Phytochemical Composition, Antioxidant Activity and Toxicity of Aqueous Extract of Picralima nitida in Drosophila melanogaster

doi:10.26538/tjnpr/v4i12.19

Cited by 4 publications

(1 citation statement)

References 19 publications

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“…Reactive oxygen species react with many components in the biological system, creating oxidative damage that requires antioxidants to mitigate 36 . Natural antioxidants empower the body's system to withstand the influx of free radicals and avoid oxidative stress 2,9 . Mushrooms are extremely nutritious foods that contain phenols, flavonoids, and other bioactive components 37 .…”

Section: Discussionmentioning

confidence: 99%

Comparative antioxidant activity and phytochemical content of five extracts of Pleurotus ostreatus (oyster mushroom)

Effiong,

Umeokwochi,

Afolabi

et al. 2024

Sci Rep

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Reactive oxygen species reacts with numerous molecules in the body system causing oxidative damage, which requires antioxidants to ameliorate. Pleurotus ostreatus, a highly nutritious edible mushroom, has been reported to be rich in bioactive compounds. This study evaluated the comparative antioxidant activity and phytochemical contents of five extracts of P. ostreatus: aqueous (AE), chloroform (CE), ethanol (EE), methanol (ME) and n-hexane (HE). The phytochemical composition and antioxidant activity of the extracts were determined using standard in-vitro antioxidant assay methods. Results showed that the extracts contained alkaloids, tannins, saponins, flavonoids, terpenoids, phenolics, cardiac glycosides, carbohydrates, anthrocyanins, and betacyanins in varied amounts. CE had the highest flavonoid content (104.83 ± 29.46 mg/100 g); AE gave the highest phenol content of 24.14 ± 0.02 mg/100 g; tannin was highest in EE (25.12 ± 0.06 mg/100 g); HE had highest amounts of alkaloids (187.60 ± 0.28 mg/100 g) and saponins (0.16 ± 0.00 mg/100 g). Antioxidant analyses revealed that CE had the best hydroxyl radical activity of 250% at 100 µg/ml and ferric cyanide reducing power of 8495 µg/ml; ME gave the maximum DPPH activity (87.67%) and hydrogen peroxide scavenging activity (65.58%) at 500 µg/ml; EE had the highest nitric oxide radical inhibition of 65.81% at 500 µg/ml and ascorbate peroxidase activity of 1.60 (iU/l). AE had the best total antioxidant capacity (5.27 µg/ml GAE at 500 µg/ml) and ferrous iron chelating activity (99.23% at 100 µg/ml) while HE gave the highest guaiacol peroxidase activity of 0.20(iU/l). The comparative phytochemical and antioxidant characteristics (IC50) of the extracts followed the order: CE > AE > EE > ME > HE. Overall, chloroform was the best extraction solvent for P. ostreatus. The high content of phenolic compounds, flavonoids, and alkaloids in P. ostreatus makes it a rich source of antioxidants and potential candidate for the development of new therapies for a variety of oxidative stress-related disorders.

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Section: Discussionmentioning

confidence: 99%

Comparative antioxidant activity and phytochemical content of five extracts of Pleurotus ostreatus (oyster mushroom)

Effiong,

Umeokwochi,

Afolabi

et al. 2024

Sci Rep

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Predicting Plasmodium falciparum kinase inhibitors from antimalarial medicinal herbs using computational modeling approach

Adelusi,

Ojo,

Bolaji

et al. 2023

In Silico Pharmacol.

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Targeting Plasmodium falciparum calcium dependent protein kinase-1: Computational modelling approach towards the discovery of antimalarial drug from medicinal herbs and clinically approved anti-malarial drugs pharmacophores

Oladipo

Adelusi

Ojo

et al. 2022

Preprint

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The Key participants in the calcium signaling process in the malaria parasite are calcium dependent protein kinases. It has been established that Plasmodium falciparum calcium dependent protein kinase 1 (PfCDPK1) is essential for the invasion of host erythrocytes as well as for the parasite's asexual development. Due to malaria's resistance to currently available anti-malarial medications, it has become necessary to create effective inhibitory agents that target invasion-dependent proteins. In this study, libraries of 856 chemicals were screened using a variety of computational methods. Physicochemical property testing, molecular docking analysis, and pharmacokinetic ADMET profiling were used to determine these drugs' capacity to inhibit PfCDPK1. 88 of the 856 compounds derived from known antimalarial botanicals and clinically approved antimalarial drug pharmacophores have demonstrated a potent inhibitory effect on PfCDPK1. Additionally, based on research done on the purported inhibitory standard of PfCDPK1 (Purfalcamine), which had a binding affinity of -8.8 KJ/mol, twelve compounds, which include 17-hydroxyazadiradione, Nimbaflavone, Dehydrocarpaine-II, Picracin, Epicatechin gallate, and Naringin, ZINC000012504271, ZINCC000100029905, ZINC000004149268, ZINC000078832372, ZINC000102739374 and ZINC000198562314 had exhibited better binding affinities (-8.8, -8.8, -9.2, -9.1, -9.3, -10.0, -8.1, -7.9, -7.9, -8.2, -8.3, -7.9 KJ/mol respectively), pharmacokinectics, and physicochemical properties. According to our findings, 88 compounds are likely pharmacological targets with domiciled PfCDPK1 inhibitory effects. Nevertheless, our findings highlight these twelve stand-out compounds, suggesting that they may be the best candidates with regard to therapeutic efficacy.

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Phytochemical Composition, Antioxidant Activity and Toxicity of Aqueous Extract of Picralima nitida in Drosophila melanogaster

Cited by 4 publications

References 19 publications

Comparative antioxidant activity and phytochemical content of five extracts of Pleurotus ostreatus (oyster mushroom)

Comparative antioxidant activity and phytochemical content of five extracts of Pleurotus ostreatus (oyster mushroom)

Predicting Plasmodium falciparum kinase inhibitors from antimalarial medicinal herbs using computational modeling approach

Targeting Plasmodium falciparum calcium dependent protein kinase-1: Computational modelling approach towards the discovery of antimalarial drug from medicinal herbs and clinically approved anti-malarial drugs pharmacophores

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