Phytochemical Characterization of Bioactive Compounds on Ethanol Extracts Fermentation Products of Isolate BJDTA21 Endophytic Bacterial Andalas

Abstract

The West Sumatra, Indonesia, mascot plant is called Andalas (Morus macroura Miq.). This plant belongs to the Moraceae family, which has a long history of being used medicinally. The goal of this study was to evaluate the Andalas ethanol extract's potential to prevent the growth of pathogenic microorganisms. The Andalas stems and roots were subjected to the ethanol extraction process. A completely randomized design (CRD) was utilized to determine the ideal concentration of ethanol extracts of Andalas roots and stems for suppressing test microorganisms. In this investigation, there were four different extract concentrations used as treatments: 3.12 percent, 6.25 percent, 12.50 percent, and 25 percent. The paper disc diffusion method was used to conduct antimicrobial activity testing on the test microorganisms (Staphylococcus aureus, Escherichia coli, and Candida albicans). The outcomes revealed that the. The extract's potential mechanisms for exerting antibacterial activity against both of the studied microorganisms include flavonoids, alkaloids, saponins, terpenoids, and tannins.