Phytochemical composition and antioxidant activities of leaf extracts of <i>Viola odorata</i> from Kishtwar, Jammu and Kashmir

Aslam, Lubna; Kaur, Ramanjeet; Kapoor, Nisha; Mahajan, Ritu

doi:10.1080/10496475.2019.1677839

Cited by 10 publications

(3 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…11 Prior studies have demonstrated TPC of extracts from Viola species broadly ranged between 0.77 and 48.18 mg GAE/g. 23,[28][29][30][31] Generally, TPC of the V. dalatensis acetonic extract fell within the reported range.…”

Section: Phenolicssupporting

confidence: 68%

Pigments, Phenolics, Antioxidant Activity, Anti--Amylase, and In Vitro Anti-Inflammatory Properties of Crude Acetonic Extract from Viola dalatensis

2023

TJNPR

View full text Add to dashboard Cite

V. yedoensis n-butanol extract inhibited the production of Hepatitis B surface antigen (HBsAg) and Hepatitis B e antigen (HBeAg) in HepG2.2.15 cells, and hindered the replication of Hepatitis B virus DNA. 8 Additionally, the extract demonstrated protective effects against immunological liver injury in an in vivo study. The authors postulated that this hepatoprotection might be associated with coumarin and flavonoid compounds detected in the extract. Viola dalatensis Gagnep., is a short-stemmed herbaceous perennial with light violet flowers and a 1.5 mm spur. It was first identified and documented by Gagnepain in 1938 and is exclusively found in the southern regions of Vietnam. 9 In the present study, we explored chlorophyll, carotenoids, and phenolics of V. dalatensis aerial parts. Besides, the antioxidant activity, anti--amylase and in vitro antiinflammatory properties of the acetonic extract of the plant were evaluated. The findings of this study will give a better understanding of the bioactive compounds and potential health-endorsing activities of V. dalatensis. Materials and Methods ChemicalsAcetone was obtained from VWR Chemicals (France). Ferulic acid and gallic acid were obtained from Sigma-Aldrich (Missouri, USA). Catechin, epicatechin, quercetin, and rutin were procured from Chengdu Biopurify Phytochemicals (Sichuan, China). Chlorogenic acid, kaempferol, and diclofenac were procured from the National Institute of Drug and Quality Control (Hanoi, Vietnam). Acarbose (50 mg tablet, Khapharco Pharmaceuticals Company) was purchased from a drug store in Ho Chi Minh City, Vietnam. Plant Collection and IdentificationViola dalatensis fresh aerial parts were collected in Lam Dong, Vietnam (12°05'47.6"N; 108°22'35.4"E) on April 15, 2022. The plant species was authenticated by Van-Son Dang at the Institute of Tropical Biology, VAST, Vietnam. The voucher specimen (HieuTT-15042022

show abstract

Section: Phenolicssupporting

confidence: 68%

Pigments, Phenolics, Antioxidant Activity, Anti--Amylase, and In Vitro Anti-Inflammatory Properties of Crude Acetonic Extract from Viola dalatensis

2023

TJNPR

View full text Add to dashboard Cite

show abstract

“…Previously, V. odorata leaves were extracted with methanol and analyzed for total metabolites by Aslam et al (2020). It was reported that the total avonoid contents in the methanol extract were 7.5 mg QE g DW − 1 , and the total phenolic was 1.06 mg GAE g DW − 1 .…”

Section: Discussionmentioning

confidence: 99%

“…It was reported that the total avonoid contents in the methanol extract were 7.5 mg QE g DW − 1 , and the total phenolic was 1.06 mg GAE g DW − 1 . The ethanol extract of V. odorata leaves was analyzed, and alkaloids, terpenoids, and saponins were con rmed (Aslam et al 2020). Akhbari et al ( 2012) prepared a methanolic extract from V. odorata aerial parts.…”

Section: Discussionmentioning

confidence: 99%

A rationally optimised batch bioreactor cultivation of Viola odorata plant cells for sustainable production of its key bioactive principles

Srivast

2024

Preprint

View full text Add to dashboard Cite

Viola odorata plant extracts are extensively used in indigenous medicine to treat infectious diseases. V. odorata's limited availability in nature, due to geographical conditions and adulteration, makes continuous supply of high-quality plant raw material challenging. This results in low-quality plant extracts that can have inconsistent and sometimes adverse effects when used in medicine. As an effective solution, in vitro plant biomass cultivation conserves plant diversity, ensuring high-quality raw materials with uniform quality and quantity. Toward this, the current study focuses on establishing bioprocess strategy to achieve maximum V. odorata biomass productivity up to bioreactor level. In this context, in vitro culture conditions were rationally optimised, focusing upon both statistical optimization and one-factor-at-a-time (OFAT) approach, for improved biomass productivity. In particular, basal medium, carbon and nitrogen source, inoculum density, macronutrient concentration, shaking speed, temperature, pH, light intensity, and plant growth regulators were systematically optimized in order to achieve the maximum biomass. This led to increased biomass production from 10.2 ± 0.8 g DW L-1 to 21.68 ± 0.82 g DW L-1 within 12 days of the batch cultivation period. Optimized culture conditions were implemented in three different types of bioreactors, namely, stirred tank, airlift, and bubble column, to cultivate V. odorata biomass. Of these three methods, cultivation in a stirred tank reactor produced ~19.7 g DW L-1 of maximum biomass within a 12-day batch cultivation period. Further, the metabolite profile of the bioreactor cultivated biomass (alcoholic extract) was compared with that of the natural plant biomass using gas chromatography-mass spectrometry based metabolomics, yielding around 18 key principles (metabolites) that exhibit resemblance with that in the natural source. The antibacterial activity of the plant extract was tested against three bacteria responsible for respiratory tract infections, and their antioxidant activity was compared with that of Vitamin E, wherein the results demonstrated comparable activity. The novelty of this study is in the fact that the V. odorata plant cells were cultivated in bioreactors for the first time for potential medicinal applications. It provides an alternative method for sustainable production of plant biomass, thereby conserving the natural plant sources.

show abstract

Synthetic Biology of Plants-Derived Medicinal Natural Products

Nipunta,

Kaur,

Sagar

et al. 2024

Biosynthesis of Natural Products in Plants

View full text Add to dashboard Cite

Phytochemical composition and antioxidant activities of leaf extracts of Viola odorata from Kishtwar, Jammu and Kashmir

Cited by 10 publications

References 18 publications

Pigments, Phenolics, Antioxidant Activity, Anti--Amylase, and In Vitro Anti-Inflammatory Properties of Crude Acetonic Extract from Viola dalatensis

Pigments, Phenolics, Antioxidant Activity, Anti--Amylase, and In Vitro Anti-Inflammatory Properties of Crude Acetonic Extract from Viola dalatensis

A rationally optimised batch bioreactor cultivation of Viola odorata plant cells for sustainable production of its key bioactive principles

Synthetic Biology of Plants-Derived Medicinal Natural Products

Contact Info

Product

Resources

About