscles.…”

Aju-Mbaise Decoction Improves Haematological and Kidney Markers in High-Fat Diet-Fed Wistar Rats

“…The different plants that constitute AMD have been identified and comprise of leaves, roots, and trunk of medicinal plants wrapped together and used to detoxify the womb after postpartum. 6 Traditional claims have it that it has antimicrobial activity against gram-negative bacteria. 7 It has been reported to be rich in phytoconstituents which exhibited antibacterial activities against different bacteria strains and as well could be toxic to the liver on chronic administration on rats.…”