Phytochemical composition, antisnake venom and antibacterial activities of ethanolic extract of Aegiphila integrifolia (Jacq) Moldenke leaves

Nascimento, Leandro S.; Nogueira-Souza, Pâmella Dourila; Rocha-Júnior, José Roberto Silva; Monteiro-Machado, Marcos; Strauch, Marcelo A.; Prado, Simone A.L.; Melo, Paulo A.; Veiga, Valdir Florêncio da

doi:10.1016/j.toxicon.2021.05.004

Cited by 7 publications

(2 citation statements)

References 69 publications

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“…The phytochemical profile of the ethanolic extract from U. sagittifolia was investigated using qualitative and quantitative approaches. Initially, the presence of a wide range of metabolites including alkaloids, saponins, terpenoids, tannins, quinones, coumarins, phenolic, flavonoids, and anthocyanins were detected [ 72 , 73 ]. The qualitative analyses were classified as strongly positive (+++), positive (++), weakly positive (+), and non-detected (-) according to Caro et al (2019) [ 20 ].…”

Section: Methodsmentioning

confidence: 99%

A First Look at the Inhibitory Potential of Urospatha sagittifolia (Araceae) Ethanolic Extract for Bothrops atrox Snakebite Envenomation

Palacios

Sacoto-Torres

Hernández-Altamirano

et al. 2022

Toxins

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Bothrops atrox snakebites are a relevant problem in the Amazon basin. In this biodiverse region, the ethnomedicinal approach plays an important role as an alternative to antivenom therapy. Urospatha sagittifolia (Araceae) is a plant used for this purpose; however, its neutralizing properties have not been scientifically accessed. To fill this gap, we investigated the ability of U. sagittifolia to modulate the catalytic activity of Bothrops atrox venom, and their toxic consequences, such as local damage and lethality. The venom profile of B. atrox was assessed by chromatography and electrophoresis. Inhibition of the three main enzymatic and medically important toxins from the venom was evaluated using synthetic substrates and quantified by chromogenic activity assays. Additionally, the neutralization of lethality, hemorrhage and edema were investigated by in vivo assays. The possible interactions between venom proteins and plant molecules were visualized by polyacrylamide gel electrophoresis. Finally, the phytochemical constituents present in the ethanolic extract were determined by qualitative and quantitative analyses. The ethanolic extract reduced the activity of the three main enzymes of venom target, achieving ranges from 19% to 81% of inhibition. Our in vivo venom neuralizations assays showed a significant inhibition of edema (38.72%) and hemorrhage (42.90%). Additionally, lethality was remarkably counteracted. The highest extract ratio evaluated had a 75% survival rate. Our data support the biomedical value of U. sagittifolia as a source of natural enzyme inhibitors able to neutralize catalytically active B. atrox venom toxins and their toxic effects.

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Section: Methodsmentioning

confidence: 99%

A First Look at the Inhibitory Potential of Urospatha sagittifolia (Araceae) Ethanolic Extract for Bothrops atrox Snakebite Envenomation

Palacios

Sacoto-Torres

Hernández-Altamirano

et al. 2022

Toxins

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“…Additionally, the synthetic compound 37 showed PLA 2 s inhibitory activity of C. atrox venom ( 164 ). The flavones pectolinarigenine ( 39 ) and hispiduline ( 40 ), isolated from A. integrifolia (Verbenaceae) from Brazil (state of Roraima), partially inhibited the PLA 2 s activities (20 and 15% respectively) and HAases in B. atrox venom with 60 and 40% inhibition, respectively ( 166 ) ( Figure 6 and Table 3 ).…”

Section: Plant Products As Antivenom Agentsmentioning

confidence: 99%

Plant-Derived Toxin Inhibitors as Potential Candidates to Complement Antivenom Treatment in Snakebite Envenomations

et al. 2022

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Snakebite envenomations (SBEs) are a neglected medical condition of global importance that mainly affect the tropical and subtropical regions. Clinical manifestations include pain, edema, hemorrhage, tissue necrosis, and neurotoxic signs, and may evolve to functional loss of the affected limb, acute renal and/or respiratory failure, and even death. The standard treatment for snake envenomations is antivenom, which is produced from the hyperimmunization of animals with snake toxins. The inhibition of the effects of SBEs using natural or synthetic compounds has been suggested as a complementary treatment particularly before admission to hospital for antivenom treatment, since these alternative molecules are also able to inhibit toxins. Biodiversity-derived molecules, namely those extracted from medicinal plants, are promising sources of toxin inhibitors that can minimize the deleterious consequences of SBEs. In this review, we systematically synthesize the literature on plant metabolites that can be used as toxin-inhibiting agents, as well as present the potential mechanisms of action of molecules derived from natural sources. These findings aim to further our understanding of the potential of natural products and provide new lead compounds as auxiliary therapies for SBEs.

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Neuroprotective and anti-inflammatory effect of pectolinarigenin, a flavonoid from Amazonian Aegiphila integrifolia (Jacq.), against lipopolysaccharide-induced inflammation in astrocytes via NFκB and MAPK pathways

Heimfarth

Nascimento

Silva

et al. 2021

Food and Chemical Toxicology

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Phytochemical composition, antisnake venom and antibacterial activities of ethanolic extract of Aegiphila integrifolia (Jacq) Moldenke leaves

Cited by 7 publications

References 69 publications

A First Look at the Inhibitory Potential of Urospatha sagittifolia (Araceae) Ethanolic Extract for Bothrops atrox Snakebite Envenomation

A First Look at the Inhibitory Potential of Urospatha sagittifolia (Araceae) Ethanolic Extract for Bothrops atrox Snakebite Envenomation

Plant-Derived Toxin Inhibitors as Potential Candidates to Complement Antivenom Treatment in Snakebite Envenomations

Neuroprotective and anti-inflammatory effect of pectolinarigenin, a flavonoid from Amazonian Aegiphila integrifolia (Jacq.), against lipopolysaccharide-induced inflammation in astrocytes via NFκB and MAPK pathways

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