Phytochemical Composition and Antimicrobial Activity of Corydalis solida and Pseudofumaria lutea

Abstract

Corydalis and Pseudofumaria are two closely related genera from the Papaveraceae subfamily Fumarioideae with Corydalis solida (C. solida) and Pseudofumaria lutea (P. lutea) as two representative species. Phytochemical analysis revealed significant differences in the quality and quantity of isoquinoline alkaloids, phenolic compounds and non-phenolic carboxylic acids between aerial and underground parts of both species. Using the Liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) technique, 21 compounds were identified: five protoberberine derivatives, three protopine derivatives, four phenanthridine derivatives, as well as three carboxylic acids, two hydroxycinnamic acids, one chlorogenic acid, one phenolic aldehyde, and two flavonoids. Moroever, significant differences in the content of individual compounds were observed between the two studied species. The phytochemical profile of C. solida showed a higher variety of compounds that were present in lower amounts, whereas P. lutea extracts contained fewer compounds but in larger quantities. Protopine was one of the most abundant constituents in C. solida (440–1125 µg/g d.w.) and in P. lutea (1036–1934 µg/g d.w.). Moreover, considerable amounts of coptisine (1526 µg/g) and quercetin (3247 µg/g) were detected in the aerial parts of P. lutea. Extracts from aerial and underground parts of both species were also examined for the antimicrobial potential against S. aureus, P. aeruginosa and C. albicans. P. lutea herb extract was the most effective (MIC at 0.39 mg/L) against all three pathogens.

Antibiofilm and Antimicrobial-Enhancing Activity of Chelidonium majus and Corydalis cheilanthifolia Extracts against Multidrug-Resistant Helicobacter pylori

“…Flowering plants were harvested in April 2018: C.majus on 27 April; C.cheilanthifolia and F.vaillantii on 21 April. Flowering P. lutea was harvested on 20 April 2018, as previously reported by Zielińska et al (2020) [ 18 ] and flowering F. officinalis was harvested on 13 May 2020 and flowering G. flavum was harvested on 3 June 2020. Voucher specimens of the species were deposited in the Herbaria of Botanical Gardens, described above, under the codes: L02/C.m.1-15/18; L01/C.ch.1-11/18; L01/F.v.1-10/18; P.l.—4223A; L01/F.o.1-10/20; G.f.—OB-6777-R, respectively.…”

“…Based on a review of the literature and our previous results showing the antimicrobial activity of plant extracts from the Papaveraceae family [ 18 , 19 , 20 , 21 ], in the present research article we decided to verify this activity against H. pylori because such analyses were not performed in this respect so far. For this purpose, we chose the multidrug-resistant H. pylori 8064 strain, characterized by resistance to all three classically used antibiotics (CLR, LEV, and MTZ) but sensitive to amoxicillin (AMX) [ 22 ].…”

“…The phytochemical analyses previously conducted for the studied plants showed that they contain different proportions of various phenolic compounds, but most of all they contain isoquinoline alkaloids, such as protoberberine, protopine, and benzophenanthridine derivatives [ 18 , 19 , 36 , 37 , 38 ]. This is a large class of alkaloids that occurs in several plant families, such as Papaveraceae, Fumariaceae, Berberidaceae, and Rutaceae [ 39 ].…”

“…Due to its lipophilicity, SER shows the ability to cross the membrane barrier independent of porins [44], and hence perhaps, the presence of isoquinoline alkaloids from both tested extracts may be less significant in enhancing its antimicrobial activity. The phytochemical analyses previously conducted for the studied plants showed that they contain different proportions of various phenolic compounds, but most of all they contain isoquinoline alkaloids, such as protoberberine, protopine, and benzophenanthridine derivatives [18,19,[36][37][38]. This is a large class of alkaloids that occurs in several plant families, such as Papaveraceae, Fumariaceae, Berberidaceae, and Rutaceae [39].…”

Antimicrobial and Anticancer Activity of Corydalis solida

“…C. solida has been used in folk medicine as a sedative, pain reliever, and blood pressure reducer. Because it has starchrich tubers, it was cooked and eaten (1). The Papaveraceae family of plants is of great interest in the field of medical use due to their content with significant bioactivity.…”