Phytochemical composition and biological activities of native and in vitro-propagated Micromeria croatica (Pers.) Schott (Lamiaceae)

Phenolic Constituents of Lamium album L. subsp. album Flowers: Anatomical, Histochemical, and Phytochemical Study

“…The content of phenolic compounds in corolla epidermis, its structures, and the stamens was determined using fresh free-hand sections and the following histochemical assays: ferric trichloride for total phenolic compounds [64]; potassium dichromate for tannins [65]; and Toluidine blue O (pH 4) for phenolic compounds, tannins [66][67][68][69], and pectins, the staining of which is limited at their lower content in the cell walls [68,70] (pp. [25][26][27][28][29][30][31][32][33][34][35][36][37][38][39][40].…”

Glandular Trichomes on the Leaves of Nicotiana tabacum: Morphology, Developmental Ultrastructure, and Secondary Metabolites

“…The use of Nadi reagent to demonstrate different terpenoids in plant secretions was first reported for Pinus pinaster resin duct cells [121]. Since then, the presence of terpenoids (resiniferous acids and essential oils) using Nadi reagent was revealed in the trichome glandular cells or in their secretion in a number of plant species belonging to Compositae [122], Lamiaceae [12,25,123,124], Asteraceae [125], Solanaceae [58,119,126], Orobanchaceae [127], Cucurbitaceae [36], Bignoniaceae [13], Boraginaceae [128], Plantaginaceae [129], and others. Diterpenes found in the leaf surface gum of N. tabacum are produced and secreted by the tall glandular trichomes but not by the short ones that do not exhibit resinous material on their surfaces [3,45].…”

“…Reports on the histochemical detection of different secondary metabolites carried out in plantlets grown in vitro are scarce [34,124,130]. Plants grown in vitro are continuously exposed to a defined microenvironment, providing nearly optimal conditions for plant proliferation, in contrast to marked variations of the growth conditions of field-grown plants.…”

“…Secondary metabolite production can be drastically altered in response to different plant growth regulators in the culture medium [139,[141][142][143][144]. Exogenous application of cytokinins has been shown to affect the essential oil production, often generating higher essential oil yield [124,145,146]. The availability of carbon, nitrogen, and sulfur resources, having a large impact on the production of specific classes of primary metabolites, consequently affects the levels and composition of secondary metabolites derived from these primary metabolites [97,147].…”

Total flavonoid, phenolic and antioxidant activities of Pelargonium quercetorum Agnew: Comparison of in vivo and in vitro grown plant

“…Micropropagation techniques or in vitro cultures; unlike other classical production methods, are the process of culturing explants taken from various parts of the plant in sterile environments and aseptic conditions after sterilization and transforming these explants into plantlets in this environment (Başak and Candan, 2008). In previous studies, it has been reported that the production of secondary metabolites is promoted by plant tissue culture techniques and cause to differences in antioxidant systems (Tošić et al, 2019;Yaman et al, 2020).…”