Phytochemical Composition and Evaluation of the Antioxidant Activity of the Ethanolic Extract of Calendula suffruticosa subsp. suffruticosa Vahl

Abstract

Aim: Calendula suffruticosa ssp. suffruticosa Vahl, Medicinal and Aromatic plant, spontaneous and widespread in the Mediterranean region and in the Northeast of Algeria, is widely used by the local population for its medicinal properties. Methods: The chemical composition of the ethanolic extract of Calendula suffruticosa was established for the first time using GC / MS. The antioxidant activity of the ethanolic extract was evaluated in vitro by the DPPH (2,2-diphenyl-1-picrylhydrazyl) test, the FRAP assay and β-carotene bleaching assay. Results: sixty-four compounds are identified by GC/MS analysis, six are predominant: α-linoleic acid (24.20%), linoleic acid (23.58%), Stigmasterol (8.53%), 2-Metyl-Z-4-tetradecene (6.62%) And α-Bisabolol (1.70%). The free radical scavenging by DPPH method indicated that the ethanolic extract of C. suffruticosa showed a very high antioxydant activity with an IC 50 = 0.017 ± 1.00 mg / ml. This extract also showed an ability to reduce very high iron with a maximum optical density of 1.157 at a concentration of 0.5 mg / ml and an antioxidant activity compared to the β-carotene / linoleic acid test of order of 75 ± 1.02%. Conclusion: These results show for the first time the richness of phytochemicals and the strong antioxidant activity of the species Calendula suffruticosa endemic to north-east of Algeria.

Antibacterial activity and essential oil composition of Calendula arvensis L.

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“…R.T: Retention time; 2 RRI: Relative retention time; 3 RRI Lit. : Relative retention time in the literature;4 The analysis results;5,6 The average % area of analysis with ± standard deviation (SD) (n=3);7 Identification method.…”

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“…Our findings suggest that hydroxyl groups could intervene as electron donors, transforming free radicals into much more stable substances by scavenging radicals. According to the literature [ 34 ], the methanolic extract of this plant had higher DPPH scavenging activity (IC 50 = 0.041 mg·mL −1 ) than the hydro-methanol extract of C. officinalis leaves (0.57 mg·mL −1 ) and lower than that of flowers (0.35 mg·mL −1 ).…”

Therapeutic Potential of Selected Medicinal Plants Against Carrageenan Induced Inflammation in Rats

“…β-Carotene bleaching assay. β-Carotene bleaching assay was performed by following the procedure of Ismahene et al 21 The absorbance of reaction mixture was noted at 0 min and after 2 h subsequently against blank containing emulsion without β-carotene at 470 nm.…”