Phytochemical Composition and Antimicrobial Activity of Essential Oil from the Leaves of Artemisia vulgaris L.

Abstract

Artemisia vulgaris is an enormously useful aromatic plant known for its insecticidal, antifungal, parasiticidal, and medicinal values. The main aim of this study is to investigate phytochemical contents and the potential antimicrobial activities of Artemisia vulgaris essential oil (AVEO) from the fresh leaves of A. vulgaris grown in Manipur. The AVEO isolated by hydro-distillation from A. vulgaris were analyzed by gas chromatography/mass spectrometry and solid-phase microextraction-GC/MS to describe their volatile chemical profile. There were 47 components identified in the AVEO by GC/MS, amounting to 97.66% of the total composition, while 97.35% were identified by SPME-GC/MS. The prominent compounds present in AVEO analyzed by direct injection and SPME methods are found to be eucalyptol (29.91% and 43.70%), sabinene (8.44% and 8.86%), endo-Borneol (8.24% and 4.76%), 2,7-Dimethyl-2,6-octadien-4-ol (6.76% and 4.24%), and 10-epi-γ-Eudesmol (6.50% and 3.09%). The consolidated component in the leaf volatiles comes to the terms of monoterpenes. The AVEO exhibits antimicrobial activities against fungal pathogens such as Sclerotium oryzae (ITCC 4107) and Fusarium oxysporum (MTCC 9913) and bacterial cultures such as Bacillus cereus (ATCC 13061) and Staphylococcus aureus (ATCC 25923). The percent inhibition of AVEO against the S. oryzae and F. oxysporum was found up to 50.3% and 33.13%, respectively. The MIC and MBC of the essential oil tested for B. cereus and S. aureus were found to be (0.3%, 0.63%) and (0.63%, 2.5%), respectively. Finally, the results revealed that the AVEO characterized by the hydro-distillation and SPME extraction yielded the same chemical profile and showed potent antimicrobial activities. Further research into A. vulgaris’s antibacterial properties can be performed in order to use it as a source for natural antimicrobial medications.

Artemisia arborescens (Vaill.) L.: Micromorphology, Essential Oil Composition, and Its Potential as an Alternative Biocontrol Product

“…To find and ascertain the antibacterial activity of essential oils, recently, numerous studies have been carried out [ 50 ]. Although the exact mechanism of action is still unknown, several investigations have indicated that essential oil components may enter cells and disrupt cellular metabolism [ 51 ]. Phenols, such as eugenol and carvacrol, damage cellular membranes and interact with enzyme-active sites.…”

Preliminary Study on Insecticidal Potential and Chemical Composition of Five Rutaceae Essential Oils against Thrips flavus (Thysanoptera: Thripidae)

“…The main constituents of Zanthoxylum bungeanum essential oil, extracted using a low-eutectic solvent and steam distillation, were terpineol-4-ol (13.13%) and (–)-β-pinene (11.17%) [ 51 ]. Many studies have shown that differences in the prominent essential oil compounds could be explained by variables such as harvest time, plant ontogeny, geographical location, plant parts, as well as extraction techniques [ 52 , 53 , 54 , 55 , 56 ].…”

Nutritional properties of giant water bug, Lethocerus indicus a traditional edible insect species of North-East India

“…The GC-MS employed have Trace1300 as Gas chromatography and TSQ DUO as Mass Spectrophotometry. The GC have the silica capillary column (30 m X 0.25 mm; 0.25 µm film thickness), TG-5 MS interfaced with the MS.The carrier gas used in the instrument was helium with a flow rate of 1 ml/min for 60 minutes [ 18 , 19 ]. The ionization energy was set at 70 eV, and the mass transfer line temperature at 250°C and the ion source temperature at 280°C.…”

“…The ionization energy was set at 70 eV, and the mass transfer line temperature at 250°C and the ion source temperature at 280°C. The column temperature was programmed from 40°C for 1 min to 280°C at the rate of 5°C/min by a heating ramp for 20 min and held at 250°C.The inlet injector temperature was set at 240°C with a split mode of 1:20 maintained [ 18 , 19 ]. The mass spectra were filtered from the mass range from 35 to 450 Mw.The identification of the volatile compound was determined by comparing the spectra obtained from the sample with the reference to the mass spectrum given by the National Institute of Standards and Technology (NIST) GC/MS Libraries 2017 [ 19 ].…”