Phytochemical Characterization of By-Products of Habanero Pepper Grown in Two Different Types of Soils from Yucatán, Mexico

Abstract

By-products of edible plants may contain potentially useful phytochemicals. Herein, we valorized the by-products of Capsicum chinense by phytochemical characterization of its leaves, peduncles and stems. Plants of habanero pepper were grown in a greenhouse, in polyethylene bags with two soils that were named according to the Maya classification as: K’ankab lu’um (red soil) and Box lu’um (black soil). Habanero pepper by-products were dried using an oven, the extracts were obtained by Ultrasound Assisted Extraction, and phytochemical quantification in all the extracts was conducted by Ultra Performance Liquid Chromatography coupled to Diode Array Detector (UPLC-DAD). Differences in the phytochemical content were observed according to the by-product and soil used. Catechin and rutin showed the highest concentrations in the peduncles of plants grown in both soils. The leaves of plants grown in black soil were rich in myricetin, β-carotene, and vitamin E, and the stems showed the highest protocatechuic acid content. While the leaves of plants grown in red soil were rich in myricetin and vitamin C, the stems showed the highest chlorogenic acid content. This novel information regarding the phytochemical composition of the by-products of C. chinense may be relevant in supporting their potential application in food and pharmaceutical industries.

Ultrasonic-assisted extraction of polyphenolic compounds from Paederia scandens (Lour.) Merr. Using deep eutectic solvent: optimization, identification, and comparison with traditional methods

“…Moreover, there was no significant difference between ABTS + value obtained by DES-UAE and W-UAE ( p > 0.05), whereas DES-UAE displayed significantly higher ABTS + value than other methods ( p < 0.05). These results indicated that DES-UAE was a high efficiency method compared with UAE coupled with traditional solvents (ultrapure water, 80% ethanol, and 70% methanol), which were usually used in the previous studies and showed a high efficiency for extracting polyphenols [27] , [32] , [33] , [34] , [35] , [30] , [36] . DES-UAE reportedly improved TFC and antioxidant capacity of Moringa oleifera L. leave extracts, compared with UAE coupled with traditional solvents [29] , [22] .…”

“…The supernatant was obtained by centrifugation at 13,000 rpm for 10 min. The traditional solvents used were chosen based on previous studies [27] , [32] , [33] , [34] , [35] , which were efficient in polyphenolic extraction.…”

In Vivo Anti-Inflammatory Effect, Antioxidant Activity, and Polyphenolic Content of Extracts from Capsicum chinense By-Products

“…The column was an Acquity UPLC HSS C18 (100 A • , 1.8 µm, 2.1 mm × 50 mm) (Waters, Milford, MA, USA). Validation of the chromatographic method was carried in accordance with the Eurachem Guide: The Fitness for Purpose of Analytical Methods-A Laboratory Guide to Method Validation and Related Topics [49] reported in a previous work conducted by Chel-Guerrero et al [50]. Supplementary Materials Table S1 contains the validation values, as well as the chromatographic parameters of the method.…”

Effect of the Soil and Ripening Stage in Capsicum chinense var. Jaguar on the Content of Carotenoids and Vitamins

“…Analysis of metabolites (individual carotenoids and vitamins) was performed in an UPLC Acquity H Class (Waters, Milford, MA, USA) with a diode array detector and an Acquity UPLC HSS C18 column (100 Å, 1.8 µm, 2.1 × 50 mm) (Waters, Milford, MA, USA). Validation of the chromatographic methods was carried in accordance with the Eurachem Guide: The Fitness for Purpose of Analytical Methods-A Laboratory Guide to Method Validation and Related Topics [27] reported in a previous work conducted by Chel-Guerrero et al [28].…”