Phytochemical composition and antioxidant capacity of the aqueous extracts of Malva sylvestris L. and Malva pseudolavatera Webb & Berthel.

Abstract

Context: Malva spp. have been widely used in the world as traditional remedies. In Ecuadorian markers Malva sylvestris and Malva pseudolavatera are the species most commercialized. However, the scientific information about M. pseudolavatera is little. Aims: To determine the chemical composition and antioxidant capacity of the aqueous extracts of the leaves of both species. Methods: Aqueous extracts were partitioned with butanol and the fractions obtained were analyzed by GC/MS. Total extracts were evaluated as antioxidants using FRAP, DPPH and ABTS assays. Results: Differences in the chemical composition were found between the extracts. Both extracts showed the presence of polysaccharides, phenolic acids and fatty acids, but in M. sylvestris were identified polyols, which there was not observed in M. pseudolavatera. The aqueous extracts showed free radical scavenging and ferric reducing power capacities in vitro assays, being M. sylvestris the most promissory antioxidant, possibly due to the presence of polyols in the extract. In the present research were reported 39 phytochemical compounds by first time for the specie M. pseudolavatera. Conclusions: Aqueous extracts of the leaves of M. sylvestris and M. pseudolavatera showed antioxidant capacity associated with the presence of phenolic acids, polysaccharides and flavonoids in the extracts.

Extraction of Bioactive Compounds from Prestonia mollis Leaves and Their Impregnation into Polylactic Acid Using High-Pressure Technologies: Potential for Biomedical Application

“…Malva sylvestris and Malva pseudolavatera species are the main ones sold in Ecuadorian markets. Antioxidant activity measurements by DPPH and ABTS have shown that the aqueous extract of M. sylvestris (IC 50 DPPH = 78.14 µg/mL; IC 50 ABTS = 166.79 µg/mL) was the most antioxidant extract, probably due to the presence of polyols [48]. These results corroborate how important it is to select the appropriate extraction technique as well as the solvent, as they have a direct influence on the bioactive compounds to be obtained.…”

In vitro antioxidant properties and antimicrobial activity of the ethanolic extract of Senecio nutans Sch. Beep. (Asteraceae)

“…Trolox was used as a reference compound in a concentration range of 0.0312-0.5 mM. Results were expressed as mmol Trolox equivalents/g (TEAC: Trolox equivalent antioxidant capacity) (Ramos-Escudero et al, 2012;Sarmiento et al, 2022).…”

“…The antioxidant activity was expressed as the value of the 50% inhibitory concentration (IC50). The DPPH radical solution was prepared at a concentration of 100 mM in 80%( v /v) methanol (Ramos-Escudero et al, 2012;Sarmiento et al, 2022).…”

“…Results are expressed as mM Trolox/g dry weight of plant extract. The FRAP solution was prepared by mixing 25 mL of acetate buffer (300 mM, pH 3.6), 2.5 mL of 10 mM TPTZ (2,4,6-tripyridyl-s-triazine) solution diluted with 40 mM HCl and trichloride ferric solution (FeCl3.6H2O) 20 mM (Ramos-Escudero et al, 2012;Sarmiento et al, 2022). All the antioxidant tests were performed in triplicate.…”