Phytochemical characterization, in vivo and in vitro biological activities of Atriplex lindleyi Moq. subsp. inflata

Meguellati, Hassina; Ouafi, Saida; Saad, Somia; Harchaoui, Lylia; Djemouai, Nadjette

doi:10.1016/j.jep.2023.117333

Cited by 3 publications

(1 citation statement)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In order to characterize the phenolic composition of the various extracts of C. violacea (Desf.) Beck, a high-performance liquid chromatography system (Agilent 1100) was used, as described in Meguellati et al (19). For this, a Hypersil BDS C18 analytical column (4.6 X 250 mm and 5 µm particle size, a UV Diode Array Detector (DAD) ranging from 200 to 400 nm and equipped with a quaternary rapid separation pump were used.…”

Section: High-performance Liquid Chromatography (Hplc) Analysismentioning

confidence: 99%

The Parasitic Plant Cistanche violacea (Desf.) Beck from Ghardaïa (Algeria): A Source of Biologically Active Compounds

Djemouaı,

Saad,

Baali

et al. 2024

Journal of the Turkish Chemical Society Section A: Chemistry

Self Cite

View full text Add to dashboard Cite

The present study aims to determine the contents of total polyphenol (TPC) and total flavonoid (TFC) and evaluate the antioxidant and antimicrobial activities of Cistanche violacea (Desf.) Beck. The extraction of bioactive compounds from the whole plant of C. violacea (Desf.) Beck was carried out by three different methods, including maceration, Soxhlet, and ultrasound, with ethanol and water as solvents. The results showed that the ultrasound ethanolic extract recorded the greatest amounts of TPC with 188.95 ± 2.2μg gallic acid equivalent/mg dry extract, while for TFC, it was the maceration ethanolic extract with 40.26 ± 6.02µg quercetin equivalent/mg dry extract. The High-Performance Liquid Chromatography (HPLC) results showed the presence of phenolic compounds, with 32 identified compounds in the ethanolic and aqueous extracts. The different extracts showed antioxidant capacities for DPPH, ABTS, phenanthroline, and reducing power assays. The highest antioxidant ability was observed with the ethanolic extract obtained by the maceration method (IC50 33.35 ± 1.4 and IC50 < 12.5 μg/mL for DPPH and ABTS tests). The antimicrobial investigation on seven microbial strains revealed that the ethanolic extract ultrasound showed moderate antibacterial activity (18.66 ± 1.1mm) against only Staphylococcus aureus ATCC25923, while the other extracts showed weak or no antimicrobial activity. This work suggests that C. violacea (Desf.) Beck has antioxidant properties of natural origin, which justify its traditional use in treating many conditions related to oxidative stress.

show abstract

Section: High-performance Liquid Chromatography (Hplc) Analysismentioning

confidence: 99%