Phytochemical composition and <i>in vitro</i> screening of the antimicrobial activity of essential oils on oral pathogenic bacteria

The in vitro cytotoxic activity on human cancer cell lines of sixteen commercial EOs such as Aloysia citriodora, Boswellia sacra, Boswellia serrata, Cinnamomum zeylanicum, Cistus ladanifer, Citrus × aurantium, Citrus limon, Citrus sinensis, Cymbopogon citratus, Foeniculum vulgare, Illicium verum, Litsea cubeba, Satureja montana, Syzygium aromaticum, Thymus capitatus and Thymus vulgaris was performed using the MTT reduction assay. The screening was carried out on human cancer cells of breast adenocarcinoma (MCF7, T47D and MDA-MB-231), chronic myelogenous erythroleukemia (K562) and neuroblastoma cell lines (SH-SY5Y). C. zeylanicum and L. cubeba EOs were the most active on almost all the cell lines studied and thus could be promising as an anticancer agent. These two species showed a difference in their composition even though they belong to the Lauraceae family. Almost 57 % of the true cinnamon composition was made of (E)-cinnamaldehyde, while L. cubeba showed citral as the major compound (68.9 %). The K562 cells were the most sensitive to these oils with an IC 50 ranging from 5.2 parts-per million (ppm) (C. zeylanicum) to 11.1 ppm (L. cubeba). The latter oil also showed an important cytotoxicity on MDA-MB-231 (13.4 ppm).

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“…(Ill.ve EO: 89.8 %). The same percentage of this compound was found in other Ill.ve EOs reported in the literature …”

Section: Resultsmentioning

confidence: 97%

Chemical Composition and in Vitro Cytotoxic Screening of Sixteen Commercial Essential Oils on Five Cancer Cell Lines

Najar

Shortrede

Pistelli

et al. 2019

Chemistry & Biodiversity

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“…L. did show a positive effect on oral mucositis. Both plants contain essential oils that have been shown to have an antimicrobial activity against multidrug-resistant S. aureus (Tardugno et al 2018;Kot et al 2018).…”

Section: Discussionmentioning

confidence: 99%

How to bridge the gap? European medical plants used for treating oral mucositis: on the search for evidence

Buentzel

Bauer

Buentzel

2020

J Cancer Res Clin Oncol

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Purpose Oral mucositis is a common, painful side effect of cancer treatment-be it locoregional (e.g. irradiation) or systemic (e. g. chemotherapy). Phytotherapy is often used by patients to alleviate symptoms. However, knowledge on which medical plants are recommended by literature about Traditional European Medicine (TEM), their effect(s) on symptoms and their efficacy is severely lacking. Therefore, we developed a novel approach to assess traditional knowledge of herbals used in TEM and searched the online databases for studies reporting effects of these plants. Methods At first, online research did not yield a satisfying number of studies (MESH terms: "mucositis" OR "stomatitis" AND "herbal" OR "herbal medicine"). Trials were labelled by the country conducting the study. In parallel, we compiled a list of 78 plants recommended for treating oral mucositis by screening 14 books on TEM. Then, a "hit list" of the plants most often mentioned was composed and used further for a second online investigation using the Latin plant designations as MESH term. Studies of both online searches were pooled for analysis. Results There is a gap between traditional knowledge and trials investigating medical plants used by TEM. Overall, herbal remedies alleviate oral mucositis and especially, gingivitis well. There is good evidence for using Matricaria recutita L., Salvia officinalis L., Calendula officinalis L. and Thymus spp. L. for treating oral mucositis. Conclusion Clinical trials investigating medical plants known in TEM are rare. However, following our research strategy, we could extrapolate four plants with good evidence for alleviating symptoms of oral mucositis and gingivitis.

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“…Dental biyofilm gelişiminin kontrolü periodontal hastalık oluşumunu önleyebilir. Dental biyofilm oluşumunun engellenmesinde fenol türevleri ve doğal bileşikler de etkili olmaktadır (32) . Yamanaka ve ark.…”

Section: Discussionunclassified

Evaluation of In Vitro Antimicrobial Potentials of Dietary Phytochemicals against Periodontal Pathogens

Bali¹,

Akca²,

Erdönmez³

et al. 2018

TMCD

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ÖZAmaç: Bu çalışmada, Porphyromonas gingivalis ATCC 33277, Prevotella intermedia ATCC 25611 ve Aggregatibacter actinomycetemcomitans ATCC 43718 periodontal patojenlerine karşı gıdalarda bulunan fitokimyasallardan kuersetin, kurkumin, apigenin, pirogallol, gallik asit, luteolin ve hidroksitirozolün in vitro antimikrobiyal potansiyellerinin değerlendirilmesi amaçlanmıştır. Gereç ve Yöntem:Çalışmada agar kuyucuk difüzyon ve mikrodilüsyon yöntemleri kullanılarak fitokimyasalların antimikrobiyal potansiyelleri araştırılmıştır. Agar kuyucuk yönteminde fitokimyasalların antimikrobiyal aktiviteleri 3 mg/ml, mikrodilüsyon yönteminde ise 3, 1.5, 0.75, 0.375, 0.187, 0.093, 0.046, 0.023 ve 0.011 mg/ml konsantrasyonlarda incelenmiştir. Pozitif ve negatif kontrol olarak amoksisilin (20 µg/disk ve 0.04-0.0015 mg/ml) ve DMSO (≤%10) test edilmiştir. Bulgular: Çalışma sonuçlarına göre, fitokimyasalların çoğunun patojenlere karşı antimikrobiyal aktivite gösterdiği ve patojenler üzerinde en etkili fitokimyasalın kuersetin olduğu belirlenmiştir. Kuersetin, kurkumin, luteolin, apigenin ve gallik asit tüm patojenlere karşı en iyi antimikrobiyal potansiyeli olan fitokimyasallar olarak belirlenmiştir. Çalışmamızda, %4 (v/v) ve daha az orandaki DMSO'nun patojenler üzerinde etkisiz olduğu belirlenmiştir. Sonuç: Periodontal hastalıkların tedavisinde antibiyotik kullanımına ilave olarak, özellikle kurkumin, kuersetin, luteolin, gallik asit ve apigenin içeren gıdaların tüketimi uygun ve yararlı olabilir. Bu yüzden, ileride, güçlü antimikrobiyal ve antioksidan potansiyeli olan fitokimyasalların periodontal patojenlerin inhibisyon mekanizmalarına yönelik etkilerinin daha detaylı araştırılması ve bu fitokimyasalların etkin kullanımlarını belirlemek için ileri boyutta in vivo çalışmalar yapılması önerilmektedir. ABSTRACT Evaluation of In Vitro Antimicrobial Potentials of Dietary Phytochemicals Against Periodontal PathogensObjective: In this study, it is aimed to investigate the antimicrobial potential of the dietary phytochemicals including quercetin, curcumin, apigenin, pyrogallol, gallic acid, luteolin and hydroxytyrosol against the periodontal pathogenic bacteria including Porphyromonas gingivalis ATCC 33277, Prevotella intermedia ATCC 25611 and Aggregatibacter actinomycetemcomitans ATCC 43718. Material and Methods:The antimicrobial potential of the dietary phytochemicals was investigated by using agar well diffusion and microdilution methods. The antimicrobial activities of the phytochemicals were both studied at a concentration of 3 mg/ml and also in concentrations of 3, 1.5, 0.75, 0.375, 0.187, 0.093, 0.046, 0.023 and 0.011 mg/ml using microdilution methods, respectively. Amoxicillin (20 µg/disc, 0.04-0.0015 mg/ml) and DMSO (≤10%) were tested as positive and negative controls, respectively.Results: According to the results of this study, most of the phytochemicals had antimicrobial activity aganist all of the bacteria. The most effective phytochemical against pathogens was quercetin. The phytochemicals with the strongest antimicrobial pot...

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Phytochemical composition and in vitro screening of the antimicrobial activity of essential oils on oral pathogenic bacteria

Cited by 66 publications

References 36 publications

Chemical Composition and in Vitro Cytotoxic Screening of Sixteen Commercial Essential Oils on Five Cancer Cell Lines

Chemical Composition and in Vitro Cytotoxic Screening of Sixteen Commercial Essential Oils on Five Cancer Cell Lines

How to bridge the gap? European medical plants used for treating oral mucositis: on the search for evidence

Evaluation of In Vitro Antimicrobial Potentials of Dietary Phytochemicals against Periodontal Pathogens

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